A5103459063- Renal Transplantation Outcomes and Treatments
- T-cell and B-cell Immunology
- Organ Transplantation Techniques and Outcomes
- Thin-Film Transistor Technologies
- Transplantation: Methods and Outcomes
- Silicon Nanostructures and Photoluminescence
- Complement system in diseases
- Bariatric Surgery and Outcomes
- Organ Donation and Transplantation
- Silicon and Solar Cell Technologies
- Renal Diseases and Glomerulopathies
- Pluripotent Stem Cells Research
- Methane Hydrates and Related Phenomena
- Spacecraft and Cryogenic Technologies
- Urological Disorders and Treatments
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Cytomegalovirus and herpesvirus research
- Biomedical Ethics and Regulation
- Obesity and Health Practices
- Xenotransplantation and immune response
- Reformation and Early Modern Christianity
- Medieval Literature and History
- Hematopoietic Stem Cell Transplantation
- Esophageal and GI Pathology
Dudley Group NHS Foundation Trust
2015-2024
University of York
2024
Concord Repatriation General Hospital
2010-2024
Sydney Local Health District
2022
Russells Hall Hospital
2020-2021
Augusta University
2021
Cambridge University Hospitals NHS Foundation Trust
2008-2018
National Health Service
2018
NIHR Cambridge Biomedical Research Centre
2018
National Institute for Health Research
2018
The introduction of solid-phase immunoassay (SPI) technology for the detection and characterization human leukocyte antigen (HLA) antibodies in transplantation while providing greater sensitivity than was obtainable by complement-dependent lymphocytotoxicity (CDC) assays has resulted a new paradigm with respect to interpretation donor-specific (DSA). Although SPI assay performed on Luminex instrument (hereafter referred as assay), particular, permitted not detectable CDC, clinical...
The presence of preexisting (memory) or de novo donor-specific HLA antibodies (DSAs) is a known barrier to successful long-term organ transplantation. Yet, despite the fact that laboratory tools and our understanding histocompatibility have advanced significantly in recent years, criteria define DSA assign level risk for given vary markedly between centers. A collaborative effort American Society Histocompatibility Immunogenetics Transplantation provided logistical support generating...
The hydrogen storage capacity of binary THF−H2 clathrate hydrate has been determined as a function formation pressure, THF composition, and time. amount stored in the stoichiometric increases with pressure exhibits asymptotic (Langmuir) behavior to approximately 1.0 wt % H2. This concentration corresponds one molecule occupying each small 512 cavities large 51264 cavity framework. Contrary previous reports, was not increased upon decreasing below 5.6 mol solution 0.5 %, at constant even...
Alemtuzumab is a powerful lymphocyte depleting antibody currently being evaluated in solid organ transplantation. This paper describes 5-year results of single center study alemtuzumab as induction renal Thirty-three transplant recipients received 20 mg on day 0 and 1, followed by half-dose cyclosporin monotherapy (trough concentration 75-125 ng/mL) from 3. They were compared retrospective contemporaneous-controlled manner with 66 kidney transplanted the same period who conventional...
Abstract Objective Variation in the major histocompatibility complex (MHC) on chromosome 6p21 is known to influence susceptibility multiple sclerosis with strongest effect originating from HLA ‐ DRB1 gene class II region. The possibility that other genes MHC independently has been suggested but remains unconfirmed. Methods Using a combination of microsatellite, single nucleotide polymorphism, and human leukocyte antigen (HLA) typing, we screened trio families looking for evidence residual...
Leukocyte Ig-like receptors (LILRs) are a family of innate immune predominantly expressed by myeloid cells that can alter the Ag presentation properties macrophages and dendritic cells. Several LILRs bind HLA class I. Altered LILR recognition due to allelic variation could be contributing factor in disease. We comprehensively assessed binding >90 I alleles. The inhibitory LILRB1 LILRB2 varied their level different alleles, correlating some cases with specific amino acid motifs. displayed...
Antibody-mediated rejection is a major cause of premature graft loss in kidney transplantation. Multiple scoring systems are available to assess the HLA mismatch between donors and recipients at molecular level; however, their correlation with development de novo donor-specific antibody (dnDSA) has not been compared on active immunosuppression.HLA-DRβ1/3/4/5/DQα1β1 was determined using eplet analysis, amino acid mismatch, electrostatic for 596 renal transplant correlated HLA-DR/DQ dnDSA...
In a series of 123 renal transplants performed in the presence positive crossmatch (either peak positive-current or positive—current negative), we have analyzed immunoglobulin class and specificity donor-reactive antibodies. The was determined by dithiothreitol reduction cytotoxicity inhibition using mono-morphic antibodies specific for HLA I, DR, DQ antigens. There good primary graft regraft survival current crossmatches due to IgM non-HLA also acceptable with negative cross-matches T B...
Knowledge of the human leukocyte antigen (HLA) amino acid (AA) sequence combined with crystallographic structural data may enable prediction relative immunogenicity individual donor/recipient HLA mismatches.Multiple sera from 32 highly sensitized patients awaiting kidney transplantation were screened using Luminex/single-antigen beads to determine HLA-specific antibody levels against mismatched class I specificities. A computer program was developed allow intralocus and interlocus comparison...
Background. We have shown previously that human leukocyte antigen (HLA) class I immunogenicity can be predicted by the number, position, and physiochemical differences of polymorphic amino acids (AAs). now modeled structural polymorphisms HLA II alloantigens correlated these with humoral alloimmunity in sensitized patients awaiting kidney transplantation. Methods. Sera obtained from 30 high levels IgG HLA-specific antibodies were screened using single-antigen antibody detection beads. A...
Natural progression of HIV-1 infection depends on genetic variation in the human major histocompatibility complex (MHC) class I locus, and CD8+ T cell response is thought to be a primary mechanism this effect. However, polymorphism within MHC may also alter innate immune activity against immunodeficiency virus type 1 (HIV-1) by changing interactions leukocyte antigen (HLA) molecules with immunoglobulin-like receptors (LILR), group immunoregulatory mainly expressed myelomonocytic cells...
Background: Traditionally the management of acute diverticulitis complicated by perforation has been Hartmann’s procedure, which may be associated with significant morbidity and mortality unpleasantness a colostomy. We present our early experience in managing perforated acutely laparoscopic lavage drainage. Methods: A retrospective review was conducted all patients surgically confirmed diverticulitis. Details concerning nature presentation, operative findings, postoperative course...
The diagnosis of graft-versus-host disease (GvHD) after liver transplantation can be difficult because early symptoms are often nonspecific. In this study, the presence donor lymphocyte macrochimerism in recipient peripheral blood was examined as a diagnostic aid for GvHD cadaveric transplantation.Between 1996 and 2002, 33 transplant recipients with clinical suspicion (skin rash, diarrhea, pyrexia, pancytopenia, or anemia, without an obvious alternative cause) were investigated...