A5047807923- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Cellular transport and secretion
- PARP inhibition in cancer therapy
- Phagocytosis and Immune Regulation
- DNA Repair Mechanisms
- Signaling Pathways in Disease
- Erythrocyte Function and Pathophysiology
- Atherosclerosis and Cardiovascular Diseases
- T-cell and B-cell Immunology
- Lipid Membrane Structure and Behavior
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immunotherapy and Immune Responses
- Calcium signaling and nucleotide metabolism
- Cancer-related Molecular Pathways
- Integrated Circuits and Semiconductor Failure Analysis
- Glycosylation and Glycoproteins Research
Biotechnology Institute Thurgau
2019-2022
University of Konstanz
2017-2022
University of Bern
2020
The post-translational modification poly(ADP-ribosyl)ation (PARylation) plays key roles in genome maintenance and transcription. Both non-covalent poly(ADP-ribose) binding covalent PARylation control protein functions, however, it is unknown how the two modes of crosstalk mechanistically. Employing tumor suppressor p53 as a model substrate, this study provides detailed insights into interplay between unravels its functional significance regulation p53. We reveal that multifunctional...
Abstract The prime function of nucleoli is ribogenesis, however, several other, non-canonical functions have recently been identified, including a role in genotoxic stress response. Upon DNA damage, numerous proteins shuttle dynamically between the nucleolus and nucleoplasm, yet underlying molecular mechanisms are incompletely understood. Here, we demonstrate that PARP1 PARylation contribute to stress-induced nucleolar-nucleoplasmic shuttling key genome maintenance factors HeLa cells. Our...
Abstract Background T cell activation leads to increased expression of the receptor for iron transporter transferrin (TfR) provide required differentiation and clonal expansion that takes place during days after encounter with a cognate antigen. However, cells mobilise TfR their surface within minutes activation, although reason mechanism driving this process remain unclear. Results Here we show transiently increase endocytic uptake recycling upon thereby boosting capacity import iron. We...
T cell activation is immediately followed by internalization of the receptor (TCR). TCR endocytosis required for activation, but mechanisms supporting removal from surface remain incompletely understood. Here we report that linked to clathrin-independent carrier (CLIC) and GPI-enriched endocytic compartments (GEEC) pathway. We show unlike canonical clathrin cargo transferrin or adaptor protein Lat, internalized accumulates in tubules shaped small GTPase Cdc42 Bin/amphiphysin/Rvs (BAR) domain...
T cell activation requires engagement of a cognate antigen by the receptor (TCR) and co-stimulatory signal CD28. Both TCR CD28 aggregate into clusters at plasma membrane activated cells. While role clustering in has been extensively investigated, little is known about how contributes to signalling. Here, we report that upon triggering, BAR-domain protein sorting nexin 9 (SNX9) recruited immunological synapse. Using three-dimensional correlative light electron microscopy, show SNX9 generates...