A5072798069- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Nerve injury and regeneration
- Retinal Development and Disorders
- Graphene and Nanomaterials Applications
- Electrospun Nanofibers in Biomedical Applications
- Polymer Surface Interaction Studies
- Tissue Engineering and Regenerative Medicine
- Hydrogels: synthesis, properties, applications
- Wound Healing and Treatments
- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- Pulmonary Hypertension Research and Treatments
- Tactile and Sensory Interactions
- Biomedical Ethics and Regulation
- Protein Degradation and Inhibitors
- Cardiac Valve Diseases and Treatments
- Inflammatory Bowel Disease
- Photoreceptor and optogenetics research
- Skin Protection and Aging
- Autoimmune and Inflammatory Disorders
- Liver physiology and pathology
- Angiogenesis and VEGF in Cancer
- Silk-based biomaterials and applications
- Diverticular Disease and Complications
Durham University
2019-2024
University of California, Los Angeles
2014-2023
University of Washington
2020
University of Colorado System
2019
University of Colorado Anschutz Medical Campus
2019
University of Colorado Denver
2019
University of Colorado Boulder
2012
Abstract Recreating the structure of human tissues in laboratory is valuable for fundamental research, testing interventions, and reducing use animals. Critical to such technology ability produce tissue models that accurately reproduce microanatomy native tissue. Current artificial cell‐based skin systems lack thorough characterisation, are not representative skin, can show variation. In this study, we have developed a novel full thickness model comprised epidermal dermal compartments. Using...
The Caco-2 monolayer is the most widely used in vitro model of human intestinal mucosa to study absorption. However, models lack communication from other cells present native intestine, such as signals fibroblasts lamina propria. In this study, we have investigated effects upon epithelium through two mechanisms: indirect signalling and direct contact with fibroblasts. Culture paracrine fibroblasts, use conditioned media, did not induce a significant change epithelial cell morphology or...
Abstract Macroporous scaffolds are being increasingly used in regenerative medicine and tissue repair. While the recently developed microporous annealed particle (MAP) have overcome issues with injectability situ hydrogel formation, limitations respect to tunability be able manipulate strength rigidity for broad applications still exist. To address these key issues, here microparticles (HMPs) of hyaluronic acid (HA) synthesized using thiol‐norbornene click reaction then HMPs subsequently...
Spatially patterned hydrogels are becoming increasingly popular in the field of regenerative medicine and tissue repair because their ability to guide cell infiltration migration. However, postfabrication technologies usually required spatially pattern a hydrogel, making these difficult translate into clinic. Here, an injectable hydrogel is reported using hyaluronic acid (HA)-based particle hydrogels. These sequentially loaded syringe form and, once injected, they maintain pattern. The...
A major challenge in regenerative medicine is replacing cells lost through injury or disease. While significant progress has been made, much remains unknown about the accuracy of native programs cell replacement. Here, we capitalized on capacity and stereotypic retinal organization zebrafish to determine specificity with which Müller glial replace neuronal types. By utilizing a targeted genetic ablation technique, restricted death all distinct cone photoreceptor types (red, blue,...
Abstract Human skin equivalents (HSEs) are an increasingly popular research tool due to limitations associated with animal testing for dermatological research. They recapitulate many aspects of structure and function, however, only contain two basic cell types model dermal epidermal compartments, which limits their application. We describe advances in the field tissue modeling produce a construct containing sensory‐like neurons that is responsive known noxious stimuli. Through incorporation...
Angiogenesis after stroke is correlated with enhanced tissue repair and functional outcomes. The existing body of research in biomaterials for focuses on hydrogels the delivery stem cells, growth factors, or small molecules drugs. Despite ability to enhance all these methods, no material has significantly regrown vasculature within translatable timeline days weeks stroke. Here, two novel biomaterial formulations granular are developed regeneration stroke: highly porous microgels (i.e., Cryo...
Abstract The ability to design artificial extracellular matrices as cell‐instructive scaffolds has opened the door technologies capable of studying fate cells in vitro and guiding tissue repair vivo. One main component is incorporation biochemical cues guide cell phenotype multicellular organization. matrix (ECM) composed a heterogeneous mixture proteins that present variety spatially discrete signals residing populations. In contrast, most engineered ECMs do not mimic this heterogeneity....
At present, preclinical models of inflammatory bowel disease (IBD) are insufficient, limiting translation between research and new therapeutics. This is especially true for fistulising Crohn's (CD), as the severe lack relevant hinders progression. To address this, we present in vitro human IBD mucosal that recapitulate multiple pathological hallmarks simultaneously one model system - immune cell infiltration, stromal remodelling epithelial disruption. Stimulation induces aberrations common...
Angiogenesis after stroke is correlated with enhanced tissue repair and functional outcomes. The existing body of research in biomaterials for focuses on hydrogels the delivery stem cells, growth factors, or small molecules drugs. Despite ability to enhance all these methods, no material has significantly regrown vasculature within translatable timeline days weeks stroke. Here we developed 2 novel regeneration stroke, a highly porous granular hydrogel termed Cryo microgels,...
Abstract Macroporous scaffolds are being increasingly used in regenerative medicine and tissue repair. While our recently developed microporous annealed particle (MAP) have overcome issues with injectability situ hydrogel formation, limitations respect to tunability be able manipulate strength rigidity for broad applications still exist. To address these key issues, here we synthesized microparticles (HMPs) of hyaluronic acid (HA) using the thiol-norbornene click reaction then subsequently...
Abstract Date Presented 4/20/2018 The study of highlights successful strategies for young people with visual impairments to transition into living as independent adults through a cooking group intervention focused on self-determination and skills. Primary Author Speaker: Jessica Tsotsoros Additional Authors Speakers: Kelsie Burkeen, Nicole Darling, Morgan Gilsinger, Carlye Harris Contributing Authors: Lynda Crouch