A5009436515- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Biosensors and Analytical Detection
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Nanopore and Nanochannel Transport Studies
- Microfluidic and Capillary Electrophoresis Applications
- Analytical chemistry methods development
- Advanced Biosensing Techniques and Applications
University of Oklahoma
2019-2023
Labeling approaches using isobaric chemical tags (e.g., tagging for relative and absolute quantification, iTRAQ tandem mass tag, TMT) have been widely applied the quantification of peptides proteins in bottom-up MS. However, until recently, successful applications these to top-down proteomics limited because tend precipitate "crash" out solution during TMT labeling complex samples making such difficult. In this study, we report a MS platform confidently identifying quantifying low molecular...
Isobaric chemical tag labeling (e.g., TMT) is a commonly used approach in quantitative proteomics, and quantification enabled through detection of low-mass reporter ions generated after MS2 fragmentation. Recently, we have introduced optimized an intact protein-level TMT platform that demonstrated >90% efficiency complex samples with top-down proteomics. Higher-energy collisional dissociation (HCD) utilized for isobaric tag-labeled peptide fragmentation because it produces accurate ion...
Post-translational modifications (PTMs) play critical roles in biological processes and have significant effects on the structures dynamics of proteins. Top-down proteomics methods were developed for applied to study intact proteins their PTMs human samples. However, large dynamic range complexity samples makes challenging. To address these challenges, we a 2D pH RP/RPLC-MS/MS technique that fuses high-resolution separation protein characterization HeLa cell lysate. Our results provide deep...
The development of novel high-resolution separation techniques is crucial for advancing the complex sample analysis necessary high-throughput top-down proteomics. Recently, our group developed an offline 2D high-pH RPLC/low-pH RPLC method and demonstrated good orthogonality between these two formats. Specifically, ultrahigh-pressure long capillary column has been applied as second dimensional low-pH improvement resolution. To further improve throughput sensitivity approach, we online nano-LC...
Isobaric chemical tag labeling for quantification of intact proteins in complex samples is limited due to the tendency precipitate under conditions and increased sample complexity as a result side products (i.e., incomplete or unintended residues). To reduce precipitation conditions, we developed technique remove large proteoforms that allowed characterization small (<35 kDa) using top-down proteomics. We also systematically optimized protein-level Tandem Mass Tag (TMT) obtain optimal...