A5080862200- Parkinson's Disease Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Neurological disorders and treatments
- Genetic Neurodegenerative Diseases
- Pluripotent Stem Cells Research
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Neuroscience and Neural Engineering
- Occupational and environmental lung diseases
- Graphene and Nanomaterials Applications
- Organic Electronics and Photovoltaics
- Ginkgo biloba and Cashew Applications
- Neuroblastoma Research and Treatments
- Autophagy in Disease and Therapy
- interferon and immune responses
- Bat Biology and Ecology Studies
- Gene Regulatory Network Analysis
- Neurogenesis and neuroplasticity mechanisms
- Tuberculosis Research and Epidemiology
- Bacterial Infections and Vaccines
- Cell Image Analysis Techniques
- Conducting polymers and applications
- Neuroscience and Neuropharmacology Research
- Child Nutrition and Feeding Issues
- RNA and protein synthesis mechanisms
Nazarbayev University
2020
University of Luxembourg
2011-2020
Centre Hospitalier de Luxembourg
2016
University of Florida
2007
Institute of Cell Biophysics
2004
Institute of Theoretical and Experimental Biophysics
2004
The immortalized and proliferative cell line SH-SY5Y is one of the most commonly used lines in neuroscience neuroblastoma research. However, undifferentiated cells share few properties with mature neurons. In this study, we present an optimized neuronal differentiation protocol for that requires only two work steps 6 days. After differentiation, increased levels ATP plasma membrane activity but reduced expression energetic stress response genes. Differentiation results mitochondrial...
Abstract The secretion of biomolecules into the extracellular milieu is a common and well‐conserved phenomenon in biology. In bacteria, secreted are not only involved intra‐species communication but they also play roles inter‐kingdom exchanges pathogenicity. To date, released products, such as small molecules, DNA , peptides, proteins, have been well studied bacteria. However, bacterial RNA complement has so far comprehensively characterized. Here, we analyzed, using combination physical...
Differentiation of hNESC into functional dopaminergic neurons in phase-guided 3D microfluidic cell culture.
Differentiated cells can be converted directly into multipotent neural stem (i.e., induced [iNSCs]). iNSCs offer an attractive alternative to pluripotent cell (iPSC) technology with regard regenerative therapies. Here, we show in vivo long-term analysis of transplanted the adult mouse brain. showed sound survival rates without graft overgrowths. The displayed a multilineage potential clear bias toward astrocytes and permanent downregulation progenitor cell-cycle markers, indicating that are...
The gut is proposed as a starting point of idiopathic IPD, but the presence α-synuclein in IPD colon mucosa debated.The objective this study was to evaluate if can serve biomarker IPD.Immunohistochemistry used locate and quantify blinded approach from biopsies right left 19 patients 8 controls.Total present all 1 controls; phosphorylated subjects. There no intensity difference depending on disease status. Staining total stronger (p = .04).Conventional immunohistochemistry staining mucosal...
Abstract Mitochondrial dysfunction is a hallmark in idiopathic Parkinson’s disease (IPD). Here, we established screenable phenotypes of mitochondrial morphology and function primary fibroblasts derived from patients with IPD. Upper arm punch skin biopsy was performed 41 mid-stage IPD 21 age-matched healthy controls. At the single-cell level, basal membrane potential (Ψm) higher than Similarly, under carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) stress, remaining Ψm increased...
Biological systems present multiple scales of complexity, ranging from molecules to entire populations. Light microscopy is one the least invasive techniques used access information various biological in living cells. The combination molecular biology and imaging provides a bottom-up tool for direct insight into how processes work on cellular scale. However, can also be as top-down approach study behavior system without detailed prior knowledge about its underlying mechanisms. In this...
Abstract Based on autopsy material mitochondrial dysfunction has been proposed being part of the pathophysiological cascade Parkinson’s disease (PD). However, in living patients, evidence for such is scarce. As presumably starts at enteric level, we studied ganglionic and morphometrics neurons. We compared 65 ganglia from 11 PD patients without intestinal symptoms 41 4 age-matched control subjects. found that colon had smaller volume, contained significantly more mitochondria per ganglion...
Bronchial airway epithelial cells (BAEpC) are among the first to encounter M. tuberculosis following airborne infection. However, response of BAEpC infection has been little studied. This study investigates a human cell line (BEAS-2B) with Mycobacterium bovis Bacille Calmette Guerin (BCG). Cultured BEAS-2B were experimentally infected BCG. Uninfected cultures included as controls. Following infection, evaluated by various methods at time points up 3 days. Cell proliferation was cellular...
Mitochondrial dysfunction is a hallmark of idiopathic Parkinson's disease (IPD), which has been reported not to be restricted striatal neurons. However, studies that analyzed mitochondrial function at the level selected enzymatic activities in peripheral tissues have produced conflicting data. We considered electron transport chain as complex system with membrane potential an integrative indicator for fitness.Twenty-five IPD patients (nine females; mean duration, 6.2 years) and 16 healthy...
Abstract Mitochondrial dysfunction is linked to pathogenesis of Parkinson’s disease (PD). However, individual mitochondria-based analyses do not show a uniform feature in PD patients. Since mitochondria interact with each other, we hypothesize that PD-related features might exist topological patterns interaction networks (MINs). Here MINs formed nonclassical scale-free supernetworks colonic ganglia both from healthy controls and patients; however, altered network were observed These highly...
SUMMARY Mitochondrial dysfunction is linked to pathogenesis of Parkinson’s disease (PD). However, individual-mitochondria-based analyses do not show a uniform feature in PD patients. Since mitochondria interact with each other, we hypothesize that PD-related features might exist topological patterns mitochondria-mitochondria interaction networks (MINs). Here showed MINs form non-classical scale-free supernetworks colonic ganglia both from healthy controls and patients, however, altered are...