A5071609308- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- nanoparticles nucleation surface interactions
- Vibrio bacteria research studies
- Crystallization and Solubility Studies
- Olfactory and Sensory Function Studies
- Gold and Silver Nanoparticles Synthesis and Applications
- Microbial Metabolites in Food Biotechnology
- Biochemical Analysis and Sensing Techniques
- Neurobiology and Insect Physiology Research
- Medical Practices and Rehabilitation
- Spectroscopy and Quantum Chemical Studies
- RNA and protein synthesis mechanisms
- Protein Degradation and Inhibitors
- Nanomaterials for catalytic reactions
- Bacteriophages and microbial interactions
- Force Microscopy Techniques and Applications
- Botanical Research and Chemistry
- Steroid Chemistry and Biochemistry
- Nanocluster Synthesis and Applications
- Microbial Metabolic Engineering and Bioproduction
- Biopolymer Synthesis and Applications
- Analytical Chemistry and Chromatography
- Helminth infection and control
- Linguistics and language evolution
Michigan State University
2019-2021
University of Chicago
2018
Massachusetts Institute of Technology
2016-2017
Freie Universität Berlin
2011-2016
Saarland University
2013-2015
Institute of Macromolecular Chemistry
2014
University of Bonn
1975-1982
University of Wisconsin–Madison
1968-1969
Fritz Haber Institute of the Max Planck Society
1959
Augsburg University
1877
The serine/threonine protein phosphatase 1 (PP1) dephosphorylates hundreds of key biological targets by associating with nearly 200 regulatory proteins to form highly specific holoenzymes. However, how these direct PP1 specificity and the ability predict interacting bind from sequence alone is still missing. nuclear targeting subunit (PNUTS) a that, PP1, plays central role in nucleus, where it regulates chromatin decondensation, RNA processing, phosphorylation state fundamental cell cycle...
Significance Directional degradation is an important feature of energy-dependent intracellular proteolysis in all cells. Proteolytic machines can unravel protein substrates from either terminus depending on the site initial recognition. Here, we use single-molecule optical trapping to probe how bacterial AAA+ proteases ClpXP and ClpAP mechanically degrade a model substrate, titin I27 , N terminus. N-terminal unfolding this substrate often accomplished with one or few power strokes much...
Unprotected ("naked") gold nanoparticles with high monodispersity ([d], 5.5± 0.5 nm) were obtained in a facile and single-step microwave-assisted hydrolytic decomposition of the molecular precursor [NMe4][Au(CF3)2]. Given their chloride-free surface chemistry, as-obtained situ functionalized mono-, di-, trivalent thiolated PEG ligands order to study influence multivalent character on stability colloidal solutions. For this purpose, novel tridentate ligand was synthesized previously reported...
Approaches for the controlled formation of gold nanoparticle dimers are investigated. These based on a locally confined surface modification nanoparticles followed by bridging two particles with an organic linker. A key factor in these approaches is use multivalent ligands. Citrate-stabilized immobilized glass and mono- thiol ligands investigated regarding their ability to inactivate sites facing away from glass. successful functionalization only possible if used this step. The application...
Non-sense-mediated mRNA decay (NMD) is a mechanism of translation-dependent surveillance in eukaryotes: it degrades mRNAs with premature termination codons (PTCs) and contributes to cellular homeostasis by downregulating number physiologically important mRNAs. In the NMD pathway, Upf proteins, set conserved factors which Upf1 central regulator, recruit enzymes promote RNA cleavage. mammals, degradation PTC-containing triggered exon-junction complex (EJC) through binding its constituents Upf2...
In light of the importance nanostructured surfaces for a variety technological applications, quest simple and reliable preparation methods ordered, nanometer ranged structures is ongoing. Herein, versatile method to prepare non-close-packed arrangements nanoparticles on centimeter sized by self-assembly described using monodisperse (118–162 nm Ø), amino-functionalized silica as an exploratory example. It shown that arrangement particles governed interplay between electrostatic repulsion...
5-6 nm gold nanoparticles were prepared by hydrolytic decomposition of [NMe4][Au(CF3)2] and functionalized in situ with mono- multivalent thiolated PEG ligands. Time-dependent changes the monitored aqueous NaCl, NaBr, NaI solutions UV-Vis spectroscopy, TEM, HRTEM. The purely sterically protected particles are stable ≤1 M NaCl NaBr solutions, regardless valence At higher concentrations (≥2 M), monovalent stabilized show minor reaction limited colloidal aggregation. In but not a Ostwald...
Two-component signaling systems (TCSs) function to detect environmental cues and transduce this information into a change in transcription. In its simplest form, TCS-dependent regulation of transcription entails phosphoryl-transfer from sensory histidine kinase cognate DNA-binding receiver protein. However, certain cases, auxiliary proteins may modulate TCSs response secondary cues.
Two-component signaling systems (TCSs) are comprised of a sensory histidine kinase and response regulator protein. In to environmental changes, sensor kinases directly phosphorylate their cognate affect gene expression. Bacteria typically express multiple TCSs that insulated from one another regulate distinct physiological processes. There examples cross-regulation between TCSs, but this phenomenon remains relatively unexplored. We have identified regulatory links the ChvG-ChvI (ChvGI)...
In mice, social behaviors such as mating and aggression are mediated by pheromones related chemosignals. The vomeronasal organ (VNO) detects olfactory information from other individuals sensory neurons tuned to respond specific chemical cues. Receptors expressed implicated in selective detection of these Nearly 400 receptor genes have been identified the mouse VNO, but tuning properties individual receptors remain poorly understood, part due lack a robust heterologous expression system. Here...
GsrN is a conserved small RNA that under transcriptional control of the general stress sigma factor, σT, and functions as posttranscriptional regulator Caulobacter crescentus survival multiple conditions. We have defined features structure determine hyperosmotic stress, we applied transcriptomic proteomic methods to identify regulatory targets The 5' end GsrN, which includes cytosine-rich stem-loop structure, necessary for cell after osmotic upshock. both activates represses gene expression...
Abstract Es wird der Einfluß von Kristallisationsbedingungen auf die temporäre Tribolumineszenz des Arsenoliths untersucht. Die Versuchsergebnisse (zunehmende Tribolumineszenz‐Aktivität mit abnehmender Wachstumsgeschwindigkeit und zunehmender Wachstumstemperatur) lassen sich an Hand eines konzentrationsbedingten Einbaus claudetit‐artigen Bereichen oder OH‐Gruppen im Kristall deuten.