A5102750946- Genomics and Phylogenetic Studies
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Probiotics and Fermented Foods
- Renal Diseases and Glomerulopathies
- Bacterial Genetics and Biotechnology
- Advanced biosensing and bioanalysis techniques
- Mycobacterium research and diagnosis
- Photochromic and Fluorescence Chemistry
- Chronic Kidney Disease and Diabetes
- Renal and related cancers
- Virus-based gene therapy research
- Soft tissue tumors and treatment
- Viral gastroenteritis research and epidemiology
- Genetic and Environmental Crop Studies
- Viral Infections and Vectors
- Mitochondrial Function and Pathology
- Biomedical and Engineering Education
- Transgenic Plants and Applications
- Animal Virus Infections Studies
- Neonatal Respiratory Health Research
- RNA and protein synthesis mechanisms
- Lymphatic System and Diseases
- Bioinformatics and Genomic Networks
- Plant Virus Research Studies
Philipps University of Marburg
2021-2025
Loewe Center for Synthetic Microbiology
2022-2024
Friedrich Schiller University Jena
2020-2021
Universitätsmedizin Greifswald
2014-2018
Proteinortho is a widely used tool to predict (co)-orthologous groups of genes for any set species. It finds application in comparative and functional genomics, phylogenomics, evolutionary reconstructions. With rapidly increasing number available genomes, the demand large-scale predictions also growing. In this contribution, we evaluate implement major algorithmic improvements that significantly enhance speed analysis without reducing precision. Graph-based detection (co-)orthologs typically...
Abstract Podocyte loss and changes to the complex morphology are major causes of chronic kidney disease (CKD). As incidence is continuously increasing over last decades without sufficient treatment, it important find predicting biomarkers. Therefore, we measured urinary mRNA levels podocyte genes NPHS1, NPHS2, PODXL BDNF, KIM‐1, CTSL by qRT‐PCR 120 CKD patients. We showed a strong correlation between BDNF injury marker KIM‐1 , which were also correlated with NPHS1 suggesting podocytes as...
Bacterial RNA polymerases (RNAP) utilize 6S RNAs as templates to synthesize ultrashort transcripts (up ~ 14 nt), termed product (pRNAs), that play a key role in reversing the blockage of RNAP by RNA. Here we resolved pRNA length profile 6 S-1 from Bacillus subtilis, major model system for study biology, during outgrowth cells extended stationary phase. 9-mers were found be particularly abundant species, followed 8/10/11-mers and 13/14-mers. Consistent with vitro data Escherichia coli system,...
Background and Purpose Therapeutic options for treating glomerulopathies, the main cause of chronic kidney disease, are limited. Podocyte dedifferentiation is a major event in pathogenesis glomerulopathies. The goal present study was, therefore, to develop an assay monitor podocyte differentiation suitable compound screening. Experimental Approach We isolated cultured glomeruli from transgenic mice, expressing cyan fluorescent protein (CFP) under control promoter nephrin, marker...
Rocaglates are potent broad-spectrum antiviral compounds with a promising safety profile. They inhibit viral protein synthesis for different RNA viruses by clamping the 5′-UTRs of mRNAs onto surface helicase eIF4A. Apart from natural rocaglate silvestrol, synthetic rocaglates like zotatifin or CR-1-31-B have been developed. Here, we compared effects on 5′-UTR-mediated reporter gene expression and binding to an eIF4A-polypurine complex. Furthermore, analyzed cytotoxicity several human immune...
Abstract Leukotrienes are pro-inflammatory lipid mediators generated by 5-lipoxygenase aided the 5-lipoxygenase-activating protein (FLAP). BRP-201, a novel benzimidazole-based FLAP antagonist, inhibits leukotriene biosynthesis in isolated leukocytes. However, like other antagonists, BRP-201 fails to effectively suppress formation blood, which limits its therapeutic value. Here, we describe encapsulation of into poly(lactide- co -glycolide) (PLGA) and ethoxy acetalated dextran (Ace-DEX)...
Elevated glomerular pressure represents a high risk for the development of severe kidney diseases and causes an increase in mechanical load to podocytes. In this study, we investigated whether stress alters gene expression cultured podocytes using arrays. We found that tetraspanin CD9 is significantly upregulated after stress. The differential was confirmed by RT-PCR Western blotting under stretched unstretched conditions. Furthermore, resulted relocalization CD9. To get insight into...
Transcription of non-segmented negative sense (NNS) RNA viruses follows a stop-start mechanism and is thought to be initiated at the genome's very 3'-end. The synthesis short abortive leader transcripts (leaderRNAs) has been linked transcription initiation for some NNS viruses. Here, we identified leaderRNAs (as well as trailer RNAs) that are specifically opposite (anti)genome nt 2; predominantly terminated in region ~ 60-80. LeaderRNA requires hexamer phasing 3'-leader promoter. We...
Three biodegradable block copolymers composed of a poly(ethylene glycol) and copolypeptide with varying compositions cationic l -lysine hydrophobic benzyl- -glutamate were designed for gene delivery applications.
The construction of complex synthetic gene circuits with predetermined and reliable output depends on orthogonal regulatory parts that do not inadvertently interfere the host machinery or other circuit components. Previously, extracytoplasmic function sigma factors (ECFs), a diverse group alternative distinct promoter specificities, were shown to have great potential as context-independent regulators, but so far, they only been used in few model species. Here, we show alphaproteobacterium...
A 60-membered library of vitamin A-functionalized P(MMA-<italic>stat</italic>-DMAEMA)-<italic>b</italic>-PPEGMA block copolymers was synthesized by RAFT polymerization. From these, nanoparticles containing genetic material were formulated and fully characterized.
Drug Prescribing for Patients with Chronic Kidney Disease in General Practice: a Cross-Sectional Study
Galaxy hosts a vast array of tools, tutorials, and workflows, with the exact number workflows remaining uncertain. To address challenge enhancing tool visibility within this expansive ecosystem, pipeline called Tool Metadata Extractor was created during BioHackathon Europe 2023. This aggregates suites from various sources, automatically extracts metadata such as bio.tools identifiers EDAM ontology, presents information in an interactive table. Users can filter table to find tools relevant...
Crop diseases caused by pathogens critically affect global food security and plant ecology. Pathogens are well adapted to their host plants have developed sophisticated mechanisms allowing successful colonization. Plants in turn taken measures counteract pathogen attacks resulting an evolutionary arms race. Recent studies provided mechanistic insights into how two Kiwellin proteins from Zea mays mitigate the activity of chorismate mutase Cmu1, a virulence factor secreted fungal Ustilago...
Abstract Background 6S RNA is a regulator of cellular transcription that tunes the metabolism cells. This small non-coding found in nearly all bacteria and among most abundant transcripts. Lactic acid (LAB) constitute group microorganisms with strong biotechnological relevance, often exploited as starter cultures for industrial products through fermentation. Some strains are used probiotics while others represent potential pathogens. Occasional reports within this already indicate striking...
<p>A 60-membered library of vitamin A-functionalized P(MMA-<i>stat</i>-DMAEMA)-<i>b</i>-PPEGMA block copolymers was synthesized by RAFT polymerization. Subsequently, retinoic acid coupled to hydroxyl groups present in the hydrophilic PPEGMA block. The polymers were investigated for their ability encapsulate ribonucleic acids through nanoparticle (NP) formulation using emulsion/solvent evaporation method. localization A surface-near regions NPs indicated surface...
A 60-membered library of vitamin A-functionalized P(MMA- stat -DMAEMA)- b -PPEGMA block copolymers was synthesized by RAFT polymerization. Subsequently, retinoic acid coupled to hydroxyl groups present in the hydrophilic PPEGMA block. The polymers were investigated for their ability encapsulate ribonucleic acids through nanoparticle (NP) formulation using emulsion/solvent evaporation method. localization surface-near regions NPs indicated surface enhanced Raman spectroscopy, and interaction...
In the present study, three biodegradable block copolymers composed of a poly(ethylene glycole) and copolypeptide with varying compositions cationic L-lysine (L-Lys) hydrophobic benzyl-L-glutamate (Bzl- L-Glu) were designed for gene delivery applications. The polypeptides synthesized by ring opening polymerization (ROP) after orthogonal deprotection Boc-L-Lys side chains, polymer exhibited an amphiphilic character. To bind or encapsulate plasmid DNA (pDNA), various formulations investigated:...