Lipopolysaccharide (LPS) is a component of the outer membrane Gram-negative bacteria that induces strong proinflammatory reactions mammals. These processes are triggered upon sequential binding LPS to CD14, GPI-linked plasma raft protein, and TLR4/MD2 receptor complex. We have found earlier binding, CD14 triggers generation phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], lipid controlling subsequent cytokine production. Here we show stimulation RAW264 macrophage-like cells with global...

Diacylglycerol kinase-ε (DGKε) is a unique member of the DGK family with strict specificity toward DAG containing stearic and arachidonic fatty acid residues, called SAG, producing phosphatidic used for synthesis phosphatidylinositol (PI). PI its derivatives, both phosphorylated non-phosphorylated ones, regulate multitude processes, including signaling diverse plasma membrane receptors. To latter belong Toll-like receptor 4 (TLR4) accessory CD14 protein activated in macrophages by bacterial...

Benzoxazinoids (BX) are major secondary metabolites of gramineous plants that play an important role in disease resistance and allelopathy. They also have many other unique properties including anti-bacterial anti-fungal activity, the ability to reduce alfa-amylase activity. The biosynthesis modification BX controlled by genes Bx1 ÷ Bx10, GT glu, majority these Bx been mapped maize, wheat rye. However, genetic basis remains largely uncharacterized apart from some data maize wheat. aim this...

Abstract LPS binds sequentially to CD14 and TLR4/MD2 receptor triggering production of proinflammatory mediators. The LPS-induced signaling is controlled by a plasma membrane lipid PI(4,5)P2 its derivatives. Here, we show that stimulation murine peritoneal macrophages with induces biphasic accumulation peaks at 10 60–90 min were still seen after silencing TLR4 expression. In contrast, the elevation was abrogated when removed from cell surface. To assess contribution changes, used HEK293...

Abstract TLR4 is activated by the bacterial endotoxin lipopolysaccharide (LPS) and triggers two proinflammatory signaling cascades: a MyD88‐dependent one in plasma membrane, following TRIF‐dependent endosomes. An inadequate inflammatory reaction can be detrimental for organism leading to sepsis. Therefore, novel approaches therapeutic modulation of are being sought after. The activity tightly connected with presence CD14, GPI‐anchored protein that transfers LPS monomers receptor controls its...

Abstract This study was conducted in order to identify quantitative trait loci (QTLs) for the vitro culture response of winter rye (Secale cereale L.) immature embryos and inflorescences. A genetic linkage map comprising 67 SSRs, 9 ISSRs, 13 SAMPLs, 7 RAPDs, 2 SCARs one EST marker created based on analyses 102 recombinant inbred lines from cross between L318 (which has a good tissue cultures) L9 is unable regenerate plants somatic tissues anthers). The spans 979.2 cM, average distance...

Lipopolysaccharide (LPS) induces a strong pro-inflammatory reaction of macrophages upon activation Toll-like receptor 4 (TLR4) with the assistance CD14 protein. Considering key role plasma membrane rafts in and TLR4 activity significant impact exerted on that by endocytosis intracellular trafficking both LPS acceptors, it seemed likely could be modulated flotillins. Flotillin-1 -2 are scaffolding proteins associated also endo-membranes, affecting dynamics protein trafficking. To verify above...

Lipopolysaccharide (LPS) is the component of Gram-negative bacteria that activates Toll-like receptor 4 (TLR4) to trigger proinflammatory responses. We examined involvement Lyn tyrosine kinase in TLR4 signaling macrophages, distinguishing its catalytic activity and intermolecular interactions. For this, a series Lyn-GFP constructs bearing point mutations particular domains were overexpressed RAW264 macrophage-like cells or murine peritoneal their influence on LPS-induced responses was...

Abstract Bacterial LPS strongly induces pro-inflammatory responses of Mϕs after binding to CD14 protein and the TLR4/MD-2 receptor complex. The LPS-triggered signaling can be modulated by extracellular lysophosphatidic acid (LPA), which is substantial importance for Mϕ functioning under specific pathophysiological conditions, such as atherosclerosis. molecular mechanisms crosstalk between LPS- LPA-induced signaling, LPA receptors involved, are poorly known. In this report, we show that...

Palmitic acid (C16:0) is the most abundant saturated fatty in animals serving as a substrate synthesis and β-oxidation of other lipids, modification proteins called palmitoylation. The influence dietary palmitic on protein S-palmitoylation remains largely unknown. In this study we performed high-throughput proteomic analyses membrane-enriched fraction murine liver to examine palm oil-rich diet (HPD) proteins. HPD feeding for 4 weeks led an accumulation C16:0 C18:1 acids livers which...

Diacylglycerol kinase-ε (DGKε) phosphorylates DAG to phosphatidic acid with unique specificity toward 18:0/20:4 (SAG). SAG is a typical backbone of phosphatidylinositol and its derivatives, therefore DGKε activity crucial for the turnover these signaling lipids. Malfunction contributes several pathophysiological conditions, including atypical hemolytic uremic syndrome (aHUS) linked DGKE mutations. In present study we analyzed role zinc finger motif C1B domain DGKε, as some aHUS-linked...

Diacylglycerol kinase-ε (DGKε) catalyzes phosphorylation of diacylglycerol to phosphatidic acid with a unique specificity toward 1-stearoyl-2-arachidonoyl-sn-glycerol which is backbone phosphatidylinositol (PI). Owing this specificity, DGKε involved in the PI cycle maintaining cellular level phosphorylated derivatives signaling activity, and was also found crucial for lipid metabolism. dysfunction linked development atypical hemolytic uremic syndrome possibly other human diseases. Despite...

Abstract We present a simple subtraction procedure of GDDSC as modification the original DSC and GDRDA methods. Genetically Directed Differential Subtraction Chain (GDDSC) is process by which highly related genomes are compared in order to isolate tags carrying polymorphisms.To detect specific DNA fragment (tag), we can then monitor offspring plants for efficient molecular breeding. The protocol was applied isolation new sex clones from cucumber ( Cucumis sativus L.) tissue culture response...

Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)

Abstract Diacylglycerol kinase-ε (DGKε) catalyzes phosphorylation of diacylglycerol to phosphatidic acid with a unique specificity toward 1-stearoyl-2-arachidonoyl- sn -glycerol which is backbone phosphatidylinositol (PI). Owing this specificity, DGKε involved in the PI cycle maintaining cellular level phosphorylated derivatives signaling activity, and was also found crucial for lipid metabolism. dysfunction linked development atypical hemolytic uremic syndrome possibly other human diseases....

Abstract Lipopolysaccharide induces a strong pro-inflammatory reaction of macrophages upon activation Toll-like 4 receptor (TLR4) with the assistance CD14 protein. Considering key role plasma membrane rafts in and TLR4 activity significant impact exerted on that by endocytosis intracellular trafficking both LPS acceptors, it seemed likely could be modulated flotillins. Flotillin-1 -2 are scaffolding proteins associated also endo-membranes, affecting dynamics protein trafficking. To verify...

We present a simple subtraction procedure of GDDSC as modification the original DSC and GDRDA methods. Genetically Directed Differential Subtraction Chain (GDDSC) is process by which highly related genomes are compared in order to isolate tags carrying polymorphisms.To detect specific DNA fragment (tag), we can then monitor offspring plants for efficient molecular breeding. The protocol was applied isolation new sex clones from cucumber (Cucumis sativus L.) tissue culture response rye...