A5081037308- Advanced Fluorescence Microscopy Techniques
- Force Microscopy Techniques and Applications
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Advanced Electron Microscopy Techniques and Applications
- Cell Image Analysis Techniques
- Nanofabrication and Lithography Techniques
- Genetics and Neurodevelopmental Disorders
- Malaria Research and Control
- Advanced Materials Characterization Techniques
- Fetal and Pediatric Neurological Disorders
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Molecular Biology Techniques and Applications
- Mosquito-borne diseases and control
- Digital Imaging for Blood Diseases
Freie Universität Berlin
2019-2024
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
2019
Expansion microscopy (ExM) is a recently developed technique that allows for the resolution of structures below diffraction limit by physically enlarging hydrogel-embedded facsimile biological sample. The target structure labeled and this label must be retained in relative position true to original, smaller state before expansion linking it into gel. However, gel formation digestion lead significant loss target-delivered label, resulting weak signal. To overcome problem, we have here an...
The combination of image analysis and superresolution microscopy methods allows for unprecedented insight into the organization macromolecular assemblies in cells. Advances deep learning (DL)-based object recognition enable automated processing large amounts data, resulting high accuracy through averaging. However, while highly symmetric structures constant size a resolution approaching dimensions structural biology, DL-based may introduce bias. This prohibits development readouts processes...
Summary Mammalian cytokinesis critically depends on the phospholipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P 2 ] which serves as docking site for crucial components of cytokinetic machinery at plasma membrane. PI(4,5)P supports several stages cytokinesis, including actomyosin ring assembly and constriction, membrane tethering spindle microtubules, midbody organization. How these various activities underlying mechanisms local synthesis are orchestrated in space time has remained...
Abstract The combination of image analysis and fluorescence superresolution microscopy methods allows for unprecedented insight into the organization macromolecular assemblies in cells. Advances deep learning-based object recognition enables automated processing large amounts data, resulting high accuracy through averaging. However, while highly symmetric structures constant size a resolution approaching dimensions structural biology, learning are prone to different forms bias. A biased may...
Abstract Expansion microscopy (ExM) is a recently developed technique that allows for the resolution of structures below diffraction limit by physically enlarging hydrogel‐embedded facsimile biological sample. The target structure labeled and this label must be retained in relative position true to original, smaller state before expansion linking it into gel. However, gel formation digestion lead significant loss target‐delivered label, resulting weak signal. To overcome problem, we have...