An important enabling factor for realising integrated micro fluidic analysis instruments medical diagnostics purposes is front-end sample preparation. Dielectrophoresis a method that offers great potential cell discrimination and isolation processing, here we have applied it to the problem of isolating malaria-infected cells from blood. During development malarial pathogen, Plasmodium falciparum, increases occur in ionic permeability plasma membrane infected erythrocytes. When challenged by...

Glycogen synthase kinase-3β (GSK3β) is a central figure in Wnt signaling, which its activity controlled by regulatory binding proteins. Here we show that proteins outside the pathway also control of GSK3β. DNA damage induced camptothecin, activates tumor suppressor p53, was found to activate This activation occurred phosphorylation-independent mechanism involving direct GSK3β confined nucleus where p53 localized, and mutated (R175H) bound but did not Activation GSK3 promoted responses...

The recent discovery of direct interactions between two important regulators cell fate, the tumor suppressor p53 and glycogen synthase kinase-3β (GSK3β), led us to examine mechanism outcomes this interaction. Two regions were identified that regulate its binding GSK3β. Deletion activation domain-1 (AD1), but not mutations prevent MDM2 through AD1 domain, enhanced GSK3β p53, indicating domain interferes with basic (BD) abrogated binding, a ten amino acid region within C-terminal BD was as...

Abstract Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) are the two most popular surfactants among perfluorinated compounds (PFCs), with a wide range of uses. Growing evidence suggests that PFCs have potential to interfere estrogen homeostasis, posing risk endocrine‐disrupting effects. This in vitro study aimed investigate estrogenic effect these on T47D hormone‐dependent breast cancer cells. PFOS PFOA (10 −12 10 −4 M) were not able induce response element (ERE)...

Andrographis paniculata is widely used in traditional herbal medicines for the treatment of common cold, fever and diarrhea, many regions Scandinavia Asia, including Thailand. The pharmacological activities A. are mainly attributed to active diterpenoids 14-deoxyandrographolide, which uniquely high first true leaf ethanolic extract (FTLEE) paniculata. In this study, acute toxicity standardized FTLEE was examined according OECD test guideline No. 420. Mice were divided into four groups each...

Glycogen synthase kinase-3 (GSK3) and p53 play crucial roles in the mitochondrial apoptotic pathway are known to interact nucleus. However, it is not if GSK3 has a regulatory role translocation of that participates signaling following DNA damage. In this study, we demonstrated lithium SB216763, which pharmacological inhibitors GSK3, attenuated accumulation caspase-3 activation, as shown by PARP cleavage induced DNA-damaging agents doxorubicin, etoposide camptothecin. Furthermore, each these...

Cholangiocarcinoma (CCA) is a malignant tumor of the biliary epithelium associated with Opisthorchis viverrini, primary sclerosing cholangitis and hepatitis viral infection. In global population, men have higher incidence rates for CCA than women; thus, gender disparity in progression chronic inflammation duct leading to malignancy may involve effects estrogen (E2). Genistein (GE), prominent phytoestrogen found soy products, an receptor β (ERβ) agonist tyrosine kinase inhibitor. The present...

Sesame lignans (sesamin, sesamolin) and their metabolites (enterodiol, ED; enterolactone, EL; sesamol) have been evaluated for estrogenic activities. ED EL indicated to estrogenic/antiestrogenic properties on human breast cancer cells; however the activities of sesamin, sesamolin sesamol not reported. In present study, potencies sesame were determined by estrogen responsive element (ERE) luciferase reporter assay in T47D cells stably transfected with ERE-luc (T47D-KBluc cells) quantifying...

<i>Andrographis paniculata</i> is an important herbal medicine widely used in several Asian countries for the treatment of various diseases due to its broad range pharmacological activities. The present study reports that <i>A. extracts potently inhibit growth liver (HepG2 and SK-Hep1) bile duct (HuCCA-1 RMCCA-1) cancer cells. with different contents major diterpenoids, including andrographolide, 14-deoxy-11,12-didehydroandrographolide, neoandrographolide, 14-deoxyandrographolide, exhibited...

Cholangiocarcinoma (CCA) is a malignant cancer of the biliary tract and its occurrence associated with chronic cholestasis which causes an elevation bile acids in liver duct. The present study aimed to investigate role mechanistic effect on CCA cell growth. Intrahepatic lines, RMCCA-1 HuCCA-1, were treated their metabolites determine growth promoting effect. Cell viability, cycle analysis, EdU incorporation assays conducted. Intracellular signaling proteins detected by western...

Background The available in vitro evidences suggest the inherent instability and interconvertibility of [6]-gingerol [6]-shogaol. However, limited data on their vivo interconversion hinder understanding influence pharmacokinetic profiles. Purpose This study presents first comprehensive investigation aiming to determine pharmacokinetics rats, elucidate oral bioavailability, target distribution, biotransformation, excretion profiles key ginger constituents, [6]-gingerol, [6]-shogaol,...

Abstract Arsenic is an environmental toxicant that recently has been shown to have anticancer activity against a number of types cancer cells by inducing apoptosis. Glycogen synthase kinase‐3 (GSK3), serine/threonine kinase, important pro‐apoptotic signaling enzyme. Although GSK3 promote apoptosis caused wide variety insults, role for in arsenic‐induced not yet identified. Investigation the involvement arsenite‐induced demonstrated arsenite induced SH‐SY5Y human neuroblastoma cells,...

Arsenic (As) is considered a major environmental health threat worldwide due to its widespread contamination in drinking water. Recent studies reported that arsenic potential xenoestrogen as it interfered with the action of estrogen (E2) and receptor (ER) signaling. The present study investigated effects sodium arsenite (NaAsO2) on signaling human breast cancer cells. results demonstrated NaAsO2 dose-dependently increased viability hormone-dependent MCF-7 T47D cells expressing both ERα ERβ...

Acrylonitrile (ACN) is a chemical that widely used in the production of plastics, acrylic fibers, synthetic rubbers and resins. It has been reported ACN can cause oxidative stress, condition which well recognized as an apoptotic initiator; however, information regarding ACN-induced apoptosis limited. This present study investigated whether induces human neuroblastoma SH-SY5Y cells, its induction involves stress. The results showed caused activation caspase-3, key enzyme involved apoptosis,...

Arsenic is a widespread contaminant in the environment especially drinking water. Although it known carcinogen human, mechanism by which arsenic induces carcinogenesis not well understood. Among several effects of arsenic, has been suggested that arsenic-induced vascular endothelial growth factor (VEGF) expression plays critical role carcinogenesis. In present study, we demonstrated arsenite induced VEGF neuroblastoma SH-SY5Y cells without induction HIF-1α, well-known transcriptional...

This study was undertaken to determine levels of contamination toxic metals in water, sediment, and consumed fishery products from Phayao freshwater lake located northern Thailand, which is a major water resource for drinking agriculture, household use. Concentrations Mn, As, other were determined fish tissues (Puntius gonionotus) pond snails (Filopaludina martensi). Sampling carried out 3 periods (February, May, August) 2005. Metal analysis performed by using inductively coupled plasma mass...