A5061504357- Soybean genetics and cultivation
- Rice Cultivation and Yield Improvement
- GABA and Rice Research
- Plant responses to water stress
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Arsenic contamination and mitigation
- Plant biochemistry and biosynthesis
- Genetic Mapping and Diversity in Plants and Animals
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Bioenergy crop production and management
- Plant nutrient uptake and metabolism
- Plant Genetic and Mutation Studies
- Biofuel production and bioconversion
- Genetics and Plant Breeding
- Plant-Microbe Interactions and Immunity
- Crop Yield and Soil Fertility
- Heavy Metal Exposure and Toxicity
- Wheat and Barley Genetics and Pathology
- Heavy metals in environment
- Soil Carbon and Nitrogen Dynamics
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Essential Oils and Antimicrobial Activity
Dale Bumpers National Rice Research Center
2017-2024
United States Department of Agriculture
2019-2023
Agricultural Research Service
2019-2023
SUNY Oneonta
2023
Cornell University
2002-2019
South Dakota State University
2011-2018
Brookings Institution
2014
University of Nebraska–Lincoln
2004-2009
Pennsylvania State University
2007-2008
Soybean is an important crop that continually threatened by abiotic stresses, especially drought and heat stress. At molecular levels, reduced yields due to stress can be seen as a result of alterations in metabolic homeostasis vegetative tissues. present incomplete understanding stress-associated metabolism identification associated metabolites remains major gap soybean research. A study with goal profile leaf under control conditions (28/24 °C), [28/24 °C, 10% volumetric water content...
A synthetic gene encoding the tandem affinity purification (TAP) tag has been constructed, and TAP assayed for its effects on expression levels subcellular localization by fusion to green fluorescent protein (GFP) as well steroid-dependent translocation nucleus transcription when fused a hybrid glucocorticoid receptor. nuclear signal (NLS) was detected in calmodulin-binding (CBP) domain removed mutation improve usefulness of tag. Additionally, improvements were made, including inhibition...
Core Ideas Residue returned increased organic carbon and reduced bulk density compared to residue not returned. water retention infiltration cover crop soil volumetric content storage. Cover with no crop. the soybean yield by 14% management crops (CC) can conserve moisture thus has a potential increase yield, but its effectiveness varies significantly region cropping system management. A study was conducted at Brookings, SD, on fine‐silty, mixed, superactive, frigid, Calcic/Pachic Hapludolls...
Drought and heat are among the major abiotic stresses that affect soybean crops worldwide. During current investigation, effect of drought, heat, drought plus was compared in leaves two varieties, Surge Davison, combining 2D-DIGE proteomic data with physiology biochemical analyses. We demonstrated how 25 differentially expressed photosynthesis-related proteins RuBisCO regulation, electron transport, Calvin cycle, carbon fixation during stress. also observed higher abundance stress-induced...
A sharp decline in the availability of arable land and sufficient supply irrigation water along with a continuous steep increase food demands have exerted pressure on farmers to produce more fewer resources. viable solution release this is speed up plant breeding process by employing biotechnology programs. The majority biotechnological applications rely information generated from various -omic technologies. latest outstanding improvements proteomic platforms many other but related advances...
The purpose of this project was to identify metabolites, proteins, genes, and promoters associated with water stress responses in soybean. A number these may serve as new targets for the biotechnological improvement drought soybean (Glycine max).We identified genes that are strongly up or down regulated during rapid following removal from a hydroponics system. 163 metabolites showed significant changes roots 93 leaves. largest change root-specific 160-fold increase coumestan coumestrol...
SUMMARY Plants respond to low temperatures by altering the mRNA abundance of thousands genes contributing numerous physiological and metabolic processes that allow them adapt. At post‐transcriptional level, these cold stress‐responsive transcripts undergo alternative splicing, microRNA‐mediated regulation polyadenylation, amongst others. Recently, m 6 A, 5 C other modifications can affect stability RNA were discovered, thus revealing another layer plays an important role in modulating gene...
Summary Forty‐one rice cDNAs encoding protein kinases were fused to the tandem affinity purification (TAP) tag and expressed in transgenic plants. The TAP‐tagged interacting proteins purified from T1 progeny of plants identified by mass spectrometry. Ninety‐five percent recovered. Fifty‐six found interact with other proteins. A number these interactions consistent known complexes species, validating TAP‐tag method Phosphorylation sites on four that interacted either 14‐3‐3 or cyclins.
Abstract The mitogen‐activated protein kinase (MAPK) cascade is an important signaling module that transduces extracellular stimuli into intracellular responses in eukaryotic organisms. An increasing body of evidence has shown the MAPK‐mediated cellular crucial to plant growth and development, as well biotic abiotic stress responses. To date, a total 17 MAPK genes have been identified from rice genome. Expression profiling, biochemical characterization and/or functional analysis were carried...
Early aerial senescence in switchgrass (Panicum virgatum) can significantly limit biomass yields. WRKY transcription factors that regulate could be used to reprogram and enhance All potential genes present the version 1.0 of genome were identified curated using manual bioinformatic methods. Expression profiles flag leaf RNA-Seq datasets analyzed clustering network analyses tools identify both WRKY-associated gene co-expression networks during development onset. We 240 including members RW5...
Mitogen-activated protein kinase (MAPK) genes in eukaryotes regulate various developmental and physiological processes including those associated with biotic abiotic stresses. Although MAPKs some plant species Arabidopsis have been identified, they are yet to be identified soybean. Major objectives of this study were identify GmMAPKs, assess their evolutionary relationships, analyze functional divergence. We a total 38 MAPKs, eleven MAPKKs, 150 MAPKKKs Within the GmMAPK family, we also new...
Abstract Rice ( Oryza sativa L.) is a critical crop for global food security; however, under certain conditions, rice grains can accumulate elevated levels of arsenic (As) and cadmium (Cd). Research has shown that alternate wetting drying (AWD) fields, rather than maintaining continuous flood (FLD), lower concentrations both total As (tAs) the more toxic inorganic (iAs) in milled rice. Unfortunately, irrigation management oxidizes soil potentially result higher Cd concentrations. This 2‐year...
Access to adequate irrigation resources is critical for sustained agricultural production, and rice, a staple cereal grain half of the world population, one biggest users irrigation. To reduce water use, several saving systems have been developed rice but reliable system evaluate cultivars stress tolerance still lacking. Here, seven that diverse yield potential under were evaluated in field study using four continuous regimes varying from saturation wilting point. understand relationship...
Eighty-eight rice (Oryza sativa) cDNAs encoding leaf expressed protein kinases (PKs) were fused to a Tandem Affinity Purification tag (TAP-tag) and in transgenic plants. The TAP-tagged PKs interacting proteins purified from the T1 progeny of plants identified by tandem mass spectrometry. Forty-five recovered this study thirteen these found interact with other high probability score. In vivo phosphorylated sites for three PKs. A comparison data combined analysis 129 concurrent screen using...
Untimely rains in wheat fields during harvest season can cause pre-harvest sprouting (PHS), which deteriorates the yield and quality of crop. Metabolic homeostasis embryo plays a role seed dormancy, determining status maturing grains either as dormant (PHS-tolerant) or non-dormant (PHS-susceptible). Very little is known for direct measurements global metabolites embryonic tissues seeds. In this study, physiologically matured freshly harvested seeds PHS-tolerant (cv. Sukang, dormant)...
Salt stress is a major constraint to rice acreage and production worldwide. The purpose of this study was evaluate the natural genetic variation available in United States Department Agriculture (USDA) mini-core collection (URMC) for early vigor traits under salt identify quantitative trait loci (QTLs) seedling-stage tolerance via genome-wide association (GWAS). Using hydroponic system, seedlings 162 accessions were subjected electrical conductivity (EC) 6.0 dS m-1 at three-to-four leaf...
Sustainable food production is a major challenge to our current agricultural systems. At the global level, water stress one of most critical abiotic factors that affect crop productivity. Currently, vast amount research efforts are being laid down generate molecular information leading an understanding this factor. Although gene expression analysis at transcription level has enhanced regarding response plants drought stress, many questions remain unanswered protein level. In view fact plant...
Decreasing carbon (C) footprints by reducing nitrogen (N) and water inputs has been speculated to have negative impacts on wheat grain yield flour processing quality. The objective of this study was determine the impact N stress winter yield, protein composition, dough Wheat fertilized at two rates (unfertilized recommended) grown under water-stressed well-watered environments. Nitrogen were measured using 13C isotopic approach. Research showed that (1) fertilizer water-management...