A5086474530- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Non-Destructive Testing Techniques
- Ultrasonics and Acoustic Wave Propagation
- Gene Regulatory Network Analysis
- Single-cell and spatial transcriptomics
- Welding Techniques and Residual Stresses
- Medical Imaging and Pathology Studies
- Genetic Syndromes and Imprinting
- Advanced Fluorescence Microscopy Techniques
- Neurobiology and Insect Physiology Research
- Cell Image Analysis Techniques
- Epigenetics and DNA Methylation
- Nuclear Structure and Function
- Industrial Vision Systems and Defect Detection
- Near-Field Optical Microscopy
- Image Processing Techniques and Applications
- Gene expression and cancer classification
- Influenza Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Infectious Encephalopathies and Encephalitis
- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- Advancements in Photolithography Techniques
Sichuan University
2023-2025
Zhejiang Center for Disease Control and Prevention
2021
Harvard University
2020
Howard Hughes Medical Institute
2020
Wellcome Sanger Institute
2020
University of Illinois Urbana-Champaign
2012-2016
Little is known about the molecular basis of differences in behavior among individuals. Here we report consistent novelty-seeking behavior, across different contexts, honey bees their tendency to scout for food sources and nest sites, reveal some underpinnings this relative foragers that do not scout. Food scouts showed extensive brain gene expression other foragers, including related catecholamine, glutamate, γ-aminobutyric acid signaling. Octopamine glutamate treatments increased...
Abstract Motivation One major goal of single-cell RNA sequencing (scRNAseq) experiments is to identify novel cell types. With increasingly large scRNAseq datasets, unsupervised clustering methods can now produce detailed catalogues transcriptionally distinct groups cells in a sample. However, the interpretation these clusters challenging for both technical and biological reasons. Popular algorithms are sensitive parameter choices, different solutions with even small changes number principal...
Behavior is among the most dynamic animal phenotypes, modulated by a variety of internal and external stimuli. Behavioral differences are associated with large-scale changes in gene expression, but little known about how these regulated. Here we show transcription factor (TF), ultraspiracle (usp; insect homolog Retinoid X Receptor), working complex transcriptional networks, can regulate behavioral plasticity expression. We first that RNAi knockdown USP honey bee abdominal fat bodies delayed...
Some imprinted genes exhibit parental origin specific expression bias rather than being transcribed exclusively from one copy. The physiological relevance of this remains poorly understood. In an analysis brain-specific allele-biased expression, we identified that Trappc9, a cellular trafficking factor, was expressed predominantly (~70%) the maternally inherited allele. Loss-of-function mutations in human TRAPPC9 cause rare neurodevelopmental syndrome characterized by microcephaly and...
Individual differences in behaviour are often consistent across time and contexts, but it is not clear whether such consistency reflected at the molecular level. We explored this issue by studying scouting honeybees two different behavioural ecological contexts: finding new sources of floral food resources a nest site. Brain gene expression profiles food-source nest-site scouts showed significant overlap, despite large associated with contexts. Class prediction ‘leave-one-out’...
Among forager honey bees, scouts seek new resources and return to the colony, enlisting recruits collect these resources. Differentially expressed genes between behaviors genetic variability in scouting phenotypes have been reported. Whole-genome sequencing of 44 Apis mellifera was undertaken detect variants further understand architecture underlying behavioral differences recruits. The median coverage depth 10.01 10.7 X, respectively. Representation bacterial species among unmapped reads...
Abstract Motivation One major goal of single-cell RNA sequencing (scRNAseq) experiments is to identify novel cell types. With increasingly large scRNAseq datasets, unsupervised clustering methods can now produce detailed catalogues transcriptionally distinct groups cells in a sample. However, the interpretation these clusters challenging for both technical and biological reasons. Popular algorithms are sensitive parameter choices, different solutions with even small changes number principal...
The eddy current testing (ECT) technology based on the Inductance-to-Digital Converter (LDC) has advantages of low power consumption and simple signal conditioning circuitry, which contributes to miniaturisation instrument design. Existing defect identification methods primarily focus conventional Analog-to-Digital (ADC)-based ECT. These rely complex analytical models analyze phase amplitude information voltage signal. However, these approaches do not apply LDC-based paper proposes a method...
Structured illumination of optical sectioning has a wide-filed surface scan compared with point-based or line-based scanning. However, axial based on mechanical work-stage sacrifices the measuring time to improves accuracy. Herein, an electrically tunable lens (ETL) is introduced replace in three-dimensional structured microscopy (3D-SIM). The spatial light modulator (SLM), for amplitude modulation transmitted light, generates sinusoidal grating fringes and high-precision phase shift. ETL...
Background: Studies are needed to provide evidence for the immunization strategy of COVID-19 vaccine simultaneously administered with influenza vaccine. Methods: A randomised, open-label, controlled, phase 4 clinical trial was conducted in Zhejiang Province, east China. Eligible healthy adults aged 18–59 years underwent randomization at a ratio 1:1:2 receive one dose inactivated quadrivalent (IIV4) either first (SI1 subgroup, Day 0) or second (SI2 28) CoronaVac, 14 days after CoronaVac (SE...
This protocol is intended for isolation of nuclei from fresh brain tissue in preparation single-nuclei sequencing library using Chromium Single Cell 3’ Reagent Kits v2 or v3 (10X Genomics). For each step, and reagents were kept on ice at 4C, to reduce unwanted gene expression due procedures maintain nuclear integrity.