A5044400585- Advanced Electron Microscopy Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- RNA modifications and cancer
- Amino Acid Enzymes and Metabolism
- RNA and protein synthesis mechanisms
- Ga2O3 and related materials
- Metabolism, Diabetes, and Cancer
- Receptor Mechanisms and Signaling
- Nitric Oxide and Endothelin Effects
- Iron oxide chemistry and applications
- Parathyroid Disorders and Treatments
Massachusetts Institute of Technology
2023
Pomona College
2020
Vanderbilt University
2020
Throughout the history of electron microscopy, ribosomes have served as an ideal subject for imaging and technological development, which in turn has driven our understanding ribosomal biology. Here, we provide a historical perspective at intersection microscopy technology development ribosome biology reflect on how this technique shed light each stage life cycle dynamic macromolecular machine. With emphasis prokaryotic systems, specifically describe pairing cryo-EM with thoughtful...
Abstract Arrestins demonstrate strong preference for phosphorylated over unphosphorylated receptors, but how arrestins “sense” receptor phosphorylation is unclear. A conserved lysine in the lariat loop of directly binds phosphate crystal structures activated arrestin‐1, ‐2, and ‐3. The supplies two negative charges to central polar core, which must be disrupted arrestin activation high‐affinity binding. Therefore, we hypothesized that receptor‐attached phosphates pull via this lysine, thus...
In cryogenic electron microscopy (cryoEM), purified macromolecules are applied to a grid bearing holey carbon foil; the molecules then blotted remove excess liquid and rapidly frozen in roughly 20-100 nm thick layer of vitreous ice, suspended across 1 µm wide foil holes. The resulting sample is imaged using transmission microscopy, after image processing suitable software, near-atomic resolution structures can be determined. Despite cryoEM's widespread adoption, preparation remains severe...
In cryogenic electron microscopy (cryo-EM), purified macromolecules are typically applied to a grid bearing holey carbon foil, blotted remove excess liquid and rapidly frozen in roughly 20-100 nm thick layer of vitreous ice that is suspended across 1 μm-wide foil holes. The resulting sample then imaged using transmission and, after substantial image processing, near-atomic resolution structures can be determined. Despite cryo-EM's widespread adoption, preparation remains severe bottleneck...
Throughout the history of electron microscopy, ribosomes have served as an ideal subject for imaging and technological development, which in turn has driven our understanding ribosomal biology. Here, we provide a historical perspective at intersection microscopy technology development ribosome biology reflect on how this technique shed light each stage life cycle dynamic macromolecular machine. With emphasis prokaryotic systems, specifically describe pairing cryo-EM with clever experimental...