Molecular methods based on the 16S rRNA gene sequence are used widely in microbial ecology to reveal diversity of populations environmental samples. Here we show that a new PCR method using an engineered polymerase and 10-nucleotide "miniprimers" expands scope detectable sequences beyond those detected by standard longer primers Taq polymerase. After testing silico identify divergent ribosomal genes previously cloned sequences, applied soil mat samples, which revealed novel would not have...

ABSTRACT The brown rock sea cucumber ( Holothuria glaberrima ) microbiome is vital for understanding its ecological dynamics. This study focuses on the microbial composition and abundance of fungi across different intestinal regions H. glaberrima, giving insights into host-microbe interactions supporting future comparative studies their regeneration capabilities.

The development of techniques that could be useful in fields other than biological warfare agents countermeasures such as medical diagnostics, industrial microbiology, and environmental applications have become a very important subject research. Raman spectroscopy can used near field or at long distances from the sample to obtain fingerprinting information chemical composition microorganisms. In this research, biochemical components cell wall endospores Bacillus thuringiensis ( Bt ) were...

Abstract Mid‐infrared laser spectroscopy was used to investigate common bacteria encountered in biopharmaceutical industries. The study involved the detection of using quantum cascade coupled a grazing angle probe (QCL‐GAP). Substrates similar surfaces commonly industries were as support media for samples. Reflectance measurements assisted by Multivariate Analysis (MVA) assemble powerful spectroscopic technique with classification and identification resources. species analyzed,...

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FTIR, Raman spectroscopy and Surface Enhanced Scattering (SERS) requires a minimum of sample allows fast identification microorganisms. The use this technique for characterizing the spectroscopic signatures these agents their stimulants has recently gained considerable attention due to fact that techniques can be easily adapted standoff detection from distances. also show high sensitivity selectivity offer near real time duty cycles. This research focuses in laying grounds differentiation...

Investigations focusing on devising rapid and accurate methods for developing signatures microorganisms that could be used as biological warfare agents' detection, identification, discrimination have recently increased significantly. Quantum cascade laser (QCL)-based spectroscopic systems revolutionized many areas of defense security including this area research. In contribution, infrared spectroscopy detection based QCL was to obtain the mid-infrared (MIR) spectral Bacillus thuringiensis,...

In Puerto Rico, the microbial diversity of thermal spring (ThS) in Coamo has never been studied using metagenomics. The focus our research was to generate a metagenomic library from ThS Coamo, Rico and explore functional diversity. waters generated direct DNA isolation. High molecular weight (40 kbp) end-repaired, electro eluted ligated into fosmid vector (pCCFOS1); then transduced Escherichia coli EPI300-T1R T1 bacteriophages. consisted approximately 6000 clones, 90% containing DNA....

Studies underestimate the microbial diversity and genotypic traits in snails' microbiome. Caracolus marginella, a land snail native to Caribbean islands, can adapt different environments. Our research focused on generation of metagenomic library from C. marginella gut, further explore functional traits. Thirty specimens were collected four regions Puerto Rico. High molecular weight (40 kb) libraries generated using direct DNA isolation method. was end-repaired ligated into pCCFOS1 fosmid...

In this study, the in vivo function and properties of two cytochrome c maturation proteins, CcmF CcmH from Rhodobacter sphaeroides, were analyzed. Strains lacking or both are unable to grow under anaerobic conditions where c-type cytochromes required, demonstrating their critical role assembly these electron carriers. Consistent with observation, strains deficient when assayed permissive growth conditions. contrast, conditions, only contain several soluble membrane-bound cytochromes, albeit...

We designed a week-long laboratory workshop in metagenomics for cohort of undergraduate student researchers. During this course, students learned and utilized molecular biology microbiology techniques to construct metagenomic library from Puerto Rican soil. Pre-and postworkshop assessments indicated learning gains technical knowledge, skills, confidence research environment. Postworkshop construction additional libraries demonstrated retention by the students.

The anthrax lethal factor (LF) is a zinc dependent metalloproteinase that cleaves the majority of mitogen-activated protein kinase kinases and member NOD-like receptor proteins, inducing cell apoptosis. Despite efforts to fully understand Bacillus anthracis toxin components, gastrointestinal (GI) mechanisms have not been elucidated. Previous studies demonstrated gastric ulceration, substantial bacterial growth rate in Peyer's patches. However, complete molecular pathways disease results...

Bromeliads tank water or phytotelma is an eutrophic microenvironment where microorganisms have evolved to resist sudden changes in pH and nutritional competition. Metagenomics studies been poorly studied bromeliads environmental DNA (eDNA) characterization for its deficient Puerto Rico. Therefore, the data represents microbial communities inhabiting phytotelma. eDNA was extracted using Metagenomic Isolation Kit Water. Next-Generation-Sequencing technology (Illumina MiSeq) used sequencing...

The Delta Cooperative Model (DCM) is a dynamic and innovative teamwork design created to develop fundamentals in research skills. High school students the DCM belong Upward Bound Science Math (UBSM) program at Inter American University, Ponce Campus. After workshops on using scientific method, were organized into groups of three with similar interests. Each student had take role within group as either researcher, data analyst, or editor. Initially, each team developed hypothesis-driven ideas...

Recent investigations have focused on the improvement of rapid and accurate methods to develop spectroscopic markers compounds constituting microorganisms that are considered biological threats. Quantum cascade lasers (QCL) systems revolutionized many areas research development in defense security applications, including his area research. Infrared spectroscopy detection based QCL was employed acquire mid infrared (MIR) spectral signatures Bacillus thuringiensis (Bt), Escherichia coli (Ec)...

The sea cucumber (H. glaberrima) is a species found in the shallow waters near coral reefs and seagrass beds Puerto Rico. To characterize taxonomic variety functional profiles present cucumber, metagenomic genetic material was obtained from their intestinal system. Subsequently, DNA sequencing carried out using Illumina Technology Sequence Analysis performed through NMDC - EDGE server. diversity profile displayed that most predominant domain Bacteria (76.56 %), followed by Viruses (23.24 %)...

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Salmonella Typhimurium, a zoonotic and foodborne pathogen, has experienced surge in antibiotic resistance, contributing to global public health crisis. In response this crisis, the isolation studying of bacteriophages gained significance as an alternative biological control and/or treatment modalities combating these bacterial pathogens. This study reports from wastewater at plant Mayagüez, Puerto Rico. MBBL_B MBBL_S, two Typhimurium bacteriophages, were isolated their host range was tested...

ABSTRACT The microbiota in the paddy soils of Lajas Agricultural Experimental Station at University Puerto Rico (LAES-UPR) plays a crucial role agricultural ecosystems. Despite being an experimental station, these represent natural environments supporting rice cultivation. Microbial diversity was evaluated during pre-harvest and post-harvest periods.