The spatial resolution of conventional optical microscopy is limited by diffraction to transverse and axial resolutions about 250 nm, but localization point sources, such as single molecules or fluorescent beads, can be achieved with a precision 10 nm better in each direction. Traditional approaches two dimensions enable high only for thin in-focus layer that typically much less than the depth cell. This precludes, example, super-resolution extended three-dimensional biological structures...

The localization of point sources in optical microscopy enables nm-precision imaging single-molecules and biological dynamics. We report a new method using twin Airy beams that yields precise 3D with the key advantages extended depth range, higher throughput, potential for emitter densities than are possible other techniques. A precision better 30 nm was achieved over range excess 7 μm 60×, 1.4 NA objective. An illustrative application to extended-depth-range blood-flow live zebrafish is...

We propose an analytical pupil phase function employing cropped secondary astigmatism for extended-depth nanoscale 3D-localization microscopy. The provides high localization precision in all three dimensions, which can be maintained over extended axial ranges, customizable up to two orders of magnitude relative the conventional, diffraction-limited imaging. This enables, example, capturing dynamics within a whole cell. flexibility and simplicity implementation proposed make its adoption...

A new single-aperture 3D particle-localization and tracking technique is presented that demonstrates an increase in depth range by more than order of magnitude without compromising optical resolution throughput. We exploit the extended depth-dependent translation Airy-beam PSF for localization over volume a single snapshot. The applicable to all bright-field fluorescence modalities particle tracking, ranging from super-resolution microscopy through fluorescent beads endogenous particles...

A previously described optical technique for enhancing interference colour contrast in polarized light microscopy is adapted quantitative image analysis. This based on a linear relationship intensity vs. molecular birefringence and degree of phase alignment, derived using nematic liquid crystal approximations, verified experimentally. The analysis illustrated section the marginal rib dogfish egg case.

Due to the necessity of making a series random adjustments after mode-locking in most experiments for preparing soliton molecules, repeatability preparations remains challenge. Here, we introduce novel all-polarization-maintaining erbium-doped fiber laser, which is modified version Figure-9 laser. This laser can stably output molecules without any additional adjustment once self-starts. Moreover, it achieve transition from molecule state state, and then multi-pulse by reducing pumping power....

Abstract Three-dimensional (3D) imaging of individual atoms is a critical tool for discovering new physical phenomena and developing technologies in microscopic systems. However, the current single-atom-resolved 3D methods are limited to static circumstances or shallow detection range. Here, we demonstrate generic dynamic method track extensive motion single ions by exploiting engineered point-spread function (PSF). We show that image ion can be into helical PSF, thus enabling...

A compact and robust design of an optical lattice clock with high stability accuracy is needed for the implementation clocks operating on space station. Here, we present proof-of-concept model a 87Sr constructed at National Time Service Center. The different from that laboratorial in it focuses aspects compactness robustness, such as modular design, components, low power consumption, all-fiber links. physics package placed aluminum alloy rack dimensions 40 × 55 28 cm3. As far know, this most...

Experimental characterization of blood flow in living organisms is crucial for understanding the development and function cardiovascular systems, but there have been no techniques reported snapshot imaging thick samples large volumes with high precision. We combined computational microscopy diffraction-free, self-bending property Airy beams to track fluorescent beads sub-micron precision through an extended axial range (up 600 $\mu$m) within flowing 3 days post-fertilization (dpf) zebrafish...

The world's first space optical clock (SOC) developed in China, which is composed of five subsystems, i.e. an unit, a physics electronic control frequency comb, and ultrastable laser, was successfully launched with the Mengtian laboratory on October 31, 2022, entered into China Space Station (CSS). Compact stable laser key element for operation SOC. unit consists 5 lasers wavelengths 461, 679, 689, 707 813 nm, respectively. With synchronous-tuning-like scheme, high-quality external cavity...

As quantum computing techniques develop rapidly, the security of classical communication, which is usually based on public key encryption algorithm, under great threat. Therefore, a establishment method with physics base demanding, especially for Internet Things devices, where energy and computational power quite limited. In this article, we present flexible continuous-wave cryptography scheme systems. configuration, IoT controller contains narrow linewidth laser as real local oscillator....

Although photon emission microscope (PEM) systems are widely used in integrated circuit failure analysis, there is no known quantitative baseline to assess and compare the overall sensitivity performance of PEM systems. This paper describes a method quantify based on spectral detectivity measurements. It has been applied HgCdTe (MCT) InGaAs also an system change as temperature detector changes. The signal noise ratio image by normal time integration with digital where many frames added up...

Due to the necessity of making a series fine adjustments after mode-locking in most experiments for preparing soliton molecules, repeatability preparations remains challenge. Here, we propose novel all-polarization-maintaining erbium-doped fiber laser that utilizes nonlinear amplifying loop mirror and features linear structure. This can stably output molecules without any additional adjustment once self-starts. It achieve all-optical switching single-pulse operation through changing pumping...

This paper presents studies on the carrier lensing effect of a tapered amplifier in compact cavity-enhanced second harmonic generator. When different injecting currents are applied, carriers depleted to levels depending local optical field intensity, resulting spatial variation refractive index and creating an effective convex lens for amplified laser beam. can significantly reduce mode matching between pump beam cavity, leading degradation generator efficiency. To characterize this provide...

The 3D location and dipole orientation of light emitters provide essential information in many biological, chemical, physical systems. Simultaneous acquisition both types typically requires pupil engineering for localization dual-channel polarization splitting deduction. Here we report a geometric phase helical point spread function simultaneously estimating the position emitters. It has compact simpler optical configuration compared to polarization-splitting techniques yields achromatic...

Despite the wide applications for high-repetition-rate mode-locked fiber lasers, challenges persist in shortening cavity length and coupling collimators most existing techniques. Here, we introduce a novel collimator alignment method demonstrate an all-polarization-maintaining erbium-doped laser that contains nonlinear amplifying loop mirror with repetition rate of 213 MHz. Compared to conventional method, achieve in-situ simplified two-step process. Besides, through comparison spectra from...

The 3D location of light emitters provides essential information in many biological, chemical and physical systems. 4Pi single-molecule localization microscopy (SMLM) being one the state-of-the-art techniques exceptional precision axial direction; however, lateral degrades significantly when emitter is displaced several wavelengths away from mutual focal plane. Here we propose a hybrid system employing type-B microscope engineered point-spread function (PSF), resultant interferometric PSF...

The spectrum of the output pulses from figure-9 laser typically exhibits more distortion than spectra mode-locked lasers based on other saturable absorbers and its intracavity pulses. Here, we demonstrate two with repetition rates 190.6 MHz 92.4 introduce self-designed beam splitter little spectral impact in fiber loop to interference-free By numerically processing these pulses, formation mechanisms specific features are determined, consistent experimental ports. Furthermore, by analyzing...

We report the design and implementation of a new pupil-engineered phase function that enables simple robust 3D localization microscopy with ten-fold extension in depth-of-field. Applications include single-particle tracking, super resolution lab-on-chip. demonstrate its application to vivo mapping blood flow zebrafish.

We describe a novel 3D particle image velocimetry (PIV) technique in microscopy using the wave-front coding method. Unlike conventional stereoscopic PIV, this has an extended depth-of-field (DOF) and requires only single lens detector.

Microendoscope imaging systems suffer from high levels of aberrations, a narrow Field View and short Depth Field. We present technique for enhanced through microendoscope using novel computational approach.

We present a single-aperture 3D particle localisation and tracking technique with vastly increased depth-of-field without compromising optical resolution throughput. Flow measurements in FEP capillary zebrafish blood vessel are demonstrated experimentally.