A5001804147- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Occupational exposure and asthma
- CRISPR and Genetic Engineering
- Bone Tissue Engineering Materials
- Occupational Health and Safety Research
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- MicroRNA in disease regulation
- Endoplasmic Reticulum Stress and Disease
- Tissue Engineering and Regenerative Medicine
- Viral Infectious Diseases and Gene Expression in Insects
- Air Quality and Health Impacts
- Nerve injury and regeneration
- Biochemical effects in animals
- CAR-T cell therapy research
- Workplace Health and Well-being
- Spaceflight effects on biology
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Graphene and Nanomaterials Applications
- Luminescence and Fluorescent Materials
- Neurogenesis and neuroplasticity mechanisms
- Pancreatic function and diabetes
- Hemophilia Treatment and Research
- Titanium Alloys Microstructure and Properties
Salford Royal NHS Foundation Trust
2021-2025
Stanford University
2024
University of Manchester
2007-2023
Vellore Institute of Technology University
1969-2023
NIHR Manchester Biomedical Research Centre
2023
Manchester Academic Health Science Centre
2017-2022
Institute of Neurosciences Kolkata
2019-2020
Molecular Theranostics (United States)
2018-2019
Christian Medical College & Hospital
2012-2018
Health and Safety Executive
1995-2016
Background. Recent evidence from both monkey and human studies suggests that the reticulospinal tract may contribute to recovery of arm hand function after stroke. In this study, we evaluated a marker output in stroke survivors with varying degrees motor recovery. Methods. We recruited 95 consecutive patients presenting 6 months 12 years their index stroke, 19 heathy control subjects. Subjects were asked respond light flash rapid wrist flexion; at random, was paired either quiet or loud...
We hypothesized that the AAV2 vector is targeted for destruction in cytoplasm by host cellular kinase/ubiquitination/proteasomal machinery and modification of their targets on capsid may improve its transduction efficiency. In vitro analysis with pharmacological inhibitors serine/threonine kinases (protein kinase A, protein C, casein II) showed an increase (20-90%) AAV2-mediated gene expression. The three-dimensional structure was then analyzed to predict sites ubiquitination...
Background Work-related stress (WRS) is the leading cause of occupational ill-health in education sector UK. Headship believed to be a stressful role although there little current research into head teachers. Changes since late 1980s have meant that findings many existing studies are outdated.
This study mainly deals with an effective one-pot solvothermal synthetic pathway for the preparation of uniformly dispersed zirconium oxide nanoparticles on flattened rough surface reduced graphene (ZrO2/rGO NCs) using aqueous leaf extract Andrographis paniculata. After obtaining detailed information and characterization, anticancer activity synthesized ZrO2/rGO nanocrystals (NCs) was evaluated two human cancer cell lines (A549 HCT116) along one normal line (hMSC). The...
The unfolded protein response (UPR) is a stress-induced cyto-protective mechanism elicited towards an influx of large amount proteins in the endoplasmic reticulum (ER). In present study, we evaluated if AAV manipulates UPR pathways during its infection. We first examined role three major axes, namely, endoribonuclease inositol-requiring enzyme-1 (IRE1α), activating transcription factor 6 (ATF6) and PKR-like ER kinase (PERK) infected cells. Total RNA from mock or HeLa cells were used to...
Recombinant adeno-associated virus vectors based on serotype 8 (AAV8) have shown significant promise for liver-directed gene therapy. However, to overcome the vector dose dependent immunotoxicity seen with AAV8 vectors, it is important develop better that provide enhanced expression at significantly low doses. Since known AAV during intracellular trafficking are targeted destruction in cytoplasm by host-cellular kinase/ubiquitination/proteasomal machinery, we modified specific...
In this paper, we have developed a light-activated single component fluorescent organic nanoparticles for synergistic combination therapy (chemo-photodynamic therapy) utilizing the combined benefits of aggregation-induced emission (AIE) and excited-state intramolecular proton transfer (ESIPT) phenomena. The single-component were synthesized by coupling tetraphenylethylene (TPE) moiety with p-hydroxy phenacyl-chlorambucil conjugate. Then, tetraphenylene-p-hydroxyphenacyl-chlorambucil...
Despite significant advancements with recombinant AAV2 or AAV8 vectors for liver directed gene therapy in humans, it is well-recognized that host and vector-related immune challenges need to be overcome long-term transfer. To these limitations, alternate AAV serotypes (1–10) are being rigorously evaluated. AAV5 the most divergent (55% similarity vs. other serotypes) like AAV1 vector known transduce efficiently. also immunologically distinct by virtue of their low seroprevalence minimal cross...
Parkinson’s disease is the second most common neurodegenerative which affects almost 1 percent of population above age 60. It characterized by loss dopaminergic neurons in striatum and substantia nigra, coupled with formation intracellular Lewy bodies degenerating neurons. Recent evidence suggests endoplasmic reticulum stress as a prominent occurrence progression pathogenesis affected human brain. One cellular defense mechanism to combat due excessive protein accumulation through activation...
Although neural stem cells (NSCs) have potential applications in treating neurological disorders, much still needs to be understood about the differentiation biology for their successful clinical translation. In this study, we aimed at deriving NSCs from human umbilical cord blood-derived mesenchymal (hUCB-MSCs) and explored role of Notch signaling process. The hUCB-MSCs were characterized as per guidelines International Society Cellular Therapy. successfully generated by using epidermal...