A5101783761- Photoacoustic and Ultrasonic Imaging
- Optical Coherence Tomography Applications
- Advanced Fluorescence Microscopy Techniques
- Thermography and Photoacoustic Techniques
- Optical Imaging and Spectroscopy Techniques
- Ophthalmology and Visual Impairment Studies
- Advanced Chemical Sensor Technologies
- Corneal surgery and disorders
- Target Tracking and Data Fusion in Sensor Networks
- Intraocular Surgery and Lenses
- Thermal Regulation in Medicine
- Actinomycetales infections and treatment
- Educational Reforms and Innovations
- Mycobacterium research and diagnosis
- Visual perception and processing mechanisms
- Adaptive optics and wavefront sensing
- Microbial infections and disease research
- Educational Robotics and Engineering
- Vestibular and auditory disorders
- Refrigeration and Air Conditioning Technologies
- Extracellular vesicles in disease
- Identification and Quantification in Food
- Human Resource and Talent Management
- Higher Education and Teaching Methods
- Advanced Battery Technologies Research
Washington University in St. Louis
2021-2022
University of Virginia
2021
University of British Columbia
2011-2013
Sinopec (China)
2013
Xuzhou Medical College
2010
We present a noninvasive method for characterizing the refractive index (RI) and thickness distribution in biological tissues using combined multiphoton microscopy (MPM) optical coherence tomography (OCT) system. Tissue layers are distinguished by MPM OCT images, RI of each layer determined analyzing co-registered images. The precision this is evaluated on four standard samples which water, air, immersion oil cover glass. Precision within ~1% error compared to reference values obtained....
Enabling simultaneous and high-resolution quantification of the total concentration hemoglobin ( [Formula: see text]), oxygen saturation (sO2), cerebral blood flow (CBF), multi-parametric photoacoustic microscopy (PAM) has emerged as a promising tool for functional metabolic imaging live mouse brain. However, due to limited depth focus imposed by Gaussian-beam excitation, quantitative measurements become inaccurate when object is out focus. To address this problem, we have developed...
Multi-scale multimodal microscopy is a very useful technique by providing multiple imaging contrasts with adjustable field of views and spatial resolutions. Here, we present tri-modal microscope combining multiphoton (MPM), optical coherence (OCM) tomography (OCT) for subsurface visualization biological tissues. The advantages the system are demonstrated on various samples. It enables intrinsic including scattering, two-photon excitation fluorescence (TPEF), second harmonic generation (SHG)....
A multiscale multiphoton microscopy (MPM) and optical coherence tomography (OCT) system has been developed using a sub-10 fs Ti:sapphire laser. The performs cross-sectional OCT imaging over millimeter field-of-view en-face high-resolution MPM with submicrometer resolution from the same sample location. With fish cornea, we have demonstrated of cornea tissue layers OCT, zoom-in cells collagen fibers in each layer MPM. MPM/OCT shows potential rapid coarse scan to search for abnormal regions...
In this paper, we present a minimum entropy fusion approach for multisensor data in non-Gaussian environments. We represent the fused form of weighted sum outputs and use varimax norm as information measure. The optimum weights are obtained by maximizing data. solution only depends on empirical distribution sensor makes no specific assumptions about Numerical simulation results provided to show effectiveness proposed approach.
Multi-parametric photoacoustic microscopy (PAM) has emerged as a promising new technique for high-resolution quantification of hemodynamics and oxygen metabolism in the mouse brain. In this work, we have extended scope multi-parametric PAM to longitudinal, cortex-wide, awake-brain imaging with use long-lifetime (24 weeks), wide-field (5 × 7 mm2), light-weight (2 g), dual-transparency (i.e., light ultrasound) cranial window. Cerebrovascular responses window installation were examined vivo,...
Uniquely capable of simultaneous imaging the hemoglobin concentration, blood oxygenation, and flow speed at microvascular level in vivo, multi-parametric photoacoustic microscopy (PAM) has shown considerable impact biomedicine. However, PAM acquisition requires dense sampling thus a high laser pulse repetition rate (up to MHz), which sets strict limit on applicable energy due safety considerations. A similar limitation is shared by high-speed PAM, also uses lasers with rates. To achieve...
Pathological aggregation of Aβ peptides results in the deposition amyloid brain parenchyma (senile plaques Alzheimer’s disease [AD]) and around cerebral microvessels (cerebral angiopathy [CAA]). Our current understanding amyloid-induced microvascular changes has been limited to structure hemodynamics—leaving oxygen-metabolic aspect unattended. In this Letter, we report a dual-contrast photoacoustic microscopy (PAM) technique, which integrates molecular contrast dichroism PAM physiological...
Improving the imaging speed of multi-parametric photoacoustic microscopy (PAM) is essential to leveraging its impact in biomedicine. However, avoid temporal overlap, A-line rate limited by acoustic biological tissues a few megahertz. Moreover, achieve high-speed PAM oxygen saturation hemoglobin, stimulated Raman scattering effect optical fibers has been widely used generate 558 nm from commercial 532 laser for dual-wavelength excitation. fiber length effective wavelength conversion typically...
Instance-incremental learning (IIL) focuses on continually with data of the same classes. Compared to class-incremental (CIL), IIL is seldom explored because suffers less from catastrophic forgetting (CF). However, besides retaining knowledge, in real-world deployment scenarios where class space always predefined, continual and cost-effective model promotion potential unavailability previous a more essential demand. Therefore, we first define new practical setting as promoting model's...
Refractive index (RI) is the optical property of a medium that describes its ability to bend incident light. The corneal refractive an especially important measurement in and intraocular surgery where precise estimation necessary obtain accurate surgical outcomes. In this study, we calculated RI using combined multiphoton microscopy (MPM) coherence tomography (OCT) system. MPM excites detects nonlinear signals including two photon excitation fluorescence (TPEF) second harmonic generation...
Multimodal MPM/OCT is developed for tissue/cellular imaging with femtosecond laser. Cross-sectional OCT and en face MPM are achieved one platform. Imaging demonstrated on cornea its refractive index thickness characterized.
This paper presents tissue imaging with a multimodal multiphoton microscopy (MPM) and optical coherence tomography (OCT) system. The system can acquire multiple contrasts including two-photon excited fluorescence, second harmonic generation, scattering. also level OCT cellular MPM. MPM/OCT is demonstrated on onion skin cornea samples. provides multi-contrast multi-field-of-view which has potential applications in cancer detection diagnosis.
We report a sparse coding-based two-stage image processing technique for noise reduction and contrast enhancement in photoacoustic microscopy (PAM). have assessed the performance of this denoising using multi-parametric PAM images acquired at different optical fluence levels vivo. Our results show that can significantly improve contrast-to-noise ratio (~7 times) visibility microvasculature low-fluence noisy images, enabling us to reduce by factor 5 while maintaining similar quality....