A5057582743- Inflammasome and immune disorders
- interferon and immune responses
- Vibrio bacteria research studies
- Immune Response and Inflammation
- Escherichia coli research studies
- Biomedical Research and Pathophysiology
- Phagocytosis and Immune Regulation
- Heme Oxygenase-1 and Carbon Monoxide
- Bacteriophages and microbial interactions
- Burkholderia infections and melioidosis
- Salmonella and Campylobacter epidemiology
- Streptococcal Infections and Treatments
- Bacterial biofilms and quorum sensing
- Cellular transport and secretion
- Peptidase Inhibition and Analysis
- Cell death mechanisms and regulation
- Legionella and Acanthamoeba research
- Immune responses and vaccinations
- Genetics, Bioinformatics, and Biomedical Research
- Antimicrobial Peptides and Activities
- Kawasaki Disease and Coronary Complications
- Lipid Membrane Structure and Behavior
- Influenza Virus Research Studies
- Mitochondrial Function and Pathology
- Glycosylation and Glycoproteins Research
University of Lausanne
2018-2024
University of Basel
2017-2019
Institut Pasteur
2012-2017
Universidade do Porto
2011-2015
Fine-tuning pyroptosis with ESCRT-III Pyroptosis is an inflammatory form of cell death induced by select caspases downstream inflammasome complexes. These cleave gasdermin D (GSDMD), whose N-terminal fragments quickly large permeability pores that induce death. However, a percentage cells active inflammasomes are resistant to pyroptosis. Rühl et al. found the membrane-remodeling machinery was recruited plasma membrane upon GSDMD activation. ESCRT-III–dependent repair limited proinflammatory...
Abstract The human non-canonical inflammasome controls caspase-4 activation and gasdermin-D-dependent pyroptosis in response to cytosolic bacterial lipopolysaccharide (LPS). Since LPS binds oligomerizes caspase-4, the pathway is thought proceed without dedicated sensors or an platform. Here we report that interferon-induced guanylate-binding proteins (GBPs) are required for by Salmonella upon delivery of LPS. GBP1 associates with surface seconds after escape from their vacuole, initiating...
Abstract Eukaryotic cells can undergo different forms of programmed cell death, many which culminate in plasma membrane rupture as the defining terminal event 1–7 . Plasma was long thought to be driven by osmotic pressure, but it has recently been shown cases an active process, mediated protein ninjurin-1 8 (NINJ1). Here we resolve structure NINJ1 and mechanism ruptures membranes. Super-resolution microscopy reveals that clusters into structurally diverse assemblies membranes dying cells,...
Abstract Ferroptosis is a regulated form of necrotic cell death caused by iron-dependent accumulation oxidized phospholipids in cellular membranes, culminating plasma membrane rupture (PMR) and lysis. PMR also hallmark other types programmed necrosis, such as pyroptosis necroptosis, where it initiated dedicated pore-forming death-executing factors. However, whether ferroptosis-associated actively executed proteins or driven osmotic pressure remains unknown. Here, we investigate potential...
Macrophages have been shown to kill Mycobacterium tuberculosis through the action of antimicrobial peptide cathelicidin (CAMP), whose expression was be induced by 1,25-dihydroxyvitamin D3 (1,25D3). Here, we investigated in detail antimycobacterial effect murine and human against smegmatis M. bovis BCG infections. We synthesized novel LL-37 variants that exhibited potent vitro bactericidal activity smegmatis, H37Rv, as compared with parental peptide. show exogenous addition or endogenous...
Targeted and specific induction of cell death in an individual or groups cells hold the potential for new insights into response tissues organisms to different forms death. Here, we report development optogenetically controlled effectors (optoCDEs), a novel class optogenetic tools that enables light-mediated three types programmed (PCD)—apoptosis, pyroptosis, necroptosis—using Arabidopsis thaliana photosensitive protein Cryptochrome-2. OptoCDEs enable rapid highly PCD human, mouse, zebrafish...
Salmonella invades epithelial cells and survives within a membrane-bound compartment, the Salmonella-containing vacuole (SCV). We isolated determined host protein composition of SCV at 30 min 3 h infection to identify characterize novel regulators intracellular bacterial localization growth. Quantitation content revealed 392 proteins specifically enriched SCVs, out which 173 associated exclusively with early 124 maturing 95 during both time-points. Vacuole interactions endoplasmic...
Salmonella enterica induces membrane ruffling and genesis of macropinosomes during its interactions with epithelial cells. This is achieved through the type three secretion system-1, which first mediates bacterial attachment to host cells then injects effector proteins alter behaviour. Next, enters into targeted cell within an early membrane-bound compartment that matures a slow growing, replicative niche called Containing Vacuole (SCV). Alternatively, pathogen disrupts replicate at high...
ABSTRACT Shigella flexneri , the causative agent of bacillary dysentery, induces massive cytoskeletal rearrangement, resulting in its entry into nonphagocytic epithelial cells. The bacterium-engulfing membrane ruffles are formed by polymerizing actin, a process activated through injected bacterial effectors that target host small GTPases and tyrosine kinases. Once inside cell, S. escapes from endocytic vacuole within minutes to move intra- intercellularly. We quantified fluorescence signals...
Abstract The production of antimicrobial peptides, such as the cathelicidins, plays a prominent role in innate immune response against microbial pathogens. Cathelicidins are widely distributed amongst living organisms, and peptides generated by proteolysis precursor forms typically cationic α‐helical, structure that facilitates their interaction insertion into anionic bacterial cell walls membranes, causing damage promoting death. Here, we found mouse cathelicidin ( Camp ) expression was...
The Gram-negative bacteria of the Burkholderia species are associated with human diseases ranging from pneumonia to life-threatening melioidosis. Upon infection through inhalation, ingestion, or percutaneous route, these can spread and establish granuloma-like lesions resulting fusion host cells form multinucleated giant (MNGCs). resistance several antibiotics highlights importance better understand how innate immune system controls infections.
Abstract Plasma membrane rupture (PMR) and the release of cytosolic content are hallmarks necrotic cell death. The plasma protein ninjurin-1 (NINJ1) actively promotes lysis by polymerizing into embedded filaments that induce formation large lesions. Yet signals controlling NINJ1 polymerization opening lesions remain unknown. Here we combine biology biophysical measurements to characterize steps preceding NINJ1-induced PMR in cells undergoing ferroptosis. Our results show form through a...
Abstract Targeted and specific induction of cell death in individual or groups cells holds the potential for new insights into response tissues organisms to different forms death. Here we report development optogenetically-controlled effectors (optoCDEs), a novel class optogenetic tools that enables light-mediated three types programmed (PCD) – apoptosis, pyroptosis necroptosis using Arabidopsis thaliana photosensitive protein Cryptochrome2. OptoCDEs enable rapid highly PCD human, mouse...
Abstract Inflammasome-induced pyroptosis comprises a key cell-autonomous immune process against intracellular bacteria, namely the generation of dying cell structures. These so-called pore-induced traps (PITs) entrap and weaken microbes. However, importance extracellular pathogens remains unclear. Here, we report that Type-3 secretion system (T3SS)-expressing Pseudomonas aeruginosa ( P. ) escaped PIT immunity by inducing NLRC4 inflammasome-dependent macrophage response in environment. To...