A5091430435- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neural Engineering
- Genetics and Neurodevelopmental Disorders
- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Cerebrovascular and genetic disorders
- Genetic Neurodegenerative Diseases
- Erythrocyte Function and Pathophysiology
- Microfluidic and Capillary Electrophoresis Applications
- Thyroid Disorders and Treatments
- Connective tissue disorders research
- Genomics and Rare Diseases
- Cellular transport and secretion
- Growth Hormone and Insulin-like Growth Factors
- Parkinson's Disease Mechanisms and Treatments
- CRISPR and Genetic Engineering
- Genomic variations and chromosomal abnormalities
- Electrowetting and Microfluidic Technologies
- Mitochondrial Function and Pathology
- Tissue Engineering and Regenerative Medicine
- Nuclear Receptors and Signaling
Technische Universität Braunschweig
2022-2025
University of Lübeck
2016-2022
The hypothalamic-pituitary-thyroid (HPT) axis maintains circulating thyroid hormone levels in a narrow physiological range. As axons containing thyrotropin-releasing (TRH) terminate on hypothalamic tanycytes, these specialized glial cells have been suggested to influence the activity of HPT axis, but their exact role remained enigmatic. Here, we demonstrate that stimulation TRH receptor 1 increases intracellular calcium tanycytes median eminence via Gαq/11 proteins. Activation pathways size...
Two-phase biocatalysis in batch reactions often suffers from inefficient mass transfer, inconsistent reaction conditions, and enzyme inactivation issues. Microfluidics offer uniform controlled environments ensuring better reproducibility enable efficient, parallel processing of many small-scale reactions, making more scalable. In particular, the use microfluidic droplets can increase interfacial area between two phases therefore also rates. For these reasons, slug flow has been extensively...
Direct reprogramming from somatic to neural cell types has become an alternative induced pluripotent stem cells. Most protocols employ viral expression systems, posing the risk of random genomic integration. Recent developments led plasmid-based protocols, lowering this risk. However, these either relied on continuous presence a variety small molecules or were only able reprogram murine We therefore established protocol based vectors containing Epstein-Barr virus (EBV)-derived oriP/EBNA1 as...
Keeping neural stem cells under proliferation, followed by terminal differentiation, can substantially increase the number of neurons generated. With regard to usability proliferating neurospheres (NSPHs) cultures, adherent induction protocols have not yet been studied in comparison embryoid body (EB)-based protocols. To compare these proctocols, human induced pluripotent was performed dual SMAD inhibition both and free-floating EB culture conditions. After 10 days, we transferred...
Mutations in TUBB4A have been identified to cause a wide phenotypic spectrum of diseases ranging from hereditary generalized dystonia with whispering dysphonia (DYT-TUBB4A) and spastic paraplegia (HSP) leukodystrophy hypomyelination atrophy the basal ganglia cerebellum (H-ABC). encodes brain-specific β-tubulin isotype, 4A. To elucidate pathogenic mechanisms conferred by mutations leading different phenotypes, we functionally characterized three variants (c.4C>G,p.R2G; c.745G>A,p.D249N;...
Mutations in the brain-specific β-tubulin 4A (TUBB4A) gene cause a broad spectrum of diseases, ranging from dystonia (DYT-TUBB4A) to hypomyelination with atrophy basal ganglia and cerebellum (H-ABC). Currently, mechanisms how TUBB4A variants lead this pleiotropic manifestation remain elusive. Here, we investigated whether mutations causing either DYT-TUBB4A (p.R2G p.Q424H) or H-ABC (p.R2W p.D249N) exhibit differential effects at molecular cellular levels. Using live-cell imaging...
Impairment of the dopaminergic (DA) system is a common cause several movement disorders including Parkinson's disease (PD), however, little known about underlying mechanisms. The recent development stem-cell-based protocols for generation DA neurons partially solved this issue, technology costly and time-consuming. Commonly used cell lines, i.e. neuroblastoma (SHSY5Y) PC12 cells are still widely to investigate PD significantly contributed our understanding mechanisms involved in disease....
Embedded porous membranes are a key element of various organ-on-chip systems. The widely used commercial polymer impose limits with regard to chip integration and thinness. We report microfluidic platform the monolithically integrated, ultra-thin (700 nm) nanoporous membrane made ultra-low-stress ( <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="m1"><mml:mo><</mml:mo></mml:math> 35 MPa) Si x N y for culturing testing reconstructed tissue. is designed support in vitro tissues...
The differentiation of human induced pluripotent stem cells (hiPSCs) into specific cell types for disease modeling and restorative therapies is a key research agenda offers the possibility to obtain patient-specific interest wide range diseases. Basal forebrain cholinergic neurons (BFCNs) play particular role in pathophysiology Alzheimer’s dementia isolated dystonias. In this work, various directed protocols based on monolayer neural induction were tested their effectiveness promoting...