A5101415899- CRISPR and Genetic Engineering
- Hungarian Social, Economic and Educational Studies
- Advanced biosensing and bioanalysis techniques
- Metal complexes synthesis and properties
- Bacterial Genetics and Biotechnology
- Magnetism in coordination complexes
- Cellular transport and secretion
- Antifungal resistance and susceptibility
- RNA and protein synthesis mechanisms
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Microbial Natural Products and Biosynthesis
- Regional Development and Policy
- Mycorrhizal Fungi and Plant Interactions
- Fungal Infections and Studies
- Prion Diseases and Protein Misfolding
- Lanthanide and Transition Metal Complexes
- SARS-CoV-2 and COVID-19 Research
- Food composition and properties
- Cystic Fibrosis Research Advances
- Phosphorus and nutrient management
- Neurological diseases and metabolism
- Heavy metals in environment
- Bacteriophages and microbial interactions
- Plant chemical constituents analysis
Magyar Agrár- és Élettudományi Egyetem
2002-2024
HUN-REN Research Centre for Natural Sciences
2017-2023
Institute of Molecular Life Sciences
2017-2023
Centre for Agricultural Research
2023
University of Szeged
2011-2020
University of Debrecen
2019-2020
MTA-SZTE Research Group on Artificial Intelligence
2019
University of California, Santa Cruz
2018
University of Tsukuba
2017-2018
Hungarian Academy of Sciences
2013-2017
In highly polarized cells such as neurons, compartmentalization of mRNA and local protein synthesis enables remarkably fast, precise, responses to external stimuli. These are important for neuron growth cone guidance, synapse formation, regeneration following injury. Because an altered spatial distribution can result in mental retardation or neurodegenerative diseases, subcellular transcriptome analysis neurons could be a useful tool studying these conditions, but current techniques, situ...
Abstract This study focused on the physical, chemical and microbiological characteristics of soils from different grasslands in Budapest area. Our aim was to assess influence anthropogenic activities these by comparing three types site: urban, green‐urban non‐urban. From sites, a total 72 topsoil samples were collected analysed. The results indicated that many properties varied greatly between site. Urban had significantly higher pH, carbonate salt content, but smaller organic carbon content...
The efficacies of guide RNAs (gRNAs), the short RNA molecules that bind to and determine sequence specificity Streptococcus pyogenes Cas9 nuclease, mediate DNA cleavage vary dramatically.Thus, selection appropriate target sites, hence spacer sequence, is critical for most applications.Here, we describe a simple, unparalleled method experimentally pre-testing efficiencies various gRNAs targeting gene.The explores NHEJcloning, genomic integration GFP-expressing plasmid without homologous arms...
In the present study, in vitro antifungal activities of five antipsychotic drugs (i.e., chlorpromazine hydrochloride, CPZ; trifluoperazine TPZ; amantadine hydrochloride; R-(-)-deprenyl and valproic acid sodium salt) conventional amphotericin B, AMB; caspofungin, CSP; itraconazole; terbinafine, TRB voriconazole, VRC) were investigated broth microdilution tests against four clinical environmental Scedosporium Pseudallescheria isolates. When used alone, phenothiazines CPZ TPZ exerted remarkable...
Abstract Cannibalistic necrophagy is rarely observed in social hymenopterans, although a lack of food could easily favour such behaviour. One the main supposed reasons for rarity that eating nestmate corpses carries risk rapid spread pathogens or parasites. Here we present an experimental laboratory study on behaviour indicating consumption ant Formica polyctena . We examined whether starvation and fungal infection level affects occurrence cannibalistic necrophagy. Our results showed ants...
Summary A reliable identification method was developed for three closely related Curvularia species, which are frequently isolated from human keratomycoses. Since the traditionally used morphological and increasingly internal transcribed spacer (ITS)‐based molecular proved to be insufficient discern C. australiensis, hawaiiensis spicifera , other targets, such as β‐tubulin, translation elongation factor 1‐α nuclear ribosomal intergenic ( IGS ), were tested. Among them, use of highly...
Members of the Scedosporium apiospermum species complex are second most frequently isolated pathogens after Aspergillus fumigatus from cystic fibrosis (CF) patients with fungal pulmonary infections. Even so, main risk factors for infection unrevealed. According to previous studies, bacterial infections might reduce a infection, but an antibacterial therapy may contribute airway colonization by several pathogens. Furthermore, corticosteroids, which often used lung inflammation in children and...
Abstract Biologically active compounds are key-important ingredients of tomato fruits, especially those produced by organic agricultural practices. Being biologically effective soil treatments, microbial inoculants can improve quality through a variety mechanisms. Their activity influence properties and plant development, thereby determining the quantity crop. However, little research was done on how these affect nutritional fruit antioxidant status fruits. This study investigated lycopene...
The nuclease domain of colicin E7 metallonuclease (NColE7) contains its active centre at the C-terminus. mutant ΔN4-NColE7-C* - where four N-terminal residues including positively charged K446, R447 and K449 are replaced with eight from GST tag is catalytically inactive. crystal structure this demonstrates that overall fold very similar to native NColE7 structure. This implicates stabilizing effect remaining sequence on C-terminal catalytic site essential role deleted in mechanism catalyzed...
While the majority of children infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) display mild or no symptoms, rare individuals develop disease presenting multisystem inflammatory (MIS-C). The reason for variable clinical manifestations is not understood. Here, we carried out TCR sequencing and conducted comparative analyses repertoires between MIS-C (n = 12) 8) COVID-19. We compared these unexposed (samples collected pre-COVID-19 pandemic: n Adaptive Biotechnologies...
In recent years, Scedosporium species have been more commonly recognized from severe, difficult-to-treat human infections, such as upper respiratory tract and pulmonary infections. To select an appropriate therapeutic approach for these infections is challenging, because of the observed resistance causative agents to several antifungal drugs. Therefore, find a novel strategy treatment in vitro effect mucolytic agent, N-acetyl-L-cysteine its combinations with conventional antifungals were...
Streptococcus pyogenes Cas9 (SpCas9) has been employed as a genome engineering tool with promising potential within therapeutics. However, its off-target effects present major safety concerns for applications requiring high specificity. Approaches developed to date mitigate this effect, including any of the increased-fidelity (i.e., high-fidelity) SpCas9 variants, only provide efficient editing on relatively small fraction targets without detectable off-targets. Upon addressing problem, we...
Mosses are traditionally used as ornamental plants, especially in Japan, USA, England (moss gardens) and Germany, French (green roofs). In shadow areas (where the members of Poaceae usually cannot grow well), mosses potentially use “grass”. The stocks moss colonies maintain optimal microclimate decrease desiccation soils (like mulch). Additionally, evergreen, attractive all year, during winter. our study, 18 species were propagated by fragments (as mixture, with 16 species) transplantation...
The metallonuclease colicin E7 is a member of the HNH family endonucleases. It serves as bacterial toxin in Escherichia coli, protecting host cell from other related bacteria and bacteriophages by degradation their chromosomal DNA under environmental stress. Its cell-killing activity attributed to nonspecific nuclease domain (NColE7), which possesses catalytic ββα-type metal ion-binding motif at its C-terminus. Mutations affecting positively charged amino acids N-terminus NColE7 (444-576)...
The investigation of the linkage isomers biologically essential and kinetically labile metal complexes in aqueous solutions poses a challenge, as these microspecies cannot be separately studied. Therefore, derivatives are commonly used to initially determine stability or spectral characteristics at least one isomers. Here we directly detect isomers, describe ion coordination sphere, speciation thermodynamic parameters by synergistic application temperature dependent EPR CD spectroscopic...
The use of microbial inoculums is a part sustainable agricultural practices. Among various bioeffectors, the phosphorus-mobilizing bacteria are frequently used.
 objective this study to investigate effect some industrial biofertilizer inoculums, containing P-mobilizing on quantity and quality parameters tomato fruits. Spore-forming Bacillus amyloliquefaciens FZB42 (Rhizovital) as single combinations with other strains (Biorex) were applied Solanum lycopersicon Mill. var. Mobil test...
1990 után a kelet-közép-európai országok helyzete geopolitikai és geoökonómia szempontból megváltozott. A rendszerváltozásnak köszönhetően újra annak az Európának részeivé váltak, melyből második világháború Szovjetunió kiszakította őket. térség államai rendszerváltozás utáni első évtizedben európai integráció felé fordultak. 2004-ben megvalósult Európai Unió keleti bővítése Európa újraegyesítése. tagság bő évtizede elmondható, hogy kelet-közép gazdasági számos egyéb profitáltak tagságból....
SUMMARY Protection of propagation material several medicinal plants in the frame a genebank project is being carried out our department. In present work we have examined changes germination capacity Foeniculum vulgare Mill. (Apiaceae) different origins. The four years' results revealed reactions tested seeds respect capacity. effects storage temperature, duration, ripening phase, intraspecific taxa and vegetation year were detail.