A5012914057- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Gene Regulatory Network Analysis
- Mosquito-borne diseases and control
- Bacterial Genetics and Biotechnology
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Structure and Dynamics
- Endoplasmic Reticulum Stress and Disease
- Virus-based gene therapy research
- Electrochemical Analysis and Applications
- Evolutionary Algorithms and Applications
- Organic Electronics and Photovoltaics
- Advanced biosensing and bioanalysis techniques
- Luminescence and Fluorescent Materials
- Machine Learning in Bioinformatics
- Gene expression and cancer classification
- Electrocatalysts for Energy Conversion
- Porphyrin and Phthalocyanine Chemistry
- Genetics, Aging, and Longevity in Model Organisms
- HIV Research and Treatment
- Transgenic Plants and Applications
- Electrochemical sensors and biosensors
University of Washington
2023-2024
Massachusetts Institute of Technology
2018-2023
Amherst College
2016
Stanford University
1965
Vanderbilt University
1965
Dartmouth College
1965
City College of New York
1965
University of Rochester
1965
Yale University
1965
Washington University in St. Louis
1965
Protein denoising diffusion probabilistic models are used for the de novo generation of protein backbones but limited in their ability to guide proteins with sequence-specific attributes and functional properties. To overcome this limitation, we developed ProteinGenerator (PG), a sequence space model based on RoseTTAFold that simultaneously generates sequences structures. Beginning from noised representation, PG structure pairs by iterative denoising, guided desired structural attributes. We...
Abstract Protein denoising diffusion probabilistic models (DDPMs) show great promise in the de novo generation of protein backbones but are limited their inability to guide proteins with sequence specific attributes and functional properties. To overcome this limitation, we develop ProteinGenerator, a space model based on RoseTTAfold that simultaneously generates sequences structures. Beginning from random amino acid sequences, our structure pairs by iterative denoising, guided any desired...
The discovery and optimization of biomolecules that reliably function in metazoan cells is imperative for both the study basic biology treatment disease. We describe development, characterization, proof-of-concept application a platform directed evolution diverse interest (BOIs) directly human cells. relies on custom-designed adenovirus variant lacking multiple genes, including essential DNA polymerase protease features allow us to evolve BOIs encoded by genes as large 7 kb while attaining...
Targeted mutagenesis mediated by nucleotide base deaminase-T7 RNA polymerase fusions has recently emerged as a novel and broadly useful strategy to power genetic diversification in the context of vivo directed evolution campaigns. Here, we expand utility this approach introducing highly active adenosine fusion protein (eMutaT7A→G), resulting higher mutation frequencies enable more rapid evolution. We also assess benefits potential downsides using mutator. go on show Escherichia coli that...
Electrochemical analysis is an important skill to teach in chemistry curricula because it a critical tool current high-impact chemical research. Electrochemistry enables researchers analyze variety of systems extending from molecules materials that encompass research themes ranging clean energy substrate activation biological systems. Specifically, can reveal information about catalytic efficiency, the role catalysts, and nature reduction oxidation (redox) processes. Researchers working area...
A complete photophysical characterization of organic molecules designed for use as molecular materials is critical in the design and construction devices such photovoltaics (OPV). The nature a molecule's excited state will be altered employing same chromophoric units but possessing different architectures. For this reason, we examine reactions two BODIPY-based D-A A-D-A molecules, where D donor acceptor. BODIPY (4,4'-difluoro-4-bora-3a,4a-diaza-s-indacene) moiety serves component connected...
The sequence space accessible to evolving proteins can be enhanced by cellular chaperones that assist biophysically defective clients in navigating complex folding landscapes. It is also possible, at least theory, for proteostasis mechanisms promote strict quality control greatly constrain protein space. Unfortunately, most efforts understand how influence evolution rely on artificial inhibition or genetic knockdown of specific chaperones. few experiments perturb pathways generally modulate...
An abstract is not available for this content so a preview has been provided. As you have access to content, full PDF via the ‘Save PDF’ action button.
An abstract is not available for this content so a preview has been provided. As you have access to content, full PDF via the ‘Save PDF’ action button.
Abstract The sequence space accessible to evolving proteins can be enhanced by cellular chaperones that assist biophysically defective clients in navigating complex folding landscapes. It is also possible, however, for proteostasis mechanisms promote strict quality control greatly constrain protein space. Unfortunately, most efforts understand how influence evolution rely on artificial inhibition or genetic knockdown of specific chaperones. few experiments perturb pathways generally modulate...