In this study, bioinformatic analyses were carried out according to the fully coded CP and MP gene regions of agent, using six novel tomato brown rugose fruit virus (ToBRFV) variants obtained from production greenhouses in Antalya, where infection was first detected Turkey global variants. Molecular evolutionary both showed that all distributed three major clades. Population dynamics studies for have shown there very low nucleotide diversity haplotype diversity. The genes indicated almost no...

Seventy-eight tomato and pepper plants showing symptoms were tested for spotted wilt orthotospovirus (TSWV) using specific primers targeting the full coat protein gene (CP) through RT-PCR. Plant samples collected in Denizli region, Türkiye during September 2021. The PCR screening results revealed that 23 infected with TSWV, indicating an infection rate of 29.48%. products subsequently sequenced bidirectionally, obtained sequences deposited GenBank under accession numbers ON323583-84 pepper,...

Pokeweed antiviral protein (PAP) of Phytolacca americana L. (pokeweed) is a single-chain ribosome-inactivating (RIP) characterized by its ability to depurinate plant ribosomes. Here, we isolated, cloned, and expressed the ribosome inactivating gene, designated as pokeweed type 1 (PAP I), from summer leaves collected Black Sea region (Turkey). Our findings presented here provide direct evidence that exogenous application PAP I causes concentration-dependent inhibition Zucchini yellow mosaic...

Marigold (Tagetes erecta L.) plants, also called Mexican or Aztec marigold, with symptoms of shoot proliferation, dwarfing, and reddening were observed in ornamental gardens Van Province (Turkey). Five two them showing three symptomless sampled at the end September 2014. Genomic DNA isolated from symptomatic nonsymptomatic plant leaves was used to amplify 16S rDNA fragments by nested polymerase chain reaction (PCR). Of 5 marigold samples tested PCR, only yielded expected 1.2-kb fragments....

Cucumber mosaic cucumovirus (CMV) and Watermelon potyvirus (WMV) are plant viruses with positive single-stranded RNA genome that infect cucurbits cause economic losses. Both especially pattern deformities in leaves, cucurbit plants. The leaves of 23 melon 28 watermelon plants showing such symptoms were sampled from different regions Iğdır province. Samples without tested by the Reverse Transcription Polymerase Chain Reaction (RT-PCR) using coat protein gene (CP)-specific primers....

The cucumber plant (Cucumis sativus L) is an important cultivated produced worldwide. Cucumber mosaic virus (Cucumber virus, CMV), one of the common viral diseases, causes economic losses by reducing yield and quality plant. In observations performed in Diyarbakır September 2021, plants showing virus-like symptoms such as mosaic, irregular yellowish spots, deformity on leaves were observed. Symptomatic non-symptomatic samples collected subjected to a polymerase chain reaction (PCR) using...

In 2021, a study was conducted in the Denizli region of Türkiye to investigate phylogenetic relationship and presence alfalfa mosaic alfamovirus (AMV) infecting pepper plants exhibiting viral disease symptoms. A total 57 samples were collected, which twenty-four tested positive for AMV with reverse transcriptase polymerase chain reaction (RT-PCR) assays. Samples from displaying virus symptoms gave bands on agarose gel, while healthy yielded negative results. One randomly selected, cloned...

The prevalence of Potato spindle tuber pospiviroid (PSTVd) infection in randomly selected potato tubers was determined via nonisotopic dot blot hybridization and reverse transcriptase polymerase chain reaction (RT-PCR). In January February 2010, 168 seedling Solanum tuberosum, representing 27 cultivars, were received from different producers Turkey. Digoxigenin-labeled RNA probes PSTVd synthesized the cloned PCR products applied to detect viroids extracts isolated dormant tubers. found 7...

Cucumber mosaic virus (CMV) has a broad plant-host range and wide ecological zone distribution. Virus-like symptoms were observed on tobacco fields of Adiyaman province (Turkey) showing conspicuous mottling, greenish patterns severe malformations leaves. A total forty samples tested positive against CMV by reverse transcription polymerase chain reaction (RT-PCR) using coat protein gene specific primers. Five randomly chosen isolates cloned into pGEM T-Easy vector transformed Escherichia coli...

Two main apple growing provinces (Van and Malatya) of East Anatolia were surveyed for the presence Apple mosaic virus (ApMV). Dot-blot hybridization RT-PCR tests implemented to investigate incidence ApMV, testing a total 481 samples collected from commercial orchards. Digoxigenin-labeled RNA probes ApMV synthesized cloned PCR products applied in dot-blot detect extracts isolated fresh leaf tissues. A validated probe (dot-blot) method was adopted infections orchards Anatolia. In order...

Bo uganin antiviral protein (BAP) gene, one of the ribosome inactivating proteins, isolated from Bougainvillea spectabilis Willd. was cloned, expressed and antifungal activities were investigated. The full-length bouganin gene amplified by reverse transcription-PCR using mRNA as template extracted mature leaves. coding region cloned into prokaryotic expression vector pETDuet-1 after amplification with end to specific primers. recombinant plasmid transformed Escherichia coli cells...

Melon plants exhibiting foliar deformations, mosaic pattern in different concentrations of green, and vein banding were found Bingöl, Turkey, 2019. Specimens gathered screened by RT-PCR using capsid protein (CP) gene-specific primer sets to characterize ascertain the possible viral agents related infected plants. About 657 bp 822 DNA fragments observed agarose gel plants, confirming presence CMV (Cucumber cucumovirus) WMV (Watermelon potyvirus). Two from each recovered randomly inserted...

Phytoplasma-like symptoms of leaf yellowing and calyx malformation were observed in eggplant (Solanum melongena L.), upward leaves fruit pepper (Capsicum annuum aerial tuber formation potato (S. tuberosum L.) during the survey performed late season (August to September) 2015 2016 Van province (Turkey). A total 100 samples tested by nested-PCR using universal primer pairs assess sanitary status solanaceous crops characterise phytoplasma isolates. Among them, seven resulted a 1.25 kb DNA...

Abstract A validated RT-PCR method was used to investigate the presence of Apple scar skin viroid (ASSVd) in commercial apple orchards eastern Anatolia. Among three modified and simplified silica-capture based extraction methods, one for mass ascertain ASSVd. The test initially performed from an ASSVd-infected source then applied total RNA preparations fresh leaf tissues trees collected ASSVd found occur trees. 263 samples, 121 were positive infected showed no apparent disease symptoms on...

The coat protein (CP) genes of the genomic RNA 2 severe Turkish isolates Zucchini yellow mosaic virus (ZYMV) from squash and muskmelon [ZYMV-Adana (Ad) ZYMV-Ahlat (Ah), respectively] were cloned, their complete nucleotide sequences deduced amino acids determined. analysis revealed that both ZYMV-CP contained 837 nucleotides encoded for a CP about 31.2 kDa. Phylogenetic trees based on nucleic acid constructed by neighbor joining unweighted pair group mean arithmetic (UPGMA) methods with 100...

Phytoplasma-type symptoms were noted in tomato (Lycopersicum esculentum L.) Bingöl province of Turkey. The remarkable include witch’s broom, rosetting, purple curled and crispy leaves, small leaves the upper branches excessively elongated calyx. Genomic DNA isolation was performed to identify possible pathogens from 11 plants with without symptom. In Nested-PCR test using universal primer sets, bands approximately 1200 bp size obtained 4 samples. Randomly chosen two primed R16F2n/R16R2...

Phytoplasma-like symptoms were observed in cucumbers (Cucumis sativus L.) Van province of Turkey. The major severe dwarfing, witches' broom, rosetting, little leaf, and sterility plants. Genomic DNA 8 symptomatic non-symptomatic plant leaves was isolated for the detection pathogenic DNA. Of cucumber leaf samples tested by nested polymerase chain reaction (Nested-PCR), four yielded expected 1.25-kb fragments when using universal primer pairs R16mF2/R16mR1 R16F2n/R16R2. Randomly selected two...

Virus-like symptoms were observed in alfalfa plants (Medicago sativa L.) grown Van province of Turkey 2019. The dwarfing, mosaic pattern and yellowing leaves, rosetting, decreasing leaf sizes. Genomic RNA 19 symptomatic non-symptomatic leaves extracted for the detection virus using virus-specific primer pair by RT-PCR. Of specimens, six yielded expected 700 bp DNA band severely specimens. Two them randomly selected inserted into a plasmid vector. Obtained recombinant plasmids sequenced both...

In order to have a more detailed understanding of the various factors influencing reverse transcriptase polymerase chain reaction (RT-PCR), number important parameters such as Mg+2, primer, enzyme concentration and others were optimized for detection Prunus necrotic ringspot virus (PNRSV). Using PNRSV isolate with a pair primers, complementary DNA viral genome template, and an appropriate together with magnesium chloride, following optimal conditions identified: primer concentration...

Nuclear ribosomal DNA (nrDNA) sequence data of the Cuscuta genus, which have been considered as one most popular sequences for phylogenetic inference in plants, studied from a perspective agricultural and non-agricultural lands Turkey. The samples spp. were collected different geographical regions Turkey between years 2013-2015. Some other species, not available locally, taken herbarium some research units. In order to study relations isolations made body tissue samples. Conserved on (rDNA)...

Deformed wing virus (DWV), which belongs to the Iflaviridae family, is a devastating causing severe financial losses in honey bee colonies all over world. In 2020 (November), virus-suspected specimens collected from Erzincan province were screened by molecular assays against DWV infection. 6 of 12 samples, an expected approximately 700 bp DNA bands achieved, indicating probably virus-positive. Two randomly selected amplified band suggesting presence was further cloned into pGEM-T Easy vector...

In this study, it is aimed to asses the association of arbuscular mycorrhizal (AM) fungi within colonised rhizosphere Gramineae family members through a survey by using nested- polymerase chain reaction method in Van province (Turkey). From 24 agro-ecological fields, total 82 samples belonging were tested molecular methods. The presence Glomus intraradices and mosseae was ascertained 10 plants eight different species fungus specific primers. Root colonisation ranged from 6 37% average root AM 22%.

Alfalfa mosaic virus (AMV) is one of the most important viral diseases alfalfa plant among forage crop, causing significant annual economic losses. The agent also potential importance to other cultivars such as tomatoes, potatoes, and peppers in cases. identification phylogenetic relationships AMV were carried out by reverse-transcription polymerase chain reaction (RT-PCR), following bacterial cloning. cDNA samples (12) subjected RT-PCR tests using primer pairs, specific for capsid protein...