A5028622476- DNA Repair Mechanisms
- Fungal and yeast genetics research
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Carcinogens and Genotoxicity Assessment
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- Cancer therapeutics and mechanisms
- Chromosomal and Genetic Variations
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- PARP inhibition in cancer therapy
- Plant Disease Resistance and Genetics
- Genetic Neurodegenerative Diseases
- Endoplasmic Reticulum Stress and Disease
- RNA Research and Splicing
- Genetics, Aging, and Longevity in Model Organisms
- Mitochondrial Function and Pathology
- Genetic factors in colorectal cancer
- Telomeres, Telomerase, and Senescence
- Animal Genetics and Reproduction
- Plant Genetic and Mutation Studies
- Bioinformatics and Genomic Networks
Columbia University Irving Medical Center
2015-2025
Columbia University
2005-2021
Baylor College of Medicine
2019
The University of Texas Health Science Center at Houston
2019
New York University
2019
University of Copenhagen
2019
University of Iowa
2019
Oak Ridge National Laboratory
2004
University of Tennessee at Knoxville
2004
Cold Spring Harbor Laboratory
2002
DNA molecules that integrate into yeast chromosomes during transformation do so by homologous recombination. We have studied the way in which circular and linear recombine with chromosomal sequences. show ends are highly recombinogenic interact directly Circular hybrid plasmids can a single reciprocal crossover, but only at low frequency. Restriction enzyme digestion within region to greatly enhances efficiency of integration. Furthermore, if two restriction cuts made same sequence, thereby...
In Saccharomyces cerevisiae, MEC1 and RAD53 are essential for cell growth checkpoint function. Their role in can be bypassed by deletion of a novel gene, SML1, which functions after several genes whose overexpression also suppresses mec1 inviability. addition, sml1 affects various cellular processes analogous to overproducing the large subunit ribonucleotide reductase, RNR1. These include effects on mitochondrial biogenesis, DNA damage response, growth. Consistent with these observations,...
We have previously shown that cells mutant for TOP3, a gene encoding prokaryotic-like type I topoisomerase in Saccharomyces cerevisiae, display pleiotropic phenotype including slow growth and genome instability. identified mutation, sgs1 (slow suppressor), suppresses both the defect increased genomic instability of top3 mutants. Here we report independent isolation SGS1 screen proteins interact with Top3. DNA sequence analysis reveals putative Sgs1 protein is highly homologous to helicase...
The Saccharomyces cerevisiae RAD52 gene plays a pivotal role in genetic recombination. Here we demonstrate that yeast Rad52 is DNA binding protein. To show the interaction between and direct not mediated by other proteins to facilitate protein purification, recombinant expression system was developed. can bind both single- double-stranded addition of either Mg2+ or ATP does enhance single-stranded DNA. Furthermore, domain found evolutionary conserved N terminus More importantly, stimulates...
Maintenance of genomic integrity and stable transmission genetic information depend on a number DNA repair processes. Failure to faithfully perform these processes can result in alterations subsequent development cancer other diseases. In the eukaryote Saccharomyces cerevisiae , homologous recombination is major pathway for repairing double-strand breaks. The key role played by Rad52 this has been attributed its ability seek out mediate annealing strands. study, we find that S. fused green...
Cell cycle checkpoints are evolutionarily conserved surveillance systems that protect genomic stability and prevent oncogenesis in mammals. One important target of checkpoint control is ribonucleotide reductase (RNR), which catalyzes the rate-limiting step dNTP DNA synthesis. In both yeast humans, RNR transcriptionally induced after damage via Mec1/Rad53 (yeast) ATM/CHK2 (human) pathways. addition, proteins Mec1 Rad53 also regulate inhibitor Sml1. After or at S phase, phosphorylation...
Unesterified sterol modulates the function of eukaryotic membranes. In human cells, is esterified to a storage form by acyl-coenzyme A (CoA): cholesterol acyl transferase (ACAT). Here, two genes are identified, ARE1 and ARE2 , that encode ACAT-related enzymes in yeast. The yeast 49 percent identical each other exhibit 23 identity ACAT. Deletion reduced ester levels approximately 25 normal levels, whereas disruption did not affect biosynthesis. both resulted viable cell with undetectable...