A5015087586- Animal Disease Management and Epidemiology
- Tissue Engineering and Regenerative Medicine
- Vector-Borne Animal Diseases
- Electrospun Nanofibers in Biomedical Applications
- Viral gastroenteritis research and epidemiology
- Mesenchymal stem cell research
- Poxvirus research and outbreaks
- Animal Virus Infections Studies
- Virus-based gene therapy research
- T-cell and Retrovirus Studies
- Hepatitis Viruses Studies and Epidemiology
- Viral Infections and Vectors
- Infectious Diseases and Mycology
- Mosquito-borne diseases and control
- Silk-based biomaterials and applications
- Pluripotent Stem Cells Research
- Cervical Cancer and HPV Research
- Herpesvirus Infections and Treatments
- Aquaculture disease management and microbiota
- Marine Ecology and Invasive Species
- Renal and related cancers
- Sesquiterpenes and Asteraceae Studies
- Veterinary Equine Medical Research
- Phytoplasmas and Hemiptera pathogens
- Viral Infections and Immunology Research
Istituto Zooprofilattico Sperimentale Della Sicilia
2013-2022
Institute for Veterinary Medical Research
2015
University of Bologna
2015
Acobiom (France)
2015
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2015
Autologous AD-MSC [adipose-derived MSC (mesenchymal stem cell)] therapy involves harvesting fat from the patient by isolating and regenerative cells administering back to patient. This study evaluated production of canine AD-MSCs their possible application in cellular for dogs. To assess whether can replace drug therapy, clinical effect a single intra-articular injection was on 4 dogs with lameness associated OA (osteoarthritis) humeroradial joints. MSCs were readily isolated adult dog...
Triple-negative breast cancers (TNBCs) are clinically aggressive forms associated with a poor prognosis. We evaluated the cytotoxic effect exerted on triple-negative MDA-MB231 cancer cells both by parthenolide and its soluble analogue dimethylamino (DMAPT) explored underlying molecular mechanism. The drugs induced dose- time-dependent decrement in cell viability, which was not prevented caspase inhibitor z-VAD-fmk. In particular first hours of treatment (1–3 h), DMAPT strongly stimulated...
Enteric viruses are responsible for foodborne and waterborne infections affecting a large number of people. Data on food water viral contamination in the south Italy (Sicily) scarce fragmentary. The aim this study was to evaluate presence food, samples, surface swabs collected Sicily METHODS: survey conducted 108 shellfish, 23 samples (seawater, pipe water, torrent water), 52 vegetables, one peach 17 berries, 11 gastronomic preparations containing fish products and/or raw 28 swabs. Hepatitis...
Abstract Adipose tissue as a stem cell source is ubiquitously available and has several advantages compared to other sources, for example it easily accessible in large quantities with minimal invasive harvesting procedure, isolation of adipose‐derived mesenchymal cells (MSCs) yields high amount cells, essential cell‐based therapies engineering. We have explored the effect donor age, anatomical origin adipose on aspects MSCs dogs, such yield, proliferative ability, multi‐differentiation...
Abstract Osteosarcoma is the second leading cause of cancer‐related death for children and young adults. In this study, we have subcutaneously injected—with without matrigel—athymic mice (Fox1nu/nu) with human osteosarcoma 3AB‐OS pluripotent cancer stem cells (CSCs), which previously isolated from MG63 cells. Engrafted were highly tumorigenic matrigel greatly accelerated both tumor engraftment growth rate. CSC xenografts lacked crucial regulators beta‐catenin levels (E‐cadherin, APC,...
The use of three-dimensional (3D) cultures may induce cardiac progenitor cells to synthesize their own extracellular matrix (ECM) and sarcomeric proteins initiate differentiation. 3D grown on synthetic scaffolds favour the implantation survival stem for cell therapy when pharmacological therapies are not efficient in curing cardiovascular diseases organ transplantation remains only treatment able rescue patient's life. Silk fibroin-based be used increase affinity biomaterials chemically...
Canine distemper virus (CDV) is the etiologic agent of in dogs. It exhibits an elevated potential crossing species barriers, infecting a wide range wild and domestic carnivores. Of its encoding genes, hemagglutinin (H) shows high heterogeneity, it was used to determine relationship between CDV strains due variability key role determining cell tropism, host shift, eliciting protective immune response. This study analysed full-length H gene sequence Arctic-like collected from dogs Italy during...
The genus Pestivirus, which belongs to the Flaviviridae family, includes ssRNA+ viruses responsible for infectious diseases in pigs, cattle, sheep, goats and other domestic wild ruminants. Like most of RNA viruses, pestivirus has high genome variability with practical consequences on disease epidemiology, diagnosis control. In addition officially recognized species such as BVDV-1, BVDV-2, BDV CSFV, pestiviruses have been detected. Furthermore, ruminant show low or absent specificity observed...
A proliferative cauliflower lesion was excised from the udder of a sheep. Histological investigation confirmed macroscopic classification as papilloma, without any fibroblastic proliferation. PCR revealed presence bovine papillomavirus (BPV), which further by identification Deltapapillomavirus4 Next Generation Sequencing analysis. This subsequently classified type 1. Negative staining electron microscopy (EM) analyses produced negative test results for particles. RNA in situ hybridization...
Our study describes a newly developed mini-array test for the rapid detection of poxviruses in animals and humans. The method is based on that combines target nucleic acid amplification by polymerase chain reaction specific hybridization, using enzyme-linked antibodies, allowing identification zoonotic orthopoxviruses parapoxviruses animal human biological samples. With 100% specificity, rules out possibility cross-reactions with viral agents causing look-alike diseases. assay was employed...
In this study, mesenchymal stem cells were isolated from rat adipose tissue (AD-MSCs) to characterize and differentiate them into endothelial-like cells. AD-MSCs by mechanical enzymatic treatments, their identity was verified colony-forming units (CFU) test differentiation of mesodermal lineages. The endothelial induced plating another aliquot in EGM-2 medium, enriched with specific growth factors. Five subcultures performed. expression stemness genes (OCT4, SOX2 NANOG) investigated....
Mesenchymal stem cells (MSCs) are used in therapy animal models and veterinary medicine, due to their capacity of inducing tissue regeneration immunomodulation. Their clinical application requires a ready off-the-shelf amount viable therapeutics doses. For this purpose, it is useful cryopreserve MSCs gain controlled source abundant autologous cells. We evaluated the effect 7 years cryopreservation using 10% dimethyl sulfoxide (DMSO) with different fetal bovine serum (FBS) concentrations...
The production of a cellularized silk fibroin scaffold is very difficult because it actually impossible to differentiate cells into well-organized cardiac tissue. Without vascularization, not only do cell masses fail grow, but they may also exhibit an area necrosis, indicating lack oxygen and nutrients. In the present study, we used so-called tyrosine protein kinase kit (c-Kit)-positive progenitor (CPCs) generate scaffolds, multipotent isolated from adult heart date that can show some degree...
Bluetongue (BT) is an orbiviral disease of wild and domestic ruminants, mainly sheep. In Sicily, the first outbreak occurred in October 2000; there have been 76 recorded outbreaks so far. The National Surveillance Plan, based on European Union Commission Decision 138/2001/CE, establishes serological entomological surveys. This plan consists controls seronegative cattle, called 'sentry' as indicators for presence circulation virus defined areas. To check seroconversions, regional territory...
Enteric viruses are responsible for foodborne and waterborne infections caused by contaminated food products water, affecting a large number of people.Among the foods an important role is played shellfish, on ground that they can be eaten raw or undercooked.Data water viral contamination in South Italy scarce fragmentary.As illnesses result from failure to control hazard, aim this study was detect main pathogenic human enteric environment, evaluating presence shellfish samples collected...
Background: The families Papillomaviridae and Poxviridae include a number of species affecting different mammal species. Among Poxviridae, the genus Parapoxvirus Orthopoxvirus, viruses causing skin lesions in ruminants as well humans. These infect via damaged give rise to pustular occasionally buccal mucosa. family (PV) now comprises 29 genera among which several viral types ruminants. During last few years, massive cutaneous had been reported cows from Sicily, our study was aimed at...
Background: Numerous viruses of human or animal origin can spread in the environment and infect people. Foodborne waterborne infections cause outbreaks effecting a large number people are common gastroenteritis. Among foods, an important role is played by raw undercooked shellfishes vegetables. The aim this study was to evaluate presence viral contamination some food water samples collected South Italy (Sicily). Methods & Materials: survey conducted over period five years (2013–2017). A...