Abstract We measured concentrations of SARS-CoV-2, influenza A and B virus, respiratory syncytial virus (RSV), mpox human metapneumovirus, norovirus GII, pepper mild mottle nucleic acids in wastewater solids at twelve treatment plants Central California, USA. Measurements were made daily for up to two years, depending on the plant. using digital droplet (reverse-transcription–) polymerase chain reaction (RT-PCR) following best practices making environmental molecular biology measurements....

Wastewater monitoring can provide insights into respiratory disease occurrence in communities that contribute to the wastewater system. Using daily measurements of RNA influenza A (IAV), syncytial virus (RSV), and human metapneumovirus (HMPV), as well SARS-CoV-2 solids from eight publicly owned treatment works Greater San Francisco Bay Area California between July 2022 early 2023, we identify a "tripledemic" when concentrations IAV, RSV, peaked at approximately same time. HMPV was also...

Human norovirus (HuNoV) is a leading cause of disease globally, yet actual incidence unknown. HuNoV infections are not reportable in the United States, and surveillance limited to tracking severe illnesses or outbreaks. Wastewater monitoring for has been done previously results indicate it present wastewater influent concentrations associated with communities contributing wastewater. However, work mostly monthly samples liquid at one few treatment plants (WWTPs).

Influenza A virus (IAV) causes significant morbidity and mortality in the United States has pandemic potential. Identifying IAV epidemic patterns is essential to inform timing of vaccinations nonpharmaceutical interventions. In a prospective, longitudinal study design, we measured RNA wastewater settled solids at 163 treatment plants across 33 states characterize 2022-2023 influenza season state, health human services (HHS) regional, national scales. onset, offset, duration, peak, intensity...

We measured concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants, influenza A B viruses, syncytial virus, human metapneumovirus, enterovirus D68, parainfluenza types 1, 2, 3, 4a, 4b in aggregate, norovirus genotype II, rotavirus, Candida auris, hepatitis adenovirus, mpox H5 pepper mild mottle virus nucleic acids wastewater solids prospectively at 191 treatment plants 40 states across the United States plus Washington DC. Measurements were made two...

Public health policy impact evaluation is challenging to study because randomized controlled experiments are infeasible conduct, and changes often coincide with non-policy events. Quasi-experiments do not use randomization can provide useful knowledge for causal inference. Here we demonstrate how longitudinal wastewater monitoring of viruses at a small geographic scale may be used in quasi-experimental design evaluate the COVID-19 public policies on spread among university population. We...

We used a sensitive, specific PCR assay to detect mpox clade Ib DNA in over 3000 wastewater samples collected prospectively across the United States. Mpox was detected one sample from location with confirmed case; it not locations no cases.

This article reports on an interdisciplinary evaluation of the pilot phase a community-driven civic science project. The project investigates distribution heavy metals in air pollution using moss growing street trees as bio-indicator two industrial-adjacent neighborhoods Seattle, Washington (USA). One goal ongoing is to meaningfully engage local urban youths (eighth twelfth grade) scientific process scientists, and teach them about environmental health, justice, forestry concepts...

Abstract Heavy metal concentrations often vary at small spatial scales not captured by air monitoring networks, with implications for environmental justice in industrial‐adjacent communities. Pollutants measured moss tissues are commonly used as a screening tool to guide use of more expensive resources, like monitors. Such studies, however, rarely address issues or involve the residents and other decision makers expected utilize results. Here, we piloted community science approach, engaging...

Abstract Wastewater monitoring can provide insights into respiratory disease occurrence in communities that contribute to the wastewater system. Using daily measurements of RNA influenza A (IAV), syncytial virus (RSV), and human metapneumovirus (HMPV), as well SARS-CoV-2 solids from eight publicly owned treatment works Greater San Francisco Bay Area California between July 2022 until early May 2023, we identify a “tripledemic” when concentrations IAV, RSV, peaked at approximately same time....

Abstract Background Human norovirus (HuNoV) is a leading cause of disease globally, yet actual incidence unknown. HuNoV infections are not reportable in the United States, and surveillance limited to tracking severe illnesses or outbreaks. Wastewater monitoring for has been done previously results indicate it present wastewater influent concentrations associated with communities contributing wastewater. However, work mostly monthly samples liquid at one few treatment plants (WWTPs)....

Since the start of coronavirus-19 pandemic, use wastewater-based epidemiology (WBE) for disease surveillance has increased throughout world. Because wastewater measurements are affected by external factors, processing WBE data typically includes a normalization step in order to adjust (e.g., viral ribonucleic acid (RNA) concentrations) account variation due dynamic population changes, sewer travel effects, or laboratory methods. Pepper mild mottle virus (PMMoV), plant RNA abundant human...

Since the start of coronavirus-19 pandemic, use wastewater-based epidemiology (WBE) for disease surveillance has increased throughout world. Because wastewater measurements are affected by external factors, processing WBE data typically includes a normalization step in order to adjust (e.g. viral RNA concentrations) account variation due dynamic population changes, sewer travel effects, or laboratory methods. Pepper mild mottle virus (PMMoV), plant abundant human feces and wastewater, been...

Abstract Influenza A virus (IAV) causes significant morbidity and mortality in the United States has pandemic potential. Identifying IAV epidemic patterns is essential to inform timing of vaccines non-pharmaceutical interventions. In a prospective, longitudinal study design, we measured RNA wastewater settled solids at 163 treatment plants across 33 states characterize 2022-2023 influenza season state, health human services (HHS) regional, national scales. onset, offset, duration, peak,...

Abstract We used wastewater monitoring data to evaluate the impact of public health policies and interventions on spread COVID-19 among a university population. first evaluated correlation between incident, reported cases SARS-CoV-2 RNA concentrations observed changes over time. Using difference-in-differences approach, we association policy levels in wastewater. Policy associated with significant change campus included face covering recommendations, indoor gathering bans, routine...

Abstract Heavy metals concentrations often vary at small spatial scales not captured by air monitoring networks, with implications for environmental justice in industrial-adjacent communities. Pollutants measured moss tissues are commonly used as a screening tool to guide use of more expensive resources, like monitors. Such studies, however, rarely address issues or involve the residents and other decision-makers expected utilize results. Here, we piloted community science approach, engaging...

Abstract Purpose : Old-growth forests in the Pacific Northwest host a variety of epiphytes on their branches and stem. Given common often large epiphytic biomass associated with Acer macrophyllum (Pursh ) this region, we evaluated how seasonal weather changes urbanization (metal nitrogen deposition), affect canopy N 2 fixation Hoh Rainforest Olympic Peninsula urban parks Seattle. Methods We collected Isothecium stoloniferum (Brid.) samples from both Seattle at four periods April 2016 through...