A5072525161- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- DNA Repair Mechanisms
- PARP inhibition in cancer therapy
- Salmonella and Campylobacter epidemiology
- Radiation Therapy and Dosimetry
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Vibrio bacteria research studies
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
University College London
2018-2021
Universidad de Sevilla
2009-2020
Instituto de Biomedicina de Sevilla
2014-2020
Hospital Universitario Virgen del Rocío
2014-2020
Cancer Research UK
2018
Consejo Superior de Investigaciones Científicas
2009
Universidad Autónoma de Madrid
2009
Centro de Biología Molecular Severo Ochoa
2009
Unidades Centrales Científico-Técnicas
2009
Ribosomes are the highly complex macromolecular assemblies dedicated to synthesis of all cellular proteins from mRNA templates. The main principles underlying making ribosomes conserved across eukaryotic organisms and this process has been studied in most detail yeast Saccharomyces cerevisiae. Yeast composed four ribosomal RNAs (rRNAs) 79 (r-proteins). Most r-proteins need be transported cytoplasm nucleus where they get incorporated into evolving pre-ribosomal particles. Due high abundance...
Mrt4 is a nucleolar component of the ribosome assembly machinery that shares notable similarity and competes for binding to 25S rRNA GAR domain with ribosomal protein P0. Here, we show loss function either P0 or results in deficit 60S subunits, which apparently due impaired processing 27S precursors. Mrt4, shuttles between nucleus cytoplasm, defines medium pre-60S particles. In contrast, absent from but present late/cytoplasmic complexes. The absence notably increased amount nuclear Nop7–TAP...
Ribosomal protein L3 is an evolutionarily conserved that participates in the assembly of early pre-60S particles. We report rpl3[W255C] allele, which affects affinity and function translation elongation factors, impairs cytoplasmic maturation 20S pre-rRNA. This was not seen for other mutations or depletion 60S ribosomal proteins. Surprisingly, pre-40S particles containing pre-rRNA form translation-competent 80S ribosomes, inhibition partially suppresses accumulation. The GTP-dependent...
The post-translational modifier ubiquitin is generated exclusively by proteolytic cleavage of precursor proteins. In Saccharomyces cerevisiae, the linear proteins releases and C-terminally fused ribosomal Rpl40 (Ubi1/2 precursor) Rps31 (Ubi3 precursor), which are part mature 60S 40S subunits respectively. this study, we analysed effects ubi3 mutations that interfere with ubiquitin-Rps31 fusion protein. Strikingly, lethal ubi3+P77 mutation, abolished almost completely, led to a rapid G1 cell...
// Francesca Vena 1, * , Ruochen Jia Arman Esfandiari 1 Juan J. Garcia-Gomez Manuel Rodriguez-Justo 2 Jianguo Ma 3 Sakeena Syed Lindsey Crowley Brian Elenbaas Samantha Goodstal John A. Hartley and Daniel Hochhauser Cancer Research UK Drug-DNA Interactions Group, UCL Institute, Paul O'Gorman Building, University College London, London WC1E 6DD, Department of Pathology, EC1M6BQ, EMD Serono Development Billerica 01821, MA, USA These authors have contributed equally to this work Correspondence...
Evolution has provided eukaryotes with mechanisms that impede immature and/or aberrant ribosomes to engage in translation. These basically either prevent the nucleo-cytoplasmic export of these particles or, once cytoplasm, release associated assembly factors, which interfere binding translation initiation factors ribosomal subunit joining. We have previously shown yeast 40S subunits containing 20S pre-rRNA can In this study, we describe cells harbouring dob1–1 allele, encoding a mutated...
In Saccharomyces cerevisiae, the Mrt4 protein is a component of ribosome assembly machinery that shares notable sequence homology to P0 ribosomal stalk protein. Here, we show these proteins can not bind simultaneously ribosomes and moreover, chimera containing first 137 amino acids last 190 from partially complement absence in conditional null mutant. This associated with isolated this strain when grown under restrictive conditions, although its binding weaker than P0. These contain less P1...
Spb4 is a putative ATP-dependent RNA helicase that required for proper processing of 27SB pre-rRNAs and therefore 60S ribosomal subunit biogenesis.To define the timing association this protein with preribosomal particles, we have studied composition complexes copurify tagged by tandem affinity purification (TAP-tagged Spb4).These contain mainly about 50 ribosome biogenesis proteins, primarily components early pre-60S particles.To lesser extent, some factors 90S particles 35S 27SA also...
In Saccharomyces cerevisiae, ribosome biogenesis requires, in addition to rRNA and ribosomal proteins, a myriad of small nucleolar RNAs (snoRNAs) over two hundred protein trans-acting factors. There are factors predicted participate that have not been so far characterized. Here, we report the functional analysis Nucleolar 6 (Nop6) biogenesis. Our results show Nop6 is needed for optimal 40S subunit Deletion NOP6 leads an appropriate 20% reduction 18S levels therefore subunits. This due mild...
Proteostasis needs to be tightly controlled meet the cellular demand for correctly de novo folded proteins and avoid protein aggregation. While a coupling between translation rate co-translational folding, likely involving an interplay ribosome its associated chaperones, clearly appears exist, underlying mechanisms contribution of ribosomal remain explored. The uL3 contains long internal loop whose tip region is in close proximity peptidyl transferase center. Intriguingly, rpl3[W255C]...
Monopolar spindle-one binder (MOBs) proteins are evolutionarily conserved and contribute to various cellular signalling pathways. Recently, we reported that hMOB2 functions in preventing the accumulation of endogenous DNA damage a subsequent p53/p21-dependent G1/S cell cycle arrest untransformed cells. However, question how protects cells from remained enigmatic. Here, uncover as regulator double-strand break (DSB) repair by homologous recombination (HR). supports phosphorylation RAD51...
The virulence of the human and animal pathogen Salmonella enterica serovar Typhimurium is dependent on two type III secretion systems. These systems translocate proteins called effectors into eukaryotic host cells. SlrP a effector with ubiquitin ligase activity. Here, we used complementary proteomic approaches, two-dimensional gel electrophoresis iTRAQ (isobaric tags for relative absolute quantification) to study consequences presence in epithelial We identified 37 that were differentially...