A5018575489- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Developmental Biology and Gene Regulation
- Single-cell and spatial transcriptomics
- Neurobiology and Insect Physiology Research
- Cell Image Analysis Techniques
- Cellular Mechanics and Interactions
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Marine Biology and Environmental Chemistry
- Gene Regulatory Network Analysis
- Sustainable Building Design and Assessment
- Morphological variations and asymmetry
- Invertebrate Immune Response Mechanisms
- RNA modifications and cancer
- Hippo pathway signaling and YAP/TAZ
- Building Energy and Comfort Optimization
- Facilities and Workplace Management
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Photosynthetic Processes and Mechanisms
- Advanced Chemical Sensor Technologies
- 3D Printing in Biomedical Research
- Congenital heart defects research
- Silk-based biomaterials and applications
California University of Pennsylvania
2025
University of Pennsylvania
2011-2024
UNSW Sydney
2017-2020
Princeton University
2011-2018
Institute for Integrative and Experimental Genomics
2016
National University of Singapore
1978-1984
Zygotic genome activation (ZGA) is a major programming event whereby the cells of embryo begin to adopt specified fates. Experiments in Drosophila and zebrafish have revealed that ZGA depends on transcription factors provide large-scale control gene expression by direct specific binding regulatory sequences [1Liang H.L. Nien C.Y. Liu H.Y. Metzstein M.M. Kirov N. Rushlow C. The zinc-finger protein Zelda key activator early zygotic Drosophila.Nature. 2008; 456: 400-403Crossref PubMed Scopus...
Significance Classical studies of gene activity in development focused on spatial limits due to the use fixed tissues for analysis. The advent live-imaging methods provides an opportunity examine temporal control expression. Here, we used quantitative visualize dynamic shifts endogenous expression eve and ftz pair-rule stripes early Drosophila embryo. We suggest that these dynamics add complexity encoded by segmentation network.
ERK controls gene expression in development, but mechanisms that link activation to changes transcription are not well understood. We used high-resolution analysis of signaling dynamics study transcriptional interpretation during Drosophila embryogenesis, at a stage when induces intermediate neuroblasts defective ( ind ), essential for patterning the nerve cord. by antagonizing its repression Capicua (Cic), factor acts as sensor receptor tyrosine kinases animal development and human...
Significance At the mid-blastula transition, rapid cell divisions are slowed down and global activation of zygotic genes occur, preparing early embryo for differentiation further development. Using live imaging embryos with reduced ploidy cycle lengths, we examined effects nuclear content to cytoplasm (N/C) ratio on timing genome activation. While some predominantly affected by N/C ratio–dependent changes in durations, other demonstrate direct regulation through transcriptional kinetics...
While it is well acknowledged that specific enhancer-promoter interactions are essential for transcription, the spatiotemporal thresholds required transcription initiation remain unclear. Here, we employed phenomenon of transvection, where an enhancer activates target gene in trans at homologous position, to analyze mechanism long-range interactions. We combined MS2- and PP7-based RNA labeling with ParB/parS DNA simultaneously visualize active a locus living Drosophila embryos. By...
The LIF signaling pathway and its regulation of internal factors like Sox2 is crucial for maintaining self-renewal pluripotency in mESCs. However, the direct impact on transcriptional dynamics at single-cell level remains elusive. Here, we employ PP7/PCP-mediated live imaging to analyze under perturbation resolution. Removal ligand or addition a JAK inhibitor heterogeneously affects cell population, reducing number Sox2-active cells, rather than completely abolishing expression. Moreover,...
The regulatory specificity of a gene is determined by the structure its enhancers, which contain multiple transcription factor binding sites. A unique combination sites in an enhancer determines boundary target expression, and their disruption often leads to developmental defects. Despite extensive characterization motifs enhancer, it still unclear how each site contributes overall transcriptional activity. Using live imaging, quantitative analysis, mathematical modeling, we measured...
The dorsoventral (DV) axis of the Drosophila embryo is patterned by a nuclear gradient Rel family transcription factor, Dorsal (Dl), that activates or represses numerous target genes in region-specific manner. Here, we demonstrate signaling receptor tyrosine kinases (RTK) reduces levels and transcriptional activity Dl, both at poles mid-body embryo. These effects depend on wntD, which encodes Dl antagonist belonging to Wingless/Wnt secreted factors. Specifically, show that, via relief...
Calcium-dependent cysteine proteases of the calpain family are modulatory that cleave their substrates in a limited manner. Among substrates, calpains target vertebrate and invertebrate IκB proteins. Because proteolysis by potentially generates novel protein functions, it is important to understand how this affects NFκB activity. We investigate action Calpain A (CalpA) on Drosophila melanogaster homologue Cactus vivo. CalpA alters absolute amounts protein. Our data indicate, however, uses...
Interest in Corporate Social Responsibility (CSR) is growing the construction sector. In response to societal changes, professionals and businesses they work are expected contribute positively environments communities which build. Hitherto, it appears that there has been little research mapping out CSR focuses initiatives of engineering firms. addressing this gap, 104 from across supply chain Australia New Zealand was undertaken. The overall results reveal many firms' mainly on occupational...
Progress of development is commonly reconstructed from imaging snapshots chemical or mechanical processes in fixed tissues. As a first step these reconstructions, must be spatially registered and ordered time. Currently, image registration ordering are often done manually, requiring significant amount expertise with specific system. However, as the sizes data sets grow, tasks become increasingly difficult, especially when images noisy developmental changes being examined subtle. To address...