A5037733924- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Vagus Nerve Stimulation Research
- EEG and Brain-Computer Interfaces
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Iron Metabolism and Disorders
- Epilepsy research and treatment
- Heart Rate Variability and Autonomic Control
- Transcranial Magnetic Stimulation Studies
- Hemoglobinopathies and Related Disorders
- Blind Source Separation Techniques
- Receptor Mechanisms and Signaling
- Photochromic and Fluorescence Chemistry
- Nicotinic Acetylcholine Receptors Study
- Lipid metabolism and disorders
- Pharmacological Effects and Toxicity Studies
- Neuroscience of respiration and sleep
- Mitochondrial Function and Pathology
- Sleep and Wakefulness Research
- Adenosine and Purinergic Signaling
- Dialysis and Renal Disease Management
- Neurological disorders and treatments
- Cancer, Lipids, and Metabolism
- Lipid metabolism and biosynthesis
Ghent University
2014-2025
Ghent University Hospital
2014-2024
University of Groningen
2021
University Medical Center Groningen
2021
Amsterdam University Medical Centers
2019-2021
Amsterdam UMC Location University of Amsterdam
2020
University Medical Center
2019
University Hospital and Clinics
2019
University of Pennsylvania
2019
Amsterdam UMC Location Vrije Universiteit Amsterdam
2019
The intrahippocampal kainic acid (IHKA) mouse model is an extensively used in vivo to investigate the pathophysiology of mesial temporal lobe epilepsy (mTLE) and develop novel therapies for drug-resistant epilepsy. It characterized by profound hippocampal sclerosis spontaneously occurring seizures with a major role injected damaged hippocampus, but little known about excitability specific subregions. purpose this study was electrophysiologically characterize subregions chronic phase induced...
Abstract Objective More than one‐third of patients with temporal lobe epilepsy (TLE) continue to have seizures despite treatment antiepileptic drugs, and many experience severe drug‐related side effects, illustrating the need for novel therapies. Selective expression inhibitory Designer Receptors Exclusively Activated by Drugs (DREADDs) allows cell‐type‐specific reduction neuronal excitability. In this study, we evaluated effect chemogenetic suppression excitatory pyramidal granule cell...
Up to 30 % of epilepsy patients suffer from drug-resistant (DRE). The search for innovative therapies is therefore important close the existing treatment gap in these patients. adenosinergic system possesses potent anticonvulsive effects, mainly through adenosine A1 receptor (A1R). However, clinical application A1R agonists hindered by severe systemic side effects. To achieve local modulation A1Rs, we employed a photopharmacological approach using caged version agonist...
Abstract. Gastrointestinal iron absorption has been measured in 27 apparently healthy subjects (8 males and 19 females) by means of whole body counting. Whole retention 14 days after oral administration 10 μCi 59 Fe with a carrier dose 9.9 mg 2+ was used as an expression absorption. The percentage incorporation the orally applied into total erythrocyte mass within (erythrocyte incorporation) estimated well. Geometric mean 9.1 ± 2.2 (S.D.) % 8.3 2.1% females; entire series it 8.5 2.1%....
The importance of protein glycosylation in regulating lipid metabolism is becoming increasingly apparent. We set out to further investigate this by studying patients with type I congenital disorders (CDGs) defective N-glycosylation.We studied 29 the 2 most prevalent types CDG, ALG6 (asparagine-linked 6)-deficiency CDG and PMM2 (phosphomannomutase 2)-deficiency 23 first- second-degree relatives a heterozygous mutation measured plasma cholesterol levels. Low-density lipoprotein (LDL) was 3...
Summary Objective One third of epilepsy patients do not become seizure‐free using conventional medication. Therefore, there is a need for alternative treatments. Preclinical research designer receptors exclusively activated by drugs (DREADDs) has demonstrated initial success in suppressing epileptic activity. Here, we evaluated whether long‐term chemogenetic seizure suppression could be obtained the intraperitoneal kainic acid rat model temporal lobe epilepsy, when DREADDs were selectively...
The locus coeruleus (LC)-noradrenaline (NA) system plays a crucial role in modulating neuronal excitability and plasticity. In epilepsy, the LC-NA an important regulating seizure thresholds severity, with elevated NA release mediating seizure-suppressing effects of vagus nerve stimulation (VNS). We investigated whether chemogenetic LC activation is able to increase hippocampal affect electrophysiology anesthetized rats. 22 male Sprague-Dawley rats were injected viral vector AAV9-hSyn-NE2m...
Epilepsy is a neurological disorder characterized by recurrent epileptic seizures. Electrophysiological and neuroimaging studies in patients with epilepsy suggest that abnormal functional brain networks play role the development of epilepsy, i.e. epileptogenesis, resulting generation spontaneous seizures cognitive impairment. In this longitudinal study, we investigated changes during epileptogenesis intraperitoneal kainic acid (IPKA) rat model temporal lobe (TLE) using resting state magnetic...
Epilepsy is a neurological disorder characterized by recurrent epileptic seizures. The involvement of abnormal functional brain networks in the development epilepsy and its comorbidities has been demonstrated electrophysiological neuroimaging studies patients with epilepsy. This longitudinal study investigated changes dynamic connectivity (dFC) network topology during using intraperitoneal kainic acid (IPKA) rat model temporal lobe (TLE). Resting state magnetic resonance images (rsfMRI) 20...
Selective neuromodulation using designer receptors exclusively activated by drugs (DREADDs) has become an increasingly important research tool, as well emerging therapeutic approach. However, the safety profile of DREADD expression is unknown. Here, different titers adeno-associated viral (AAV) vector were administered in attempt to vary total levels inhibitory hM4D(Gi) excitatory hippocampal neurons. Male Sprague Dawley rats injected with AAV2/7 encoding DREADD-mCherry, DREADD, or mCherry....
The locus coeruleus (LC) is a small brainstem nucleus and the sole source of noradrenaline in neocortex, hippocampus cerebellum. Noradrenaline powerful neuromodulator involved regulation excitability plasticity large-scale brain networks. In this study, we performed detailed assessment activity neurons changes noradrenergic transmission during acute hippocampal seizures evoked with perforant path stimulation, using state-of-the-art methodology. Action potentials LC neurons, which some were...
Recently, novel inborn errors of metabolism were identified because mutations in V-ATPase assembly factors TMEM199 and CCDC115. Patients are characterized by generalized protein glycosylation defects, hypercholesterolemia, fatty liver disease. Here, we set out to characterize the lipid phenotype human plasma, cell models, a mouse model.Patients with CCDC115 displayed hyperlipidemia, increased levels lipoproteins very low density lipoprotein range. HepG2 hepatoma cells, which expression was...
Abstract Adenosine is a potent regulator of neurotransmission and neuronal excitability through activation G i protein-coupled adenosine A 1 receptors (A Rs). It has gained interest as potential anticonvulsant due to its endogenous involvement in ending ongoing seizure activity. recently developed coumarin-caged derivative the R agonist N 6 -cyclopentyl-adenosine (CPA), cCPA, was used for photo-uncaging CPA with millisecond flashes 405 nm light. At population level, reduces Schaffer...
Abstract Objective. The blue light-activated inhibitory opsin, stGtACR2, is gaining prominence as a neuromodulatory tool due its ability to shunt-inhibit neurons and being frequently used in vivo experimentation. However, experiments involving stGtACR2 use longer durations of light pulses, which inadvertently heat up the local brain tissue confound experimental results. Therefore, heating effects illumination parameters for optogenetic inhibition must be evaluated. Approach. To assess (473...