A5046388377- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- SARS-CoV-2 detection and testing
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- CAR-T cell therapy research
- Respiratory viral infections research
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Poxvirus research and outbreaks
- Brucella: diagnosis, epidemiology, treatment
- Bacillus and Francisella bacterial research
- T-cell and Retrovirus Studies
- Vector-Borne Animal Diseases
- Herpesvirus Infections and Treatments
- Viral Infections and Immunology Research
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Galectins and Cancer Biology
Kansas State University
2020-2024
University of Florida
2014-2020
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of Disease 2019 (COVID-19) and responsible for current pandemic. Recent SARS-CoV-2 susceptibility studies in cats show that virus can replicate these companion animals transmit to other cats. Here, we present an in-depth study infection, disease transmission domestic Cats were challenged with via intranasal oral routes. One day post challenge (DPC), two sentinel introduced. Animals monitored clinical signs,...
SARS-CoV-2 was first reported circulating in human populations December 2019 and has since become a global pandemic. Recent history involving SARS-like coronavirus outbreaks have demonstrated the significant role of intermediate hosts viral maintenance transmission. Evidence natural infection experimental infections wide variety animal species been demonstrated,
The emergence of SARS-CoV-2 has resulted in an ongoing global pandemic with significant morbidity, mortality, and economic consequences. susceptibility different animal species to is concern due the potential for interspecies transmission, requirement pre-clinical models develop effective countermeasures. In current study, we determined ability (i) replicate porcine cell lines, (ii) establish infection domestic pigs via experimental oral/intranasal/intratracheal inoculation, (iii) transmit...
SARS-CoV-2 is the causative agent of COVID-19 and responsible for current global pandemic. We others have previously demonstrated that cats are susceptible to infection can efficiently transmit virus naïve cats. Here, we address whether exposed be re-infected with SARS-CoV-2. In two independent studies, SARS-CoV-2-infected were re-challenged at 21 days post primary challenge (DPC) necropsies performed 4, 7 14 post-secondary (DP2C). Sentinels co-mingled 1 DP2C. Clinical signs recorded, nasal,...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for a global pandemic that has had significant impacts on human health and economies worldwide. SARS-CoV-2 highly transmissible the cause of disease 2019 in humans. A wide range animal species have also been shown to be susceptible by experimental and/or natural infections. Sheep are commonly farmed domestic ruminant not thoroughly investigated their susceptibility SARS-CoV-2. Therefore, we performed vitro vivo...
Abstract Natural killer T (NKT) -cells activated with the glycolipid ligand α-galactosylceramide (α-GalCer) stimulate a wide array of immune responses many promising immunotherapeutic applications, including enhancement vaccines against infectious diseases and cancer. In current study, we evaluated whether α-GalCer generates protective immunity swine influenza (SI) virus infection when applied as an intramuscular vaccine adjuvant. Immunization newly weaned piglets UV-killed pandemic H1N1...
Abstract Swine influenza A viruses (IAV) are a major cause of respiratory disease in pigs and humans. Currently approved anti-influenza therapies directly target the virus, but these approaches losing effectiveness as new viral strains quickly develop drug resistance. To over come this challenge, there is an urgent need for more effective antiviral drugs. Here we tested efficacy invariant natural killer T (NKT) cell superagonist, α-galactosylceramide (α-GalCer), which stimulates wide array...
Abstract Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of Disease 2019 (COVID-19) and responsible for current pandemic. Recent SARS-CoV-2 susceptibility transmission studies in cats show that virus can replicate these companion animals transmit to other cats. Here, we present an in-depth study infection, associated disease dynamics domestic Six 4- 5-month-old were challenged with via intranasal oral routes simultaneously. One day post challenge (DPC), two sentinel...
Abstract SARS-CoV-2, a novel Betacoronavirus , was first reported circulating in human populations December 2019 and has since become global pandemic. Recent history involving SARS-like coronavirus outbreaks (SARS-CoV MERS-CoV) have demonstrated the significant role of intermediate reservoir hosts viral maintenance transmission cycles. Evidence SARS-CoV-2 natural infection experimental infections wide variety animal species been demonstrated, silico vitro studies indicated that deer are...
Swine represent the only livestock with an established invariant NKT (iNKT) cell-CD1d system. In this study, we exploited fact that pig iNKT cells can be purified using a mouse CD1d tetramer reagent to establish their TCR repertoire by next generation sequencing. tetramer-positive predominantly expressed Vα-Jα rearrangement, without nontemplate nucleotide diversity, homologous Vα24-Jα18 and Vα14-Jα18 rearrangements of human murine cells. The coexpressed β-chain used Vβ segment semivariant...
The emergence of SARS-CoV-2 has resulted in an ongoing global pandemic with significant morbidity, mortality, and economic consequences. susceptibility different animal species to is concern due the potential for interspecies transmission, requirement pre-clinical models develop effective countermeasures. In current study, we determined ability (i) replicate porcine cell lines, (ii) establish infection domestic pigs via experimental oral/intranasal/intratracheal inoculation, (iii) transmit...
Influenza virus infections are a major cause of respiratory disease in humans. Neuraminidase inhibitors (NAIs) the primary antiviral medication used to treat ongoing influenza infections. However, NAIs not always effective for controlling shedding and lung inflammation. Other concerns emergence NAI-resistant strains risk side effects, which occasionally severe. Consequently, additional anti-influenza therapies replace or combine with desirable. Here, we compared efficacy NAI oseltamivir...
African swine fever (ASF) is an infectious viral disease caused by virus (ASFV), that causes high mortality in domestic and wild boar (Sus scrofa). Currently, outbreaks are mitigated through strict quarantine measures the culling of affected herds, resulting massive economic losses to global pork industry. In 2019, ASFV outbreak was reported Mongolia, describing a rapidly progressing clinical gross lesions consistent with acute form ASF; identified as genotype II virus. Due limited...
Proteolytic activation of the hemagglutinin (HA) glycoprotein by host cellular proteases is pivotal for influenza A virus (IAV) infectivity. Highly pathogenic avian viruses possess multibasic cleavage site HA which cleaved ubiquitous proteases, such as furin; in contrast, monobasic motif recognized and activated trypsin-like transmembrane serine protease 2 (TMPRSS2). Here, we aimed to determine effects TMPRSS2 on replication pandemic H1N1 H3N2 subtype IAVs natural host, pig. The use...
Abstract Natural killer T (NKT) cells activated with the glycolipid ligand α-galactosylceramide (α-GalCer) stimulate a wide variety of immune that enhance vaccine-mediated responses. Several studies have used this approach to adjuvant inactivated and subunit influenza A virus (IAV) vaccines, including cross-protective immunity. However, less is known about whether α-GalCer can live attenuated (LAIV) which usually induce superior heterologous heterosubtypic immunity compared non-replicating...
Proteolytic activation of the hemagglutinin (HA) glycoprotein by host cellular proteases is pivotal for influenza A virus (IAV) infectivity. Highly pathogenic avian viruses possess multibasic cleavage site HA which cleaved ubiquitous proteases, such as furin; in contrast, monobasic motif recognized and activated trypsin-like transmembrane serine protease 2 (TMPRSS2). Here, we aimed to determine effects TMPRSS2 on replication pandemic H1N1 H3N2 subtype IAVs natural host, pig. The use...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for a global pandemic that has had significant impacts on human health and economies worldwide. SARS-CoV-2 highly transmissible the cause of disease 2019 (COVID-19) in humans. A wide range animal species have also been shown to be susceptible infection by experimental and/or natural infections. Domestic large cats, mink, ferrets, hamsters, deer mice, white-tailed deer, non-human primates susceptible, whereas...
Abstract SARS-CoV-2 is the causative agent of COVID-19 and responsible for current global pandemic. We others have previously demonstrated that cats are susceptible to infection can efficiently transmit virus naïve cats. Here, we address whether exposed be re-infected with SARS-CoV-2. In two independent studies, SARS-CoV-2-infected were re-challenged at 21 days post primary challenge (DPC) necropsies performed 4, 7 14 post-secondary (DP2C). Sentinels co-mingled 1 DP2C. Clinical signs...
Invariant natural killer T (iNKT) cells are glycolipid-reactive with potent immunoregulatory properties. iNKT activated the marine-sponge-derived glycolipid, α-galactosylceramide (αGC), provide a universal source of T-cell help that has shown considerable promise for wide array therapeutic applications. This includes harnessing iNKT-cell-mediated immune responses to adjuvant whole inactivated influenza virus (WIV) vaccines. An important concern WIV vaccines is under certain circumstances,...
The objective of this work was to evaluate the safety and efficacy a recombinant, subunit SARS-CoV-2 animal vaccine in cats against virulent challenge. Two groups were immunized with two doses either recombinant spike protein or placebo, administered three weeks apart. Seven after second vaccination, both challenged via intranasal oral routes simultaneously. Animals monitored for 14 days post-infection clinical signs viral shedding before being humanely euthanized evaluated macroscopic...