A5091349343- Amyloidosis: Diagnosis, Treatment, Outcomes
- Alzheimer's disease research and treatments
- Protein Interaction Studies and Fluorescence Analysis
- Protein Structure and Dynamics
- Cellular transport and secretion
- Enzyme Structure and Function
- Protein Kinase Regulation and GTPase Signaling
- Protein purification and stability
- Supramolecular Self-Assembly in Materials
- Analytical Chemistry and Chromatography
- Bioactive Compounds and Antitumor Agents
- Synthesis and bioactivity of alkaloids
- Metal complexes synthesis and properties
- HIV/AIDS drug development and treatment
- Phase Equilibria and Thermodynamics
- Drug Transport and Resistance Mechanisms
- HIV-related health complications and treatments
- Free Radicals and Antioxidants
- Food Chemistry and Fat Analysis
- Blood properties and coagulation
- Cholinesterase and Neurodegenerative Diseases
- Graph theory and applications
- Nanoparticle-Based Drug Delivery
- Dye analysis and toxicity
- Endoplasmic Reticulum Stress and Disease
University of Coimbra
2015-2024
Food processing and storage cause natural food color to diminish, leading the addition of synthetic dyes by industry. Unfortunately, these have potential health risks, such as accumulation in blood plasma interaction with human serum albumin (HSA). To address this issue, present study utilized spectroscopic techniques silico calculations report biophysical profile HSA:Amaranth HSA:New coccine interactions. The showed that HSA:Dye is spontaneous (ΔG° < 0) driven enthalpy (ΔH° ≈ −12 kJ mol–1)...
Tenofovir (TFV) is the active form of prodrugs tenofovir disoproxil fumarate (TDF) and alafenamide (TAF), both clinically prescribed as HIV reverse transcriptase inhibitors. The biophysical interactions between these compounds human serum albumin (HSA), primary carrier exogenous in bloodstream, have not yet been thoroughly characterized. Thus, present study reports interaction profile HSA TFV, TDF, TAF via UV–Vis, steady-state, time-resolved fluorescence techniques combined with isothermal...
Ketoprofen (KTF) and ketorolac (KTL) are among the most primarily used non-steroidal anti-inflammatory drugs (NSAIDs) in humans to alleviate moderate pain treat inflammation. Their binding affinity with albumin (the main globular protein responsible for biodistribution of bloodstream) was previously determined by spectroscopy without considering some conventional pitfalls. Thus, present work updates biophysical characterization interactions HSA:KTF HSA:KTL 1H saturation-transfer difference...
The kinetics of transthyretin aggregation from acid-unfolded monomers to amyloid fibrils was studied by several biophysical techniques and may be described as a two-step process with transient accumulation oligomeric species.
The use of enzymes in laundry and dish detergent products is growing. Such tendency implies dedicated studies to understand surfactant‐enzyme interactions. interactions between surfactants their impact on the catalytic efficiency represent a central problem were here evaluated using circular dichroism, dynamic light scattering, enzyme activity determinations. This work focuses this key issue by evaluating role ethyleneoxide (EO) groups anionic structure commercial lipase, focusing...
Protein aggregation into insoluble amyloid fibrils is the hallmark of several neurodegenerative diseases, chief among them Alzheimer’s and Parkinson’s. Although caused by different proteins, these pathologies share some basic molecular mechanisms with familial amyloidotic polyneuropathy (FAP), a rare hereditary neuropathy formation deposition transthyretin (TTR) in peripheral autonomic nervous systems. Among amyloidogenic TTR mutations known, V30M-TTR most common FAP. amyloidogenesis (ATTR)...
One of the molecular hallmarks amyloidoses is ordered protein aggregation involving initial formation soluble oligomers that eventually grow into insoluble fibrils. The identification and characterization species critical for amyloid fibril disease development have been focus intense analysis in literature. Here, using photo-induced cross-linking unmodified proteins (PICUP), we studied early stages oligomerization human transthyretin (TTR), a plasma involved diseases (ATTR amyloidosis) with...
Several degenerative amyloid diseases, with no fully effective treatment, affect millions of people worldwide. These pathologies—amyloidoses—are known to be associated the formation ordered protein aggregates and highly stable insoluble fibrils, which are deposited in multiple tissues organs. The disruption preformed fibrils is one possible therapeutic strategy against amyloidosis; however, only a few compounds have been identified as fibril disruptors vivo date. To properly identify...
In-depth characterization of fundamental folding steps small model peptides is crucial for a better understanding the mechanisms more complex biomacromolecules. We have previously reported on folding/unfolding kinetics α-helix. Here, we study transitions in chignolin (GYDPETGTWG), short β-hairpin peptide used as to conformational changes β-sheet proteins. Although suggested, until now, role Tyr2–Trp9 interaction mechanism was not clear. In present work, pH-dependent were characterized by...
"Lead optimization of resilient next-generation transthyretin stabilizers for multiple target-product profiles: approaching the CNS." Amyloid, 26(sup1), pp. 77–78