A5038571614- RNA Interference and Gene Delivery
- Nerve injury and regeneration
- RNA Research and Splicing
- Neurogenesis and neuroplasticity mechanisms
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Bone and Joint Diseases
- Genetics and Neurodevelopmental Disorders
- NF-κB Signaling Pathways
- Spectroscopy Techniques in Biomedical and Chemical Research
- Autism Spectrum Disorder Research
- Signaling Pathways in Disease
- Gold and Silver Nanoparticles Synthesis and Applications
- Osteoarthritis Treatment and Mechanisms
- Spectroscopy and Chemometric Analyses
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- RNA regulation and disease
- Mesenchymal stem cell research
- Virus-based gene therapy research
University of Trieste
2009-2018
Karolinska Institutet
2012-2015
Istituti di Ricovero e Cura a Carattere Scientifico
2015
Institut des Sciences Cognitives Marc Jeannerod
2009
Abstract The predominant mechanism by which adipose mesenchymal stem cells (AMSCs) participate to tissue repair is through a paracrine activity and their communication with the inflammatory microenvironment essential part of this process. This hypothesis has been strengthened recent discovery that release not only soluble factors but also extracellular vesicles, elicit similar biological themselves. We demonstrated treatment cytokines increases immunosuppressive anti-inflammatory potential...
Alternatively spliced brain-derived neurotrophic factor (BDNF) transcripts are targeted to distinct cellular compartments in neurons but the mechanisms underlying this sorting unknown. Although only some BDNF isoforms dendrites, we have found that coding region common all contains a constitutively active dendritic targeting signal and is suppressed containing exons 1 or 4, which restricted cell soma proximal dendrites. This mediated by translin, an RNA-binding protein implicated RNA...
Structural determinants underlying signaling specificity in the tumor necrosis factor receptor superfamily (TNFRSF) are poorly characterized, and it is unclear whether different outputs can be genetically dissociated. The p75 neurotrophin (p75(NTR)), also known as TNFRSF16, a key regulator of trophic injury responses nervous system. Here, we describe genetic approach for dissecting p75(NTR) deciphering its logic. important regulation cell death, NF-κB, RhoA pathways were identified death...
Signaling by the p75 neurotrophin receptor (p75NTR) is often referred to as cell-context dependent, but neuron-type specific signaling p75NTR has not been systematically investigated. Here, we report that signals very differently in hippocampal neurons (HCNs) and cerebellar granule (CGNs), present evidence indicating this partly controlled differential proteolytic cleavage. NGF induced caspase-3 activity cell death HCNs CGNs, while it stimulated NFκB CGNs HCNs. displayed different patterns...
Sorting of mRNAs in neuronal dendrites relies upon inducible transport mechanisms whose molecular bases are poorly understood. We investigated here the mechanism dendritic targeting rat Brain-derived neurotrophic factor (BDNF) as a paradigmatic example. BDNF encodes multiple with either short or long 3'UTR, both hypothesized to harbor signals. However, sorting two 3'UTR isoforms controversial. found that localization was induced by depolarization and NT3 vitro seizures vivo required CPEB-1,...
The neurotrophin brain-derived neurotrophic factor (BDNF) is a key regulator of neuronal development and plasticity. BDNF major pharmaceutical target in neurodevelopmental psychiatric disorders. However, pharmacological modulation this challenging because generated by multiple, alternatively spliced transcripts with different 5′- 3′UTRs. Each mRNA variant transcribed independently, but translation regulation unknown. To evaluate the translatability transcripts, we developed an vitro...
Dendritic trafficking and translation of brain-derived neurotrophic factor (BDNF) transcripts play a key role in mediating synaptic plasticity. Recently, we demonstrated that siRNA-mediated knockdown translin, an RNA-binding protein, impairs KCl-induced dendritic BDNF mRNA cultured hippocampal neurons. We have now assessed whether translin deletion neurons vivo. found its partner trax, undergo translocation response to treatment with pilocarpine, pro-convulsant muscarinic agonist increases...
Raman hyperspectral imaging is an emerging practice in biological and biomedical research for label free analysis of tissues cells.
Introduction The predominant mechanism by which adipose mesenchymal stem cells (AMSCs) participate to tissue repair is through a paracrine activity and their communication with the inflammatory microenvironment essential part of this process. This hypothesis has been strengthened recent discovery that release not only soluble factors but also extracellular vesicles, elicit similar biological themselves. most prominent vesicles are exosomes, delivery transfer protein, bio‐active lipid nucleic...
INTRODUCTION The capacity of adipose mesenchymal stem cells (AMSCs) to secrete a variety trophic factors with diverse functions has motivated the interest evaluating their local or systemic injection stimulate tissue repair in different pathologies, including joint inflammatory diseases. major mechanism by which AMSCs stimulates is paracrine activity and interaction microenvironment seem have critical role. It recently been demonstrated that AMSCs, addiction soluble factors, release also...