A5033418156- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Escherichia coli research studies
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Protist diversity and phylogeny
- Biochemical and Structural Characterization
- CRISPR and Genetic Engineering
- Vibrio bacteria research studies
- Plant and Biological Electrophysiology Studies
Massachusetts Institute of Technology
2020-2021
Laboratoire de Chimie Bactérienne
2012-2018
Centre National de la Recherche Scientifique
2012-2017
Aix-Marseille Université
2015-2017
Institut de Microbiologie de la Méditerranée
2012-2017
In Myxococcus xanthus the gliding motility machinery is assembled at leading cell pole to form focal adhesions, translocated rearward propel cell, and disassembled lagging pole. We show that MglA, a Ras-like small G-protein, an integral part of this machinery. function, MglA stimulates assembly complex by directly connecting it MreB actin cytoskeleton. Because nucleotide state regulated spatially only binds in guanosine triphosphate–bound form, complexes are dispersed where triphosphatase...
Migrating cells employ sophisticated signal transduction systems to respond their environment and polarize towards attractant sources. Bacterial also regulate polarity dynamically reverse direction of movement. In Myxococcus xanthus, a GTP-bound Ras-like G-protein, MglA, activates the motility machineries at leading cell pole. Reversals are provoked by pole-to-pole switching which is under control chemosensory-like cascade (Frz). It was previously known that asymmetric localization MglA one...
Understanding the principles underlying plasticity of signal transduction networks is fundamental to decipher functioning living cells. In Myxococcus xanthus, a particular chemosensory system (Frz) coordinates activity two separate motility systems (the A- and S-motility systems), promoting multicellular development. This unusual structure asks how transduced in branched pathway. Using combined evolution-guided single cell approaches, we successfully uncoupled regulations showed that...
Caulobacter crescentus is a major model organism for understanding cell cycle regulation and cellular asymmetry. The current genetic tools deleting or silencing the expression of individual genes, particularly those essential viability, are time-consuming labor-intensive, which limits global studies. Here, we optimized CRISPR interference (CRISPRi) use in . Using Streptococcus thermophilus CRISPR3 pasteurianus systems, show that coexpression catalytically dead form Cas9 (dCas9) with single...
Abstract In every organism, the cell cycle requires execution of multiple cellular processes in a strictly defined order. However, mechanisms used to ensure such order remain poorly understood, particularly bacteria. Here, we show that activation essential CtrA signaling pathway triggers division Caulobacter crescentus is intrinsically coupled successful initiation DNA replication via physical translocation newly-replicated chromosome, powered by ParABS system. We demonstrate ParA...
Summary Dynamic control of cell polarity is critical importance for many aspects cellular development and motility. In Myxococcus xanthus , a G-protein its cognate GTPase-activating protein establish axis that defines the direction movement which can be rapidly inverted by Frz chemosensory system. Although vital collective behaviours, how triggers this switch has remained unknown. Here, we use genetics, imaging mathematical modelling to show controls reversals via gated relaxation...