Inorganic enzyme? Ceria nanoparticles exhibit unique oxidase-like activity at acidic pH values. These redox catalysts can be used in immunoassays (ELISA) when modified with targeting ligands (see picture; light blue and yellow structures are attached ligands). This modification allows both for binding detection by the catalytic oxidation of sensitive colorimetric dyes (e.g. TMB).

Cerium oxide nanoparticles (nanoceria) have shown great potential as antioxidant and radioprotective agents for applications in cancer therapy. Recently, various polymer-coated nanoceria preparations been developed to improve their aqueous solubility allow surface functionalization of these nanoparticles. However, the interaction with cells, uptake mechanism, subcellular localization are poorly understood. Herein, we engineered cerium different charges (positive, negative, neutral) studied...

Monodisperse magnetic nanoparticles conjugated with virus-surface-specific antibodies self-assemble in the presence of specific viral particles to create supramolecular structures enhanced properties, as detected by resonance methods (NMR/MRI). The observed relaxation changes that occur upon viral-induced assembly allowed for highly sensitive and selective detection a virus complex biological media. developed method was shown specifically detect adenovirus-5 herpes simplex virus-1 at...

Biocompatible dextran-coated nanoceria (cerium oxide nanoparticles) are found to exhibit excellent, pH-dependent antioxidant properties (see image). They provide cytoprotection from free radicals normal cells but not cancer cells, and thus have potential applications in anti-inflammatory coatings for prosthetic devices tissue engineering improved therapeutics. Supporting information this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2296/2008/smll200700824_s.pdf...

A biocompatible, multimodal, and theranostic functional iron oxide nanoparticle is synthesized using a novel water-based method exerts excellent properties for targeted cancer therapy, optical magnetic resonance imaging. For the first time, facile, modified solvent diffusion used co-encapsulation of both an anticancer drug near-infrared dyes. The resulting folate-derivatized theranostics nanoparticles could allow optical/magnetic imaging killing folate-expressing cells.

Herein we describe the design and synthesis of a folate–doxorubicin conjugate with activatable fluorescence cytotoxicity. In this study discovered that cytotoxicity doxorubicin are quenched (OFF) when covalently linked folic acid. Most importantly, is designed disulfide bond linking targeting folate unit cytotoxic doxorubicin, targeted prodrug obtained becomes activated (ON) within cell by glutathione-mediated dissociation nuclear translocation, showing enhanced cellular toxicity. our novel...

The reliable and sensitive detection of cancer-specific biomarkers is important for the diagnosis treatment cancer. Hence, these has to be reliably rapidly performed in diverse settings. A limitation conventional biomarker-screening method enzyme-linked immunosorbent assay (ELISA) employment labile components, such as hydrogen peroxide horseradish peroxidase. Previously, we reported that nanoceria able oxidize various colorimertic dyes at acidic pH, 3,3′,5,5′-tetramethylbenzydine (TMB)...

Abstract Anorganisches Enzym? Ceroxidnanopartikel zeigen einzigartige Oxidaseaktivität bei niedrigen pH‐Werten. Diese Redoxkatalysatoren können in Immunassays (ELISA) verwendet werden, wenn sie mit geeigneten Liganden modifiziert sind (siehe Bild; hellblau, gelb: Nanopartikel Liganden). Modifizierung ermöglicht sowohl die Bindung als auch Detektion durch katalytische Oxidation empfindlicher kolorimetrischer Farbstoffe (z. B. TMB). magnified image

Acknowledging the need for development of fast and sensitive bacterial detection methods, we functionalized superparamagnetic iron oxide nanoparticles to identify Mycobacterium avium spp. paratuberculosis (MAP), through magnetic relaxation. Our results indicate that MAP nanoprobes (1) bind specifically MAP, (2) can quantify target quickly in milk blood with high sensitivity, (3) are not susceptible interferences caused by other bacteria. The use described nanosensors identification...

We report the production of poly(lactic-co-glycolic acid) nanoparticles that encapsulate photosensitizer meso-tetraphenylporpholactol. These are stable and nonphototoxic upon systemic administration. Upon cellular internalization, is released from nanoparticle becomes highly phototoxic. Irradiation with visible light results in cell-specific killing several cancer cell lines. Importantly, vivo experiments show complete eradication cancers mouse models. The concept photosensitizers selective...

The development of bacterial resistant strains is a global health concern. Designing antibiotics that limit the rise pathogenic resistance essential to efficiently treat infections. Self-assembling amphiphilic molecules are an intriguing platform for treatment pathogens because their ability disrupt membranes and function as drug nanocarriers. We have designed cationic peptide amphiphiles (PAs) can form micelles, nanofibers, twisted ribbons with aim understanding antimicrobial activity at...

Herein we report a novel gadolinium-encapsulating iron oxide nanoparticle-based activatable NMR/MRI nanoprobe. In our design, Gd-DTPA is encapsulated within the poly(acrylic acid) (PAA) polymer coating of superparamagnetic nanoparticle (IO-PAA), yielding composite magnetic nanoprobe (IO-PAA-Gd-DTPA) with quenched longitudinal spin–lattice relaxation (T1). Upon release complex from nanoprobe's polymeric in acidic media, an increase T1 rate (1/T1) was observed, indicating dequenching...

Bacteria rapidly evolve mechanisms to become resistant antibiotics. Therefore, identifying an effective antibiotic or antibacterial agent and administering it at concentrations that will successfully prevent bacterial growth (antimicrobial susceptibility) is critical for health care decision making vital the battle against multi-drug-resistant bacteria. Currently, determination of antimicrobial susceptibility requires least 24 h. Herein, we describe a nanoparticle-based assay based on...

A simple strategy for detection of glutathione (GSH) in physiologically relevant concentrations is reported by manipulating the electron transfer pathways surface-modified quantum dots. The probe consisted fluorescent CdS:Mn/ZnS core/shell dots (Qdot) properly conjugated to dopamine through CS2-assisted zero-length covalent coupling. Qdots exhibited bright yellow-orange emission at ∼ 592 nm, which could be attributed 4T1 → 6A1 transition Mn2+ ions Qdot core. fluorescently dark (OFF state)...

Monodisperse, water-soluble dextran-coated iron oxide (Fe3O4) nanorods were synthesized using a facile and scalable approach. Our room temperature method involves the mixing of an acidic solution salts with basic ammonium hydroxide to facilitate initial formation crystals. The stability, crystallinity, shape these depends on time addition dextran, as well degree purity polymer. as-synthesized exhibit unique magnetic properties, including superparamagnetic behavior high spin−spin water...

Herein, we report the use of a theranostic nanocarrier (Folate-HBPE(CT20p)) to deliver therapeutic peptide prostate cancer tumors that express PSMA (folate hydrolase 1). The (CT20p) targets and inhibits chaperonin-containing TCP-1 (CCT) protein-folding complex, is selectively cytotoxic cells, non-toxic normal tissue. With delivery CT20p cells via PSMA, dual level specificity achieved: (1) selective targeting PSMA-expressing tumors, (2) specific cytotoxicity with minimal toxicity cells....

We have designed a set of multifunctional and multicoordinating polymer ligands that are optimally suited for surface functionalizing iron oxide potentially other magnetic nanoparticles (NPs) promoting their integration into biological systems. The amphiphilic polymers prepared by coupling (via nucleophilic addition) several amine-terminated dopamine anchoring groups, poly(ethylene glycol) moieties, reactive groups onto poly(isobutylene-alt-maleic anhydride) (PIMA) chain. This design greatly...

Despite significant efforts to improve glioblastoma multiforme (GBM) treatment, GBM remains one of the most lethal cancers. Effective treatments require sensitive intraoperative tumor visualization and effective postoperative chemotherapeutic delivery. Unfortunately, diffusive infiltrating nature limits detection tumors, current methods limit complete resection. In addition, although chemotherapy is often used eliminate any cancerous tissue remaining after surgery, drugs do not effectively...

Considering the increased incidence of bacterial infections and emergence multidrug resistant bacteria at global level, we designed superparamagnetic iron oxide nanoparticles as nanosensors for assessment antimicrobial susceptibility through magnetic relaxation. In this report, demonstrate that nanosensors, either dextran-coated supplemented with Con A or silica-coated conjugated directly to A, can be used fast (1) quantification polysaccharides, (2) metabolic activity (3) determination in...