Purpose Dry eye syndrome is a multifactorial chronic disabling disease mainly caused by the functional disruptions in lacrimal gland. The treatment involves palliation like ocular surface lubrication and rehydration. Cell therapy involving replacement of gland promising alternative for providing long-term relief to patients. This study aimed establish functionally competent cultures in–vitro explore presence stem cells native established in-vitro cultures. Methods Fresh human from patients...

Magnetic hyperthermia therapy (MHT) involves heat generation using magnetic nanoparticles (MNPs) in response to an externally applied alternating current field. These MNPs can be specifically targeted the tumor site for homogenous heating. Compared MHT, conventional methods of HT cause heterogeneous heating and thus poor efficacy cancer treatment. MHT has also been shown effectively eliminate highly chemo- radio-resistant stem cells mass. Due their diagnostic capability as well heat-induced...

Retinoblastoma (Rb) is an intraocular tumor that grows rapidly and poses a threat to sight life. Similar other tumors, there increasing speculation the Rb also contains cancer stem-like cells could influence prognosis response therapy. This study was undertaken in attempt identify putative by characterizing different subpopulations of retinoblastoma.Freshly isolated obtained from unfixed eye specimens (n=7) were analyzed for presence CD44, ABCG2, CXCR4, CD133, CD90 using flow cytometry....

Retinoblastoma (Rb), the most common childhood intraocular malignant tumor, is reported to have cancer stem cells (CSCs) similar other tumors. Our previous investigation in primary tumors identified small sized with low CD133 (Prominin-1) and high CD44 (Hyaluronic acid receptor) expression be putative Rb CSCs using flow cytometry (FSClo/SSClo/CD133lo/CD44hi). With this preliminary data, we now utilized a comprehensive approach of vitro characterization Y79 cell line following CSC enrichment...

Abstract Chronic obstructive pulmonary disease (COPD) patients are at higher risk of developing lung cancer and its metastasis, but no suitable biomarker has been reported for differential diagnosis these patients. Levels serum biomarkers (VEGF, IL-8, MMP-9 MMP-2) were analyzed in patients, which compared with healthy donors (HD). VEGF (P < 0.005) 0.05) significantly COPD than HD. Compared to HD, a decrease IL-8 (~8.1 folds; P 0.0001) an increase (~1.6 levels observed the Cancer showed...

Even though bystander effects pertaining to radiation risk assessment has been extensively studied, the molecular players of induced effect (RIBE) in context cancer radiotherapy are poorly known. In this regard, present study is aimed investigate irradiated tumor cells on counterparts mouse fibrosarcoma (WEHI 164 cells) model. Mice co-implanted with WEHI γ-irradiated a lethal dose 15 Gy and unirradiated (bystander) showed inhibited growth, which was measured terms volume Luc+WEHI based...

Cancer stem cells (CSCs) are increasingly being realized to play a significant role in the mechanism of chemo-, radio-resistance, and metastasis cancer. However, studies for spectral markers CSCs using Fourier transform infrared (FT-IR) circular dichroism (CD) spectroscopy limited literature.In present study, obtained from single cell assay human lung adenocarcinoma (A549) were characterized CD44+/CD24-/low phenotype expression, Hoechst 33342 dye efflux assay, expression stemness genes....

Purpose: Retinoblastoma (Rb) is the most common intraocular malignant tumor, which not only unique but also has unraveled many novel aspects of tumor-suppressor genes. Genetic mutations Rb, loss phosphorylation, and other factors resulted in uncontrolled cell division retinal cells resulting tumor progression. In this study, we have analyzed gene expression patterns unilateral tumors (n = 11) comparison to normal-appearing retina 2) from Rb patients who underwent enucleation for advanced Rb....

At the diagnostic stage, metastasis detection is around 75% in lung cancer patients. Major clinical challenge faced by medical oncologists unpredictable development non-metastatic The literature regarding biomarkers/factors prognosticating patients during follow-up very limited. In this pilot study, levels of serum biomarkers (IL-8, VEGF, MMP-2, MMP-9) were measured at diagnosis stage and these observations evaluated for after median 29.2 months. After follow-up, ∼40% developed metastasis....

Microplasts are large extracellular vesicles originating from migratory, invasive, and metastatic cancer cells. Here, to gain insight into the role of microplasts in progression, we performed a proteomic transcriptomic characterization isolated MCF-7 breast cells treated with macrophage-conditioned medium. These were found be viable, highly metabolically active, indicating that derived these not apoptotic bodies. Transcriptomic/proteomic analyses identified 10273 mRNAs 821 proteins...

According to the World Health Organization (2016), chronic obstructive pulmonary disease (COPD) and lung cancer (along with trachea bronchial cancers) are third sixth among 10 top causes of death globally. The association between COPD has been widely established owing their common endogenous exogenous risk factors. Mechanistically, interlinked diseases in many ways such as oxidative stress-associated DNA damage, inflammation, telomere shortening. An increase well correlated smoking, which is...

Many theories were put forward about origin of cancer and immense research was carried out to understand the mechanisms involved in progression disease. The gain scientific knowledge biology technical advancement treatment modalities resulted improvement clinical outcome therapy. However, recurrence metastasis after therapy poses a major concern clinicians. Resistant nature against therapeutic metastatic potential stem cells (CSCs), which even though form fraction tumor mass, result failure...