A5029416116- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Seed Germination and Physiology
- Digestive system and related health
- Reproductive Health and Technologies
- Renal and related cancers
- Cancer Cells and Metastasis
- Magnetic and Electromagnetic Effects
University of Michigan
2022
Pluripotent-stem-cell-derived human intestinal organoids (HIOs) model some aspects of development and disease, but current culture methods do not fully recapitulate the diverse cell types complex organization intestine are reliant on 3D extracellular matrix or hydrogel systems, which limit experimental control translational potential for regenerative medicine. We describe suspension as a simple, low-maintenance method culturing HIOs promoting in vitro differentiation an organized serosal...
The in vitro maturation, fertilization, and morphological changes of goat cumulus–oocyte complexes (COCs) cryopreserved by vitrification using DPBS + 0.5% sucrose 0.4% BSA 4 M, 6 8 or 10 M concentrations glycerol (G) ethylene glycol (EG) were studied. Oocytes holding them over LN vapor for 2-3 min then plunging into LN. After 7–10 days storage, the COCs rewarmed used maturation fertilization 2 experiments to record damage due vitrification, nuclear 28 h after culture (9 replicates), 24...