Abstract Background As a result of aging, skeletal muscle undergoes atrophy and decrease in function. This age-related weakness is known as “sarcopenia”. Sarcopenia part the frailty observed humans. In order to discover treatments for sarcopenia, it necessary determine appropriate preclinical models genes signaling pathways that change with age these models. Methods results To understand changes gene expression occur aging muscles, we generated multi-time-point signature throughout lifespan...

Introduction Although obesity is one of the established risk factors diabetes mellitus, relationship between and diabetic retinopathy (DR) remains unclear in different studies. This study aimed to investigate association DR with four obesity-related indexes, including body mass index (BMI), waist hip ratio (WHR), height (WHtR) adiposity (BAI) patients diabetes. Research design methods We prospectively enrolled 2305 (2305 eyes) Guangzhou Diabetic Eye Study November 2017 December 2019...

Prior studies suggested that canonical Activin Receptor II (ActRII) and BMP receptor (BMPR) ligands can have opposing, distinct effects on skeletal muscle depending in part differential downstream SMAD activation. It was therefore of interest to test ActRII versus settings differentiation vivo. In human cells, both inhibited myogenic differentiation: a SMAD2/3-dependent manner, via SMAD1/5. Surprisingly, neutralizing ActRIIA/B antibody mitigated the negative classes ligands, indicating some...

Chronic inflammation and a decline in mitochondrial function are hallmarks of aging. Here, we show that the two mechanisms may be linked. We found interleukin-6 (IL6) suppresses settings where PGC1 (both PGC1α PGC1β) expression is low. This suppression mediated by JAK1/STAT1/3 axis, which activates HIF1α through non-canonical involving upregulation HIF1A ERRα transcription, subsequent stabilization protein ERRα. HIF1α, turn, inhibits ERRα, master regulator biogenesis, thus contributing to...

Splenic T cells are pivotal to the immune system, yet their function deteriorates with age. To elucidate specific aspects of cell biology affected by aging, we conducted a comprehensive multi–time point single-cell RNA sequencing study, complemented Assay for Transposase Accessible Chromatin (ATAC) and repertoire (TCR) on splenic from mice across 10 different age groups. This map age-related changes in distribution lineages functional states reveals broad composition, including prominent...

Aims To investigate the relationship between choriocapillaris (CC) blood perfusion and severity of diabetic retinopathy (DR) using swept-source optical coherence tomography angiography (SS-OCTA). Methods Type 2 diabetes mellitus in Guangzhou was recruited. DR graded according to standardised seven-field colour retinal images adhering ETDRS scale. CC quantified by evaluating flow deficit (FD) density, FD number size SS-OCTA. Multivariable linear regressions were used evaluate correlation...

Aims To investigate the relationship between retinal vessel density and renal function in patients with diabetes mellitus (DM) using non-invasive optical coherence tomographic angiography (OCTA). Methods This prospective cross-sectional study recruited ocular-treatment-naïve DM registered community of Guangzhou, China. The superficial capillary plexus macula was obtained by swept-source OCTA imaging. Xiangya equation used to calculate estimated glomerular filtrate rate (eGFR). Participants...

Purpose: To evaluate the role of peripapillary retinal nerve fiber layer (pRNFL) and choroidal thickness (pCT) in development progression diabetic retinopathy (DR). Methods: This is a cohort study based on baseline 2-year follow-up data Guangzhou Diabetic Eye Study. Patients with type 2 diabetes mellitus between ages 30 80 years were recruited from communities Guangzhou. DR was graded by seven-field fundus photography after dilation pupil. pRNFL pCT measured via swept-source optical...

Purpose: The purpose of this study was to evaluate the associations between choroidal thickness (CT) and 2-year incidence referable diabetic retinopathy (RDR). Methods: This a prospective cohort study. Patients with type 2 diabetes in Guangzhou, China, aged 30 80 years underwent comprehensive examinations, including standard 7-field fundus photography. Macular CT measured using commercial swept-source optical coherence tomography (SS-OCT) device (DRI OCT Triton; Topcon, Tokyo, Japan)....

Abstract Background The Chinese government has invested significant resources to build many rural healthcare stations. However, in the face of convenient medical paths and accessible resources, utilization rate health services for older adults areas is surprisingly low. This study explored why health-seeking behavior among China was not active. Methods Data were collected through participatory appraisal (PRA) with 108 participants 12 villages southern China. Daily schedule social resource...

Purpose: To establish normative data for macular thickness in Chinese aged 30 to 80 years using the swept-source optical coherence tomography (SS-OCT) device.Methods: The study included 290 normal eyes, 430 NDR eyes and 150 DR of community residents Guangzhou, China. Mean thicknesses Early Treatment Diabetic Retinopathy Study (ETDRS) subfields, central point (CPT), volume was measured by SS-OCT (Triton DRI OCT, Topcon, Tokyo, Japan) Spectral-Domain OCT (SD-OCT; Heidelberg Engineering,...

Chronic kidney disease (CKD) and diabetic retinopathy (DR) are two serious complications of diabetes. However, the association between retinal neurodegeneration in DR renal function decline is still unclear. Our objective was to evaluate by measure estimated glomerular filtration rate (eGFR), macular ganglion cell-inner plexiform layer (GC-IPL), cell complex (GCC) thickness patients with type 2 diabetes mellitus (T2DM).We analyzed baseline data Guangzhou Diabetic Eye Study. T2DM from...

Purpose: This study aimed to investigate the genetic causal relationships among diet-derived circulating antioxidants, primary open-angle glaucoma (POAG), and glaucoma-related traits using two-sample Mendelian randomization (MR). Methods: Genetic variants associated with antioxidants (retinol, ascorbate, β-carotene, lycopene, α-tocopherol, γ-tocopherol) were assessed as absolute metabolic instrumental variables. POAG data derived from a large, recently published genome-wide association...

To report the 6-year incidence, causes and risk factors for vision loss (visual impairment (VI) blindness), among elderly adults in rural southern China.Population-based, cohort study. Initiated 2014, study recruited participants aged 50 older using random cluster sampling from Yangxi County. All eligible were invited to attend interviews comprehensive eye examinations at follow-up between November 2020 March 2021. The WHO categories of used define incident cases VI (3/60≤VA <6/12),...

To assess the relationship between flow and geometric parameters in optical coherence tomography angiography images risk of incident diabetic retinopathy (DR).This prospective, observational cohort study recruited patients with Type 2 diabetes without DR Guangzhou, China, followed up annually. A commercially available device (DRI OCT Triton; Topcon Inc, Tokyo, Japan) was used to obtain a variety (foveal avascular zone area, vessel density, length density) (fractal dimension blood tortuosity)...

To investigate the impact of high myopia on choriocapillaris perfusion and choroidal thickness (CT) in diabetic patients without retinopathy.Healthy subjects with diabetes mellitus were recruited from communities Guangzhou. They divided into four groups according to presence myopia: healthy control (n = 77), 77). Swept-source optical coherence tomography angiography (SS-OCTA) measured CT. Choriocapillaris was quantified using index (CPI).A total 308 (308 eyes) included study. The average CPI...

To evaluate iris volume before and after pupil dilation using swept-source anterior segment optical coherence tomography (SS-ASOCT) investigate the associated factors of change in patients with type 2 diabetes mellitus (T2DM).A cross-sectional study was conducted Zhongshan Ophthalmic Center among T2DM registered community Guangzhou, China. Anterior chamber (ACV), volume, depth (ACD), angle opening distance at 500 μm (AOD 500) diameter were estimated SS-ASOCT (CASIA; Tomey, Nagoya, Japan)....

The purpose of this study was to identify the rate parafoveal vessel density (VD) changes associated with progression from non-diabetic retinopathy (NDR) early stages DR over a year.This longitudinal cohort enrolled diabetic patients Guangzhou community in China. NDR at baseline were included and underwent comprehensive examinations after 1 year. A commercial OCTA device (Triton Plus, Topcon, Tokyo, Japan) employed quantify VD superficial deep capillary plexuses. rates change time incident...

Aims To investigate longitudinal choroid and ganglion cell–inner plexiform layer (GCIPL) changes in type 2 diabetes mellitus (T2DM) patients healthy populations across years. Methods This prospective cohort study included T2DM controls. were divided into mild non-proliferative diabetic retinopathy (NPDR) or non-DR (NDR) groups. Macular choroidal GCIPL thickness was measured using swept-source optical coherence tomography at baseline follow-up after A linear-mixed effect model compared rates...

To explore the correlation between retinal nerve fiber layer (RNFL) thickness by using optical coherence tomography (OCT) and histological measurements in normal adult rats optic transected rats.The RNFL of 36 was scanned a circle 3.46mm far from disc OCT. The two experimental groups were group (n=20 rats) (n=16 rats). latter included 4 (n=4/group) surviving for 1 day, 3, 5 7 days. Then same retina area also measured NF-200 immunohistochemical staining method. Linear regression used to...

The influence of myopia and ocular biometry parameters on diabetic retinopathy (DR) needs further clarification. We aimed to investigate the association between biometrical DR in Chinese people with diabetes mellitus (DM) without any intervention.This cross-sectional study recruited type 2 DM patients no history treatment Guangzhou, China. were obtained by Lenstar (LS900, Haag-Streit AG, Koeniz, Switzerland), including corneal diameter, central thickness (CCT), curvature (CC), anterior...