A5040979954- Reproductive tract infections research
- Yersinia bacterium, plague, ectoparasites research
- Syphilis Diagnosis and Treatment
- Bacillus and Francisella bacterial research
- Bee Products Chemical Analysis
- Bacterial Infections and Vaccines
- Zoonotic diseases and public health
- Pharmacological Effects of Natural Compounds
- Vector-borne infectious diseases
- Streptococcal Infections and Treatments
- Sex work and related issues
- Female Genital Mutilation/Cutting Issues
- HIV, TB, and STIs Epidemiology
- Virology and Viral Diseases
- Allergic Rhinitis and Sensitization
- Viral Infections and Vectors
- Circadian rhythm and melatonin
- Mosquito-borne diseases and control
- Microbial infections and disease research
- Immunodeficiency and Autoimmune Disorders
- Animal Ecology and Behavior Studies
- Dermatology and Skin Diseases
- Plant-based Medicinal Research
- Ethnobotanical and Medicinal Plants Studies
- Gut microbiota and health
State Research Center for Dermatovenereology and Cosmetology
2022-2025
Ministry of Health of the Russian Federation
2022-2025
Nikon (United States)
2024
Alexion Pharma (Switzerland)
2024
Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2017-2018
Russian Scientific Research Institute Microbe
2015-2018
The established phylogeny of the etiological agent plague, Yersinia pestis, is not perfect, as it does take into account strains from numerous natural foci Commonwealth Independent States (CIS). We have carried out PCR and SNP typing 359 whole genome sequencing 51 these plague determined phylogenetic diversity circulating here. They belong to 0.ANT3, 0.ANT5, 2.ANT3, 4.ANT branches antique biovar, 2.MED0, 2.MED1 medieval biovar 0.PE2, 0.PE4a. 0.PE4h, 0.PE4t branches. Based on studies 178 23...
Fifty six Yersinia pestis strains, isolated over the period of more than 50 years in three high-mountain foci Kyrgyzstan (Tien Shan, Alai, and Talas), have been characterized by means PCR single nucleotide polymorphism (SNP) typing methods. Seven these strains were also whole genome sequencing genome-wide SNP phylogenetic analysis. It was found that forty two belong to 0.ANT2, 0.ANT3 0.ANT5 branches. From these, 0.ANT2 branches earlier detected China only, whereas branch identified for Y....
Background: Gonorrhea is a possible cause of pelvic inflammatory complications, reproductive health disorders and adverse pregnancy outcomes. The problem gonococcal antimicrobial resistance remains actual. Aims: analysis incidence, circumstances case detection, methods laboratory examination for infection used in RF. Materials methods: federal statistical surveillance data 2011-2023. Results: 2011-2019, incidence gonorrhea decreased fivefold, total STI - by 2.5 times. In 2020, 13%, 25%....
Background: The gonococcal pathogen is capable of developing resistance to antimicrobials at a rapid rate. Aims: Updating data on the distribution genetic determinants isolates modern Russian population N. gonorrhoeae number antimicrobial drugs using hydrogel microarray technology. Materials and methods: study included 360 collected in Federal State Research Center Dermatovenereology Cosmetology 2019-2023. Results: In gonococcus, active processes are occurring that result redistribution...
Background: Ultraviolet irradiation of the skin can activate cytoplasmic aryl hydrocarbon receptor AhR, which, in combination with its nuclear translocator ARNT binds to promoter FLG gene encoding filaggrin, a protective barrier protein. This suggests that therapeutic effect phototherapy may be due not only immunosuppressive effect, but also stimulation filaggrin production. Aims: evaluate ultraviolet used for various methods on expression levels FLG, AHR and genes patients atopic...
The emergence of multidrug resistance in N. gonorrhoeae is a serious global problem, and gonorrhea may soon become an incurable disease. aim the study was to characterize population Russia from 2015 2023 predict potential spread most concerning clones. A total 996 isolates were examined during analyzed period. Ceftriaxone azithromycin susceptibility testing performed using agar dilution method. Microarray-based assays sequencing employed identify genotypes genetic markers antimicrobial...
Carried out has been genome-wide sequencing of 20 Yersinia pestis strains from all 11 natural plague foci in Russia and some the neighboring states. Phylogenetic analysis on basis 1918 core SNPs, identified genomes these 16 Y. NCBI GenBank, revealed 5 clusters closely related strains. Taking into account, up-dated subspecific classification agent. New taxonomy combined subspecies: major (ssp. ), Caucasian caucasica ulegeisk ulegeica two novel ones - Central Asian central asiatica ) Angolan...
Background. Neisseria gonorrhoeae can rapidly develop resistance to antimicrobial agents due innate mechanisms for the acquisition of genes. Because rapid formation N. used in gonococcal therapy, risk incurable forms disease is high. The purpose study summarize results RU-GASP over a 16-year period and assess trends antimicrobials regimens antibiotic therapy infection Russia.
 Materials methods. Study Objective. included 5356 isolates received from January 2005 December 2021 State...
Objective of the study is to conduct phylogenetic investigation Yersinia pestis strains (medieval biovar) from plague foci Russia and bordering countries, using SNP-analysis genome-wide sequences these strains. Materials methods. Carried out has been sequencing 14 Y. strains, medieval biovar, 13 natural neighboring states, as well their comparison 9 same contained in NCBI GenBank database. Using software products - Wombac 2.0 Bionumerics 7.1, revealed presence 1875 core SNPs, on basis which...
Q fever is a zoonotic infectious disease characterized by fever, malaise, chills, significant weakness, and muscle pain. In some cases, the can become chronic affect inner membranes of heart, such as valves, leading to endocarditis high risk death. Coxiella burnetii (C. burnetii) primary causative agent in humans. This study aims monitor presence C. ticks collected from small mammals cattle Republic Guinea (RG).Rodents were trapped Kindia region RG during 2019-2020, six regions RG. Total DNA...
Many current gonococcal clinical isolates in Russia show atypical taxonomically significant biochemical activity, which leads to species misidentification. Molecular typing of such cultures according Neisseria gonorrhoeae multiantigen sequence (NG-MAST) and multilocus (MLST) protocols assigned them the G807 NG-MAST GENOGROUP/ST1594 MLST that has been predominant recent years. The goal study was analyze molecular mechanisms atypia N. characterized as members MLST. Sixteen this genogroup were...
Background. The number of syphilis cases in the Russian Federation increased significantly 2022. Control heterogeneity Treponema pallidum subtypes is important to monitor emergence and spread antibiotic-resistant strains Aims. To determine molecular resistance macrolides T. isolates isolated Methods. We analyzed DNA from 49 samples clinical material obtained patients dermatovenerological treatment prevention facilities three federal districts (CFD, SFD, SCFD) 2022 with diagnoses primary...
Syphilis belongs to the category of socially significant sexually transmitted infections, control which is one priorities in global health. The beginning twentieth century was marked by discovery causative agent syphilis and subsequent study both characteristics microorganism itself relationships “human population – treponema pallidum” system. review examines main milestones development domestic syphilidology from present. Despite emergence new highly informative research methods, problems...
Background: Many T.pallidum proteins have been evaluated for their diagnostic value. However, there are difficulties in diagnosing asymptomatic forms of syphilis, when sensitivity serologic methods may vary considerably. Therefore, the search more sensitive and specific antigens serological diagnosis syphilis continues. Purpose study: To evaluate potential recombinant T. pallidum Тр0163 Тр0971 as candidate IgG determination sera patients using enzyme immunoassay. Materials methods:...
Currently, despite the persistently high incidence of gonococcal infection, rapid development Neisseria gonorrhoeae resistance to antimicrobial agents increases risk untreatable forms gonorrhea. Monitoring spread antimicrobial-resistant N. is a major priority for national surveillance programs. Started in 2021-2022 Russian Federation, it showed that trend increased sensitivity penicillins, tetracyclines, fluoroquinolones, and macrolides was replaced by an increase number strains resistant...
Background. The enzyme-linked immunosorbent assay based on recombinant analogues of Treponema pallidum immunogenic proteins enables the diagnosis syphilis by determining immunoglobulin G and M. detection specific M is considered an important criterion in diagnostics at early stages. efficiency depends antigens used to detect antitreponemal antibodies clinical stage infection. No consensus exists which exhibit best characteristics for serodiagnosis syphilis, no test systems can differentiate...
Objective. To perform phylogenetic analysis to assess ongoing changes in population structure of Russian clinical isolates N. gonorrhoeae. Materials and Methods. The object the study was 34 gonorrhoeae obtained from dermatovenerological medical institutions Federation period 2022–2023. cultures were spread on chocolate agar with 1% ISOVitalex growth additive VCAT selective (Becton Dickinson, USA). Mass spectrometric studies performed using MALDI-TOF mass spectrometer (Bruker Daltonics,...
The paper contains the data on complex phenotypic and genotypic characteristics of Yersinia pestis strains isolated in Trans-Baikal steppe plague focus before after succession main carrier which took place 1960s. Based PCR-analysis multilocus sequence typing glpD, melB, napA, rhaA , iclR genes, encoding differentially significant properties such as glycerin, melibiose, rhamnose fermentation, isocitrate lyase production, denitrification activity, developed are proofs affiliation studied to...
Objective of the study is to investigate phenotypic and molecular-genetic features perform whole genome sequencing Y. pestis strains isolated in Vietnam. Materials methods. Studied were genotypic peculiarities 20 plague agent different prefectures Carried out was SNP-analysis strains, sequenced genomes 8 strains. Results conclusions. Based on results studies differential biochemical characteristics all investigated attributed oriental biovar main subspecies agent, which confirmed by presence...
Objective of the study is to assess effectiveness methods for molecular diagnostics and identification Yersinia pestis strains in field material obtained from Sarydzhas high-mountain focus Kyrgyz Republic. Materials methods. Investigated were samples material, isolated 2016 plague focus, using conventional laboratory PCR with hybridization-fluorescent electrophoretic registration results. Results conclusions. It demonstrated that a number cases molecular-genetic method has higher resolution...
Background. Neisseria gonorrhoeae is a facultatively anaerobic microorganism which extremely demanding to the composition of nutrient media and cultivation conditions. In situation increasing shortage cost foreign components for preparation solid media, it important study possibility growing hard-to-cultivate microorganisms on domestically produced media. The aim study. To evaluate growth gonococcus colonies two types – chocolate agar with selective additives prepared using imported reagents...
The species Treponema pallidum includes 4 subspecies. According to the bioinformatic analysis, syphilis pathogen T. subsp. was probably separated from causative agents of yaws, bejel, and pinta more than 800 years ago. Its entry into Europe with its subsequent epidemic at end 15th century remains a matter debate. rapid spread in European countries increase incidence disease were most likely due significant genomic rearrangements, which increased infectivity virulence microorganism, as well...