A5080663809- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Ion Channels and Receptors
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Pain Mechanisms and Treatments
- Epigenetics and DNA Methylation
- Neuropeptides and Animal Physiology
- Reproductive System and Pregnancy
- Renin-Angiotensin System Studies
- Lysosomal Storage Disorders Research
- Pharmacological Effects of Natural Compounds
- Renal and related cancers
- Protein Interaction Studies and Fluorescence Analysis
- Tryptophan and brain disorders
- Immunotherapy and Immune Responses
- Ion channel regulation and function
- Toxoplasma gondii Research Studies
- Animal Genetics and Reproduction
- Nuclear Structure and Function
- Clusterin in disease pathology
- Neuroendocrine regulation and behavior
- Receptor Mechanisms and Signaling
- RNA Interference and Gene Delivery
Ottawa Hospital Research Institute
2011-2019
Ottawa Hospital
2011-2019
Washington University in St. Louis
2015-2018
Kent State University
2009-2015
University of Ottawa
2011
National Institute of Immunology
2007
Injury, inflammation, and nerve damage initiate a wide variety of cellular molecular processes that culminate in hyperexcitation sensory nerves, which underlies chronic inflammatory neuropathic pain. Using behavioral readouts pain hypersensitivity induced by angiotensin II (Ang II) injection into mouse hindpaws, our study shows activation the type 2 Ang receptor (AT2R) cell-damage-sensing ion channel TRPA1 are required for peripheral mechanical sensitization male female mice. However, we...
Abstract To delineate the functional aspects of zona pellucida (ZP) glycoproteins during fertilization in human, present study, fluorochrome‐conjugated Escherichia coli ( E. )‐ and baculovirus‐expressed recombinant human ZP glycoprotein‐2 (ZP2), ‐3 (ZP3), ‐4 (ZP4) were employed. In an immunofluorescence assay, capacitated sperm exhibited binding ZP3 as well ZP4 to either acrosomal cap or equatorial region whereas acrosome‐reacted failed show any cap. Using double labeling experiments,...
Mammalian sperm contain the serine/threonine phosphatases PP1γ2 and PP2A. The role of is relatively well studied. Here we confirm presence PP2A in show that it undergoes marked changes methylation (leucine 309), tyrosine phosphorylation (tyrosine 307) catalytic activity during epididymal maturation. Spermatozoa isolated from proximal caput, distal caput caudal regions epididymis equal immuno-reactive amounts Using demethyl sensitive antibodies methylated at its carboxy terminus but not...
Clusterin (CLU) is known as an extracellular chaperone for proteins under stress, thus preventing them from aggregation and precipitation. We showed herein that CLU, expressed by principal cells of the mouse caput epididymis, was present in high amounts lumen. In cauda CLU bound tightly to sperm head surface its amount on total similar bathing luminal fluid. both immotile motile caudal epididymal sperm, localized over entire except at convex ridge, although population, immunofluorescence...
Sulfogalactosylglycerolipid (SGG) is the major sulfoglycolipid of male germ cells. During spermatogenesis, apoptosis occurs in >50% total Sertoli cells phagocytose these apoptotic and degrade their components using lysosomal enzymes. Here we demonstrated that SGG was a physiological substrate arylsulfatase A (ARSA). accumulated Arsa(-/-) mice, at 8 months age, this buildup led to swelling other cellular abnormalities typical storage disorder. This disorder likely compromised cell functions,...
Bone metastasis in breast, prostate and lung cancers often leads to chronic pain, which is poorly managed by existing analgesics. The neurobiological mechanisms that underlie pain associated with bone-metastasized are not well understood, but sensitization of peripheral nociceptors tumor microenvironment factors has been demonstrated be important. Parathyroid hormone-related peptide (PTHrP) highly expressed breast cancers, critical growth proliferation these tumors the bone microenvironment....
Two isoforms of phosphoprotein phosphatase 1, PPP1CC1 and PPP1CC2, are translated from alternatively spliced transcripts a single gene, Ppp1cc, differ only at their extreme C-termini. While expression is almost ubiquitous, PPP1CC2 largely restricted to testicular germ cells mature spermatozoa. Targeted deletion Ppp1cc leads sterility −/− males due combination gross structural defects in developing spermatids resulting apoptosis faulty spermiation. Because the PP1 isoform that demonstrates...
Abstract Cultures of Sertoli cells isolated from 20-day-old mice are widely used in research as substitutes for adult cell cultures. This practice is based on the fact that cease to proliferate and become mature vivo by 16 20 days after birth. However, it important verify whether cultured derived do not ex they have same properties cells. Herein we described an isolation/culture method 10-week-old with > 90% purity. Properties these were then compared those (also purity). By counting,...
ABSTRACT Peripheral nerve damage initiates a complex series of cellular and structural processes that culminate in chronic neuropathic pain. Our study defines local angiotensin signaling via activation the Angiotensin II (Ang II) type-2 receptor (AT2R) on macrophages as critical trigger An AT2R-selective antagonist attenuates neuropathic, but not inflammatory pain hypersensitivity mice, requires cell damage-sensing ion channel transient potential family-A member-1 (TRPA1). Mechanical cold...
Two isoforms of protein phosphatase 1, PP1γ1 and PP1γ2, are translated from alternatively spliced transcripts a single gene, Ppp1cc, differ only at their extreme C-termini. While expression is nearly ubiquitous, PP1γ2 largely restricted to testicular germ cells mature spermatozoa. Targeted deletion Ppp1cc leads sterility -/- males due the apoptotic demise structurally defective developing spermatids faulty spermiation. Because PP1 isoform that demonstrates high-level in wild-type meiotic...
The enzymes PP2A and PP1γ2 are the two major serine/threonine phosphatases in mammalian spermatozoa. is implicated diverse cellular functions. In this study, we confirm presence of spermatozoa by western blot show that it carboxy methylated (Lys 309) reciprocally tyrosine phosphorylated (Tyr 307). Mammalian acquire motility their ability to fertilize eggs during transit through epididymis. immotile sperm from late caput region epididymis with negligible phosphorylation whereas motile cauda...