A5029708233- Genetics, Aging, and Longevity in Model Organisms
- Viral Infectious Diseases and Gene Expression in Insects
- Receptor Mechanisms and Signaling
- CRISPR and Genetic Engineering
- Heat shock proteins research
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Gene Regulatory Network Analysis
- Spaceflight effects on biology
- DNA Repair Mechanisms
- Estrogen and related hormone effects
- CAR-T cell therapy research
- Mitochondrial Function and Pathology
- Reproductive System and Pregnancy
- Genetics and Neurodevelopmental Disorders
- DNA and Nucleic Acid Chemistry
- Cellular Mechanics and Interactions
- Bacteriophages and microbial interactions
- Genetic Neurodegenerative Diseases
- RNA and protein synthesis mechanisms
AstraZeneca (Sweden)
2018-2023
University of Gothenburg
2010-2021
G protein-coupled receptor (GPCR) signaling is the primary method eukaryotes use to respond specific cues in their environment. However, relationship between stimulus and response for each GPCR difficult predict due diversity natural signal transduction architecture expression. Using genome engineering yeast, we constructed an insulated, modular system study how stimuli can be predictably tuned using synthetic tools. We delineated contributions of a minimal set key components via...
70 kDa heat shock proteins (Hsp70) are essential chaperones of the protein quality control network; vital for cellular fitness and longevity. The four cytosolic Hsp70’s in yeast, Ssa1-4, thought to be functionally redundant but absence Ssa1 Ssa2 causes a severe reduction reproduction accelerates replicative aging. In our efforts identify which Hsp70 activities most important longevity assurance, we systematically investigated capacity Ssa4 carry out different performed by Ssa1/2...
Site saturation mutagenesis (SSM) is a powerful strategy for protein engineering and directed evolution experiments. However, limiting factors using this method are either biased representation of variants, or library size. To overcome these hurdles, we generated large scale targeted synthetic SSM libraries massively parallel oligonucleotide synthesis benchmarked against an error-prone (epPCR) library. The yeast glucose activated GPCR—Gpr1 was chosen as prototype to evolve novel sensors. We...
Ubp3 is a conserved ubiquitin protease that acts as an antisilencing factor in MAT and telomeric regions. Here we show ubp3∆ mutants also display increased silencing ribosomal DNA (rDNA). Consistent with this, RNA polymerase II occupancy lower cells lacking than wild-type all heterochromatic Moreover, mutant, unequal recombination rDNA highly suppressed. We present genetic evidence this effect on recombination, but not silencing, entirely dependent the Sir2. Further, sir2∆ age prematurely at...
Rapid and flexible plasmid construct generation at scale is one of the most limiting first steps in drug discovery projects. These hurdles can partly be overcome by adopting modular DNA design principles, automated sequence fragmentation, assembly. To this end we have designed a robust, multimodule golden gate based cloning platform for with wide range applications. The assembly efficiency system was validated splitting sfGFP sfCherry3C cassettes expressing them E. coli followed fluorometric...
Abstract G protein-coupled receptor (GPCR) signaling is the primary method eukaryotes use to respond specific cues in their environment. However, relationship between stimulus and response for each GPCR difficult predict due diversity natural signal transduction architecture expression. Using genome engineering yeast, we here constructed an insulated, modular system study how stimuli can be predictably tuned using synthetic tools. We delineated contributions of a minimal set key components...
ABSTRACT 70 kDa heat shock proteins (Hsp70) are essential chaperones of the protein quality control network; vital for cellular fitness and longevity. The four cytosolic Hsp70’s in yeast, Ssa1-4, thought to be functionally redundant but absence Ssa1 Ssa2 causes a severe reduction reproduction accelerates replicative aging. In our efforts identify which Hsp70 activities most important longevity assurance, we systematically investigated capacity Ssa4 carry out different performed by Ssa1/2...