A5088782116- Microbial Natural Products and Biosynthesis
- Enzyme Catalysis and Immobilization
- Plant biochemistry and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Antibiotic Resistance in Bacteria
- Chemical synthesis and alkaloids
- Bioactive Compounds and Antitumor Agents
- Synthetic Organic Chemistry Methods
- Genomics and Phylogenetic Studies
- Algal biology and biofuel production
- Microbial Metabolism and Applications
- Oxidative Organic Chemistry Reactions
- Marine Sponges and Natural Products
- Carbohydrate Chemistry and Synthesis
- Metabolomics and Mass Spectrometry Studies
University of Bristol
2019-2024
A molecular description of the complete reaction cycle bona fide natural Diels–Alderase AbyU is presented, revealing mechanistic intricacies this enzyme system.
Stereoselective carbon-carbon bond forming reactions are quintessential transformations in organic synthesis. One example is the Diels-Alder reaction, a [4+2] cycloaddition between conjugated diene and dienophile to form cyclohexenes. The development of biocatalysts for this reaction paramount unlocking sustainable routes plethora important molecules. To obtain comprehensive understanding naturally evolved cyclases, identify hitherto uncharacterised we constructed library comprising...
Mupirocin is a clinically important antibiotic produced by Pseudomonas fluorescens NCIMB 10586 that assembled complex trans-AT polyketide synthase. The fragment, monic acid, esterified 9-hydroxynonanoic acid (9HN) side chain which essential for biological activity. ester assembly initialised from 3-hydroxypropionate (3HP) starter unit attached to the acyl carrier protein (ACP) MacpD, but fate of this species unknown. Herein we report application NMR spectroscopy, mass spectrometry, chemical...
The abyssomicins are a structurally intriguing family of bioactive natural products that include compounds with potent antibacterial, antitumour and antiviral activities. biosynthesis the characteristic abyssomicin spirotetronate core occurs
Abstract Carbon‐carbon bond formation is a fundamental transformation in both synthetic chemistry and biosynthesis. Enzymes catalyze such reactions with exquisite selectivity which often cannot be achieved using non‐biological methods but may suffer from an intolerance of high temperature the presence organic solvents limiting their applications. Here we report thermodynamic kinetic stability β ‐barrel natural Diels‐Alderase AbyU, catalyzes spirotetronate core antimicrobial product...
The Diels-Alder reaction is one of the most effective methods for synthesis substituted cyclohexenes. development protein catalysts this remains a major priority, affording new sustainable routes to high value target molecules. Whilst small number natural enzymes have been shown capable catalysing [4+2] cycloadditions, there need significant mechanistic understanding how these prospective Diels-Alderases promote catalysis underpin their as biocatalysts use in synthesis. Here we present...
Mupirocin is a clinically important antibiotic produced by