A5075764409- Inhalation and Respiratory Drug Delivery
- Pesticide Exposure and Toxicity
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Dermatology and Skin Diseases
- COVID-19 Clinical Research Studies
- HIV Research and Treatment
- Immune responses and vaccinations
- Advanced Drug Delivery Systems
- Bacterial Infections and Vaccines
- Bone Tissue Engineering Materials
- Radiation Dose and Imaging
- Viral gastroenteritis research and epidemiology
- Allergic Rhinitis and Sensitization
- Electrohydrodynamics and Fluid Dynamics
- Cholinesterase and Neurodegenerative Diseases
- Radioactive contamination and transfer
- Advancements in Transdermal Drug Delivery
- Neuroscience and Neuropharmacology Research
- Immune Cell Function and Interaction
- vaccines and immunoinformatics approaches
- Immunodeficiency and Autoimmune Disorders
- Radioactive element chemistry and processing
- Clostridium difficile and Clostridium perfringens research
- Antibiotics Pharmacokinetics and Efficacy
Alchem Laboratories (United States)
2020-2024
Nanotherapeutics (United States)
2004-2017
RTI Surgical (United States)
2013
Tulane University
2012
Air Force General Hospital PLA
2012
National Institutes of Health
2011
National Institute of Biomedical Imaging and Bioengineering
2011
Tokyo Medical and Dental University
2010
University of Florida
2001
Effective SARS-CoV-2 vaccines are urgently needed. Although most vaccine strategies have focused on systemic immunization, here we compared the protective efficacy of 2 adjuvanted subunit with spike protein S1: an intramuscularly primed/boosted and primed/intranasally boosted mucosal in rhesus macaques. The intramuscular-alum–only induced robust binding neutralizing antibody persistent cellular immunity systemically mucosally, whereas intranasal boosting nanoparticles, including IL-15 TLR...
Collagen scaffolds have been widely employed as a dermal equivalent to induce fibroblast infiltrations and regeneration in the treatment of chronic wounds diabetic foot ulcers. Cross-linking methods developed address disadvantages rapid degradation associated with collagen-based scaffolds. To eliminate potential drawbacks glutaraldehyde cross-linking, using water soluble carbodiimide developed. In present study, glycosaminoglycan (GAG) hyaluronic acid (HA), was covalently attached an equine...
It is widely believed that protection against acquisition of HIV or SIV infection requires anti-envelope (anti-Env) antibodies, and cellular immunity may affect viral loads but not acquisition, except in special cases. Here we provide evidence to the contrary. Mucosal immunization enhance vaccine efficacy by eliciting protective responses at portals exposure. Accordingly, vaccinated macaques mucosally with HIV/SIV peptides, modified vaccinia Ankara-SIV (MVA-SIV), HIV-gp120-CD4 fusion protein...
The global health community is beginning to understand the burden of norovirus-associated disease, which has a significant impact in both developed and developing countries. Norovirus virus like particle (VLP)-based vaccines are currently under development have been shown elicit systemic mucosal immune responses when delivered intranasally. In present study, we describe use dry powder formulation (GelVac™) with an situ gelling polysaccharide (GelSite™) extracted from Aloe vera for nasal...
Abstract Emergence of SARS-CoV-2 variants and waning vaccine/infection-induced immunity pose threats to curbing the COVID-19 pandemic. Effective, safe, convenient booster vaccines are in need. We hypothesized that a variant-modified mucosal vaccine might induce local prevent infection at port entry. The beta-variant is one hardest cross-neutralize. Herein, we assessed protective efficacy an intranasal composed beta variant-spike protein S1 with IL-15 TLR agonists previously immunized...
ABSTRACT Tuberculosis (TB) is a major infectious disease problem: 1.7 million people annually die due to TB. Emergence of drug-resistant Mycobacterium tuberculosis and the lack new antibiotics have exacerbated situation. There an urgent need develop or repurpose drugs against We evaluated inhaled gentamicin as direct respiratory system-targeted therapy in murine model Aerosolized-gentamicin-treated mice showed significantly reduced lung M. loads fewer granulomas relative untreated controls....
Abstract Strategy, Management and Health Policy Enabling Technology, Genomics, Proteomics Preclinical Research Development Toxicology, Formulation Drug Delivery, Pharmacokinetics Clinical Phases I‐III Regulatory, Quality, Manufacturing Postmarketing Phase IV Accidental or deliberate release of plutonium ( P u) americium A m) into the environment could lead to internal contamination by dermal exposure, ingestion, inhalation result in severe health risks exposed human population. Therefore, it...
Abstract The aim of this study was to evaluate if the permeability inhaled corticosteroids entering brain is reduced and P-glycoprotein (P-gp) transporters are involved. Currently employed were given intravenously intratracheally rats at a dose 100 μg kg−1. An ex-vivo receptor binding assay used monitor over 12 h glucocorticoid occupancy in systemic reference organ (kidney). involvement P-gp triamcinolone acetonide assessed wild-type mice mdr1a(-/-) knockout (mice lacking gene for expressing...
Novel decorporation agents are being developed to protect against radiological accidents and terrorists attacks. Radioactive americium is a significant component of nuclear fallout. Removal large radioactive materials, such as 241Am, from exposed persons subject interest due the hazards they pose. The objective this study was evaluate dose-related efficacy daily doses NanoDTPA™ Capsules for decorporating Am administered intravenously soluble citrate complex male female beagle dogs. In...
Atopic Dermatitis (AD) is characterized by skin barrier disruption and an aberrant immune response. Doxycycline tetracycline antibiotics broadly used systemically to treat inflammatory dermatologic conditions. Several studies have shown doxycycline has anti-inflammatory pro-healing properties, mainly blocking tissue proteolytic activity. It our hypothesis that daily application of a novel topical formulation in AD subjects will reduce severity the disease, cutaneous proteases activity...
Polymer/layered silicate nanocomposite thin films were developed as clear, transparent coatings. Laponite‐S, which is a synthetic layered clay, was dispersed in polyethylene oxide matrix and solution cast onto glass slides for further testing. Rheological properties, wear abrasion resistance, scratch resistance tested each sample to examine the nanoparticle dispersion effects on materials' properties. In this study, we identify characterize materials be used, establish appropriate conditions...
We established a novel brain slice assay to test the ability of acetylcholinesterase (AChE) reactivators prevent ACh-induced M1 muscarinic acetylcholine receptor (mAChR) dependent hyperexcitability observed after exposure organophosphate (OP)-based AChE inhibitor and sarin surrogate 4-nitrophenyl isopropyl methylphosphonate (NIMP). Whole-cell patch clamp recordings were used evaluate response pyramidal neurons in rat basolateral amygdala (BLA) brief (1 min) bath application ACh (100 μM),...
Abstract The development of medical countermeasures (MCMs) against organophosphate (OP) induced poisoning is substantial importance. Use conventional therapeutics complicated by off-target effects and restricted penetration the blood-brain barrier (BBB). Therefore, a concerted effort underway to discover improved acetylcholinesterase (AChE) reactivators, muscarinic acetylcholine receptor (mAChR) antagonists, other with broader spectrum activity enhanced CNS efficacy. We recently developed...
Abstract PRX-3140 is a partial agonist to the 5-hydroxytryptamine receptor 4 (5-HT4) and ligand for sigma-1 (S1R) sigma-2 (S2R) receptors. Although few publications have inferred S1R agonists/antagonists modulate blood glucose, Di et.al (2017) reported deficiency in knockout mice impacted regulation of hypothalamic-pituitary-adrenocortical (HPA) axis, with dexamethasone-induced reduction level corticosterone markedly attenuated −/− mice, implicating feedback response HPA axis. The hypothesis...
The development of medical countermeasures (MCMs) against organophosphate (OP) induced poisoning is substantial importance. Use conventional therapeutics complicated by off-target effects and restricted penetration the blood-brain barrier (BBB). Therefore, a concerted effort underway to discover improved acetylcholinesterase (AChE) reactivators, muscarinic acetylcholine receptor (mAChR) antagonists, other with broader spectrum activity enhanced CNS efficacy. We recently developed rat brain...
Abstract Systemic vaccination of macaques with V1-deleted (ΔV1) envelope immunogens reduce the risk SIV mac251 acquisition by approximately 60%, protective roles played V2-specific ADCC and envelope-specific mucosal IL-17 + NKp44 innate lymphoid cells (ILCs). We investigated whether increased responses to V2 benefit vaccine efficacy delivering oral nanoparticles (NPs) that release V2-scaffolded on Typhoid Toxin B (TTB) large intestine. Strikingly, immunization male abrogated control TTB or...