Abstract Aim To verify, under the scanning electron microscope (SEM), influence of irrigation time with ethylenediaminetetraacetic acid (EDTA) and sodium hypochlorite (NaOCl) on intracanal smear layer removal. Methodology Twenty‐one extracted human permanent teeth single straight root canals were included. The instrumented and, at end preparation, irrigated 3 mL 15% EDTA, followed by 1% NaOCl for 1 min (group 1), 2), 5 3). in group 4 (control) did not receive final irrigation. sectioned...

Abstract Aim To evaluate the influence of mineral trioxide aggregate (MTA) on apexification and periapical healing teeth in dogs with incomplete root formation previously contaminated canals to verify necessity employing calcium hydroxide paste before using MTA. Methodology Twenty premolars from two 6‐month old were used. After access complete removal pulp, canal systems remained exposed oral environment for 2 weeks. Canal preparation was then carried out Hedström files, under irrigation 1%...

To evaluate the effectiveness of various storage media at 5 °C for maintaining viability human periodontal ligament fibroblasts (PDLF).Plates with PDLF were soaked in recently prepared Hank's balanced salt solution (HBSS), skimmed milk, whole Save-A-Tooth(®) system's HBSS (Save), natural coconut water, industrialized water or tap (negative control) 3, 6, 24, 48, 72, 96 and 120 h. Minimum essential medium (MEM) 37 served as positive control. PDL cell was determined by MTT assay. Data...

Abstract Aim To evaluate the influence of renewing calcium hydroxide paste on apexification and periapical healing teeth in dogs with incomplete root formation previously contaminated canals. Methodology Forty premolars from four 6‐month‐old were used. After access to canals complete removal pulp, canal systems remained exposed oral environment for 2 weeks. Canal preparation was then carried out using Hedströem files, under irrigation 1% sodium hypochlorite, 1 mm short radiographic apex....

To evaluate connective tissue reactions to iRoot SP (Innovative Bioceramics, Vancouver, BC, Canada), mineral trioxide aggregate (MTA) Fillapex (FLPX) (Angelus Soluções Odontológicas, Londrina, Brazil), DiaRoot Bioaggregate (DiaDent Group International, Burnaby, Canada) and white MTA (Angelus, Brazil) in Wistar rats.A total of 128 dentine tubes filled with the materials 32 empty (control) were implanted into rats. After 7, 15, 30 90 days (n = 8 per period), animals euthanized, tissues...

To evaluate nanoparticulate calcium carbonate (NPCC) using transmission electron microscopy and the effects of NPCC addition to MTA in regard setting time, dimensional change, compressive strength, solubility pH.The experimental groups were G1 (MTA), G2 (MTA with 5% NPCC) G3 10% NPCC). The tests followed ISO ADA standards. specimens change strength measured immediately after setting, 24 h 30 days. In test, rings filled cement weighed pH was data analysed ANOVA, Tukey's Kruskal-Wallis (α =...

Abstract – Hank’s balanced salt solution (HBSS) is recommended for the storage of avulsed teeth. The objective this study was to evaluate if HBSS time influences its ability maintain viability human periodontal ligament fibroblasts (PDLF) by analysis cell metabolic function using MTT assay. PDLF were kept at 20°C 3, 6, 24, 48, 72, 96 and 120 h in recently prepared (HBSS), stored 6 months (HBSS M), 12 Save‐A‐Tooth system’s (Save). Minimum essential medium (MEM) 37°C tap water served as...

Natural resources, such as coconut water, propolis, and egg whites, have been examined possible storage media for avulsed teeth. However, there is a lack of research focused on the efficacy these three products together compared with Hank's balanced salt solution milk. The aim this study was to evaluate capacity seven maintain viability human periodontal ligament fibroblasts (PDLFs).PDLFs were kept at 5°C 20°C, in skimmed milk (SMilk), whole (WMilk), recently prepared (HBSS), Save-A-Tooth®...

Regenerative endodontic procedure (REP) has been proposed as a new approach to treat immature permanent teeth. However, materials used in REP for root canal disinfection or cervical sealing may induce tooth discoloration. Objectives To assess crown's color after intracanal treatment with triple antibiotic paste (TAP) calcium hydroxide (CH); glass ionomer cement (GIC) mineral trioxide aggregate (MTA); and bleaching carbamide peroxide. Material Methods After pulp removal spectrophotometer...

ABSTRACT This study evaluated the bond strength (BS) and adhesive interface of four endodontic sealers to root canal dentine, before, after immersion in phosphate buffered saline (PBS) simulate an vivo environment. Eighty roots were instrumented using ProTaper rotatory files, under irrigation with 17% EDTA 1% NaOCl. Posteriorly divided into groups ( n = 20) according sealer used: Endofill, AH Plus, Sealapex, MTA Fillapex. Each group was two subgroups 10) stored at 37°C immersed water for 7...

Abstract Background/Aim Natural resources, such as coconut water, propolis, and egg whites, have been examined possible storage media for avulsed teeth. However, there is a lack of research focused on the efficacy these three products together compared with Hank's balanced salt solution milk. The aim this study was to evaluate capacity seven maintain viability human periodontal ligament fibroblasts (PDLFs). Material methods PDLFs were kept at 5°C 20°C, in skimmed milk (SMilk), whole (WMilk),...

Revascularization of immature teeth with necrotic pulps traditionally involves the use triple antibiotic paste, which may sometimes lead to undesirable complications. The objective this study was assess tissue repair in dog apical periodontitis subjected revascularization, comparing two different pastes used for root canal disinfection. Apical induced 30 premolars. Teeth were randomly divided into three experimental groups: canals filled paste (n = 10); 1% propolis and no medication 10). An...

Abstract Aim To evaluate ex vivo the capacity of Tri Auto ZX to locate apical foramen during root canal retreatment. Methodology The canals 62 maxillary and mandibular canines were prepared a length 1 mm short foramen, an size 35 using 1% sodium hypochlorite as irrigant. Once prepared, each tooth was measured directly 15 K‐Flexofile introduced in until tip visible at foramen. After file removed, its recorded precision 0.01 calliper. These direct lengths (DL) became ‘gold standard’ for...

To analyse the influence of exposure mineral trioxide aggregate (MTA) with and without calcium chloride (CaCl2 ) to phosphate-buffered saline (PBS) on push-out bond strength, over different experimental periods.One hundred twenty dentine discs standardized cavities were filled MTA 10% CaCl2 . The specimens randomly divided as follows (n = 30): (G1) in contact a moistened cotton pellet, (G2) immersed PBS, (G3) + pellet (G4) PBS. samples stored for 3, 28 60 days. strengths measured Instron...

Abstract Aim To evaluate the capacity of ROOT ZX II to locate apical foramen and control extent rotary instrumentation. Methodology Sixty‐five extracted human single rooted teeth were selected measured directly using a size 15 K‐Flexofile introduced in canal until tip was visible at major (direct length, DL). The then electronically (EL1) with when used passively, that is without rotation. test auto reverse function, root canals instrumented nickel titanium instruments. Instrumentation...

The aim is to verify, under scanning electron microscopy, the occurrence of apical root resorption in anterior and posterior teeth with chronic radiographically visible periapical lesions. Thirty-two were extracted, thirds (n = 39) removed, prepared analyse external surface. All specimens presented irregular areas different depths, located mainly around foramen. Teeth lesions present surface depths Root resorptions may show configurations destroy structure more or less extensively.