A5002026143- Biochemical and Molecular Research
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Parkinson's Disease Mechanisms and Treatments
- Tuberculosis Research and Epidemiology
- Demographic Trends and Gender Preferences
- Lysosomal Storage Disorders Research
- Alzheimer's disease research and treatments
- Reproductive Health and Contraception
- Global Maternal and Child Health
- Legume Nitrogen Fixing Symbiosis
- Microbial Community Ecology and Physiology
- Trypanosoma species research and implications
- Endoplasmic Reticulum Stress and Disease
- Connective tissue disorders research
- Biofuel production and bioconversion
- Vibrio bacteria research studies
- Enzyme Structure and Function
- Cellular transport and secretion
- Enzyme Production and Characterization
- Intimate Partner and Family Violence
- Protist diversity and phylogeny
- Genetic Neurodegenerative Diseases
- Child Abuse and Trauma
- Kidney Stones and Urolithiasis Treatments
Universidade Nova de Lisboa
2007-2020
Instituto de Biologia Experimental e Tecnológica
2015
Abstract Phosphatidylinositol is critical for intracellular signalling and anchoring of carbohydrates proteins to outer cellular membranes. The defining step in phosphatidylinositol biosynthesis catalysed by CDP-alcohol phosphotransferases, transmembrane enzymes that use CDP-diacylglycerol as donor substrate this reaction, either inositol eukaryotes or phosphate prokaryotes the acceptor alcohol. Here we report structures a related enzyme, phosphatidylinositol-phosphate synthase from...
The intracellular accumulation of low molecular mass organic compounds in response to stressful conditions was investigated the thermophilic bacterium Petrotoga miotherma , a member order Thermotogales . This led discovery new solute, whose structure established as α‐ d ‐mannopyranosyl‐(1→2)‐α‐ ‐glucopyranosyl‐(1→2)‐glycerate (MGG) by MMR spectroscopy and MS. Under optimum growth (3% NaCl; 55 °C), MGG major solute [up 0.6 µmol·(mg protein) −1 ]; α‐glutamate proline were also present but...
The compatible solute mannosylglucosylglycerate (MGG), recently identified in Petrotoga miotherma, also accumulates mobilis response to hyperosmotic conditions and supraoptimal growth temperatures. Two functionally connected genes encoding a glucosyl-3-phosphoglycerate synthase (GpgS) an unknown glycosyltransferase (gene Pmob_1143), which we characterized as mannosylglucosyl-3-phosphoglycerate designated MggA, were the genome of Ptg. mobilis. This enzyme used product GpgS, (GPG), well...
We describe a novel biosynthetic pathway for glycerophosphoinositides in Rhodothermus marinus which inositol is activated by cytidine triphosphate (CTP); this unlike all known pathways that involve activation of the lipid group instead. This work was motivated detection R. genome gene with high similarity to CTP:L-myo-inositol-1-phosphate cytidylyltransferase, enzyme synthesizes diphosphate (CDP)-inositol, metabolite only synthesis di-myo-inositol phosphate. However, solute absent marinus....
Trehalose uptake at 65 degrees C in Rhodothermus marinus was characterized. The profile of trehalose as a function concentration showed two distinct types saturation kinetics, and the analysis data complicated by activity periplasmic trehalase. kinetic parameters this enzyme determined whole cells were follows: Km = 156 +/- 11 microM Vmax 21.2 0.4 nmol/min/mg total protein. Therefore, could be acted upon activity, yielding glucose that subsequently entered cell via system, which also To...