A5022587293- Prion Diseases and Protein Misfolding
- DNA Repair Mechanisms
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Microtubule and mitosis dynamics
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- RNA Research and Splicing
University of Oregon
2021-2024
California Polytechnic State University
2018
Abstract Transfer RNAs (tRNAs) contain dozens of chemical modifications. These modifications are critical for maintaining tRNA tertiary structure and optimizing protein synthesis. Here we advance the use Nanopore direct RNA-sequencing (DRS) to investigate synergy between that known stabilize structure. We sequenced 42 cytosolic isoacceptors from wild-type yeast five tRNA-modifying enzyme knockout mutants. data permitted comprehensive analysis three neighboring conserved in T-loops:...
In fluctuating environments, switching between different growth strategies, such as those affecting cell size and proliferation, can be advantageous to an organism. Trade-offs arise, however. Mechanisms that aberrantly increase or proliferation-such mutations chemicals interfere with regulatory pathways-can also shorten lifespan. Here we report a natural example of how the interplay lifespan epigenetically controlled. We find highly conserved RNA-modifying enzyme, pseudouridine synthase...