A5067083919- Genetics, Bioinformatics, and Biomedical Research
- Genomics and Phylogenetic Studies
- Advanced Chemical Sensor Technologies
- Bacteriophages and microbial interactions
- Material Properties and Applications
- Spectroscopy and Laser Applications
- Advanced Scientific Research Methods
- Metabolomics and Mass Spectrometry Studies
- Optical and Acousto-Optic Technologies
- Laser Design and Applications
- Chemical Thermodynamics and Molecular Structure
- Machine Learning in Bioinformatics
- Radioactive element chemistry and processing
- Advanced Materials Characterization Techniques
- Wheat and Barley Genetics and Pathology
- Laser Material Processing Techniques
- Radioactive contamination and transfer
- Optical Polarization and Ellipsometry
- Material Science and Thermodynamics
- Advanced Measurement and Metrology Techniques
- DNA and Biological Computing
- Advanced biosensing and bioanalysis techniques
- Quantum optics and atomic interactions
- Nanopore and Nanochannel Transport Studies
- Cell Image Analysis Techniques
Institute for Analytical Instrumentation
2000-2024
Several decades have passed since the development of revolutionary DNA sequencing method by Frederick Sanger and his colleagues. After Human Genome Project, time interval between technologies began to shrink, while volume scientific knowledge continued grow exponentially. Following sequencing, considered as first generation, new generations were consistently introduced into practice. Advances in next generation (NGS) contributed significantly this trend reducing costs generating massive...
The improved model of DNA melting signal is proposed in the article.The based on power polynomials degree 3 (SP).Based model, a technique for processing has been developed, main result which determination temperature Tm.The new allows to decrease analysis time by increasing measurement pitch while simultaneously narrowing range.Adequacy proofs SP are given.The application results developed and known sigmoid function (SF) compared.The possibility using determining thermocycler tubes holder...
The DNA sequencer "Nanofor SPS", developed at the Institute of Analytical Instrumentation Russian Academy Sciences, implements a method for massively parallel sequencing to decrypt sequence nucleic acids. This allows determination nucleotide in or RNA, containing from several hundred hundreds millions bases. Thus, there is opportunity obtain detailed information about genome various biological entities, including humans, animals, and plants. A crucial part this device software, without which...
Determination of the nucleotide sequence DNA or RNA containing from several hundred to hundreds millions monomers units allows obtain detailed information about genome humans, animals and plants. The deciphering nucleic acids’ structure was learned quite a long time ago, but initially decoding methods were low-performing, inefficient expensive. Methods for acid sequences are usually called sequencing methods. Instruments designed implement sequencers.
 Sequencing new generation (SNP),...
Data processing algorithms related to the highly sensitive laser probing of magnetic nanofluids over a wide range concentrations were developed in this study. An approximation polarization magneto-optical responses based on analytical dependences that result from model orientational ordering particles an external field was conducted. The methods for quantitative polarization-optical analysis and proposed techniques used statistical data form basics nanodiagnostics (quantitative...
The method is proposed for express diagnostics of diseases according to the data mass spectrometric analysis exhaled air. An algorithm calculating probability has been developed and tested. results processing patients treated in two oncological clinics are presented. calculation disease air based on attributing spectrum tested patient mass-spectra corresponding control group. Each group formed by collecting an array spectra from at least ten with same disease. Diagnostics performed...
The paper discusses some peculiarities of the design and development scientific equipment, particularly mass spectrometers, which provide implementation high standard technological analytical testing methods.
Counting the occurrence of different k-mers often causes problems genome assembly. Analysis frequency distribution makes it possible to find assembly errors in already formed contigs. Currently, connection with development instrumentation for genetic analysis, there is an urgent need develop methods assessing quality genomic Such techniques will make assess reliability analysis existing and newly developed devices. In this work, based on various software tools, programs were selected...
The development of next-generation sequencing (NGS) technologies has made a significant contribution to the trend reducing costs and obtaining massive data. Institute for Analytical Instrumentation Russian Academy Sciences is developing hardware-software complex deciphering nucleic acid sequences by method mass parallel (Nanofor SPS). Image processing algorithms play an essential role in solving problems genome deciphering. final part this preliminary analysis raw data base-calling process....