A5012502103- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Organ Transplantation Techniques and Outcomes
- Genomics and Rare Diseases
- Transplantation: Methods and Outcomes
- Renal Transplantation Outcomes and Treatments
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Pancreatic function and diabetes
- Virus-based gene therapy research
- Metabolism and Genetic Disorders
- HIV, Drug Use, Sexual Risk
- Misinformation and Its Impacts
- Vaccine Coverage and Hesitancy
- International Human Rights and Reproductive Law
- Health Literacy and Information Accessibility
- Viral Infections and Immunology Research
- Social Media in Health Education
- Liver physiology and pathology
- Hepatitis B Virus Studies
- Adipose Tissue and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Mitochondrial Function and Pathology
- Bone health and osteoporosis research
Wilhelmina Children's Hospital
2018-2025
University Medical Center Utrecht
2018-2024
Hôpital Lariboisière
2021
Utrecht University
2020
Maastricht University
2016
Abstract Prime editing is a versatile genome-editing technique that shows great promise for the generation and repair of patient mutations. However, some genomic sites are difficult to edit optimal design prime-editing tools remains elusive. Here we present fluorescent prime enrichment reporter (fluoPEER), which can be tailored any target site. This system rapidly faithfully ranks efficiency guide RNAs (pegRNAs) combined with editor variant. We apply fluoPEER instruct correction pathogenic...
We live in an age where the sharing of scientific findings and ideas is no longer confined to people with access academic libraries or journals. Social media have permitted for knowledge be shared unprecedented speed magnitude. This has made it possible research a greater impact rapidly implemented society. However, spread unfiltered, unreferenced, non-peer-reviewed articles through social comes dangers as well. In this perspective article, we aim address both possibilities pitfalls...
For liver transplantations, human leukocyte antigen (HLA) matching is not routinely performed because observed effects have been inconsistent. Nevertheless, long-term transplantation outcomes remain suboptimal. The availability of a more precise HLA-matching algorithm, Predicted Indirectly Recognizable HLA Epitopes II (PIRCHE-II), now enables robust assessment the association between and outcomes. We single-center retrospective cohort study 736 patients. Associations PIRCHE-II HLAMatchmaker...
Aminoacyl-tRNA synthetases (aaRS) are ubiquitously expressed enzymes responsible for ligating amino acids to their cognate tRNA molecules through an aminoacylation reaction. The resulting aminoacyl-tRNA is delivered ribosome elongation factors participate in protein synthesis. Seryl-tRNA synthetase (SARS1) one of the cytosolic aaRSs and catalyzes serine attachment tRNASer. SARS1 deficiency has already been associated with moderate intellectual disability, ataxia, muscle weakness, seizure...
Abstract Aminoacyl-tRNA synthetases (ARSs) couple tRNAs with their corresponding amino acids. While ARSs can bind structurally similar acids, extreme specificity is ensured by subsequent editing activity. Yet, we found that upon isoleucine (I) restriction, healthy fibroblasts consistently incorporated valine (V) into proteins at codons, resulting from misacylation of tRNAIle wildtype IARS1. Using a dual-fluorescent reporter translation, supplementation could fully compensate for depletion...
Abstract Prime editing is a versatile genome-editing technique. However, some genomic sites remain difficult to edit and optimal design of prime-editing tools elusive. We present fluorescent prime enrichment reporter (fluoPEER), which can be tailored any target site. This system rapidly faithfully ranked the efficiency guide RNAs (pegRNAs) editor protein, including novel variants with flexible protospacer adjacent motif (PAM) recognition. applied fluoPEER instruct correction pathogenic in...
Tens of thousands women and girls are trafficked from mainly poorer to richer regions Europe forced into prostitution. Traffickers abuse the poor socio-economic situation young in these exploit them Sex trafficking poses a huge impact on society. Besides economical side – sanctions for not complying with certain anti-trafficking efforts or health care costs victims political- social is drastic. Social huge: during their they face extreme violence, coercion, rape, which leave both...