A5044357759- Mitochondrial Function and Pathology
- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Memory and Neural Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Neuroscience and Neural Engineering
- Epilepsy research and treatment
- Lipid Membrane Structure and Behavior
- Nicotinic Acetylcholine Receptors Study
- Neuroendocrine regulation and behavior
- Advanced Memory and Neural Computing
Biomedical Research Institute
2020-2025
Virginia Tech
2020-2025
Carilion Roanoke Memorial Hospital
2021-2023
University of California System
2019
University of California, Davis
2014
Abstract Neuronal mitochondria are diverse across cell types and subcellular compartments in order to meet unique energy demands. While essential for synaptic transmission plasticity, the mechanisms regulating support normal synapse function incompletely understood. The mitochondrial calcium uniporter (MCU) is proposed couple neuronal activity ATP production, which would allow neurons rapidly adapt changing MCU uniquely enriched hippocampal CA2 distal dendrites compared proximal dendrites,...
Protein expansion microscopy (proExM) is a powerful technique that crosslinks proteins to swellable hydrogel physically expand and optically clear biological samples. The resulting increased resolution (~70 nm) physical separation of labeled make it an attractive tool for studying the localization subcellular organelles in densely packed tissues, such as brain. However, digestion process greatly reduce fluorescence signals making necessary optimize ExM conditions per sample specific end...
Abstract CA2 is an understudied subregion of the hippocampus that critical for social memory. Previous studies identified multiple components mitochondrial calcium uniporter (MCU) complex as selectively enriched in CA2. The MCU regulates entry into mitochondria, which turn transport and localization to active synapses. We found strikingly distal apical dendrites, precisely where neurons receive entorhinal cortical input carrying information. Furthermore, MCU‐enriched mitochondria dendrites...
Abstract We discovered a new type of dendritic spine. It is found on space-specific neurons in the barn owl inferior colliculus, site experience-dependent plasticity. Connectomic analysis revealed protrusions unusual morphology including topological holes, hence termed “toric” spines ( n = 76). More significantly, presynaptic terminals converging onto individual toric displayed numerous active zones (up to 49) derived from multiple axons 11) with incoming trajectories distributed widely...
ABSTRACT Mitochondria are dynamic organelles that morphologically and functionally diverse across cell types subcellular compartments in order to meet unique energy demands. Mitochondrial dysfunction has been implicated a wide variety of neurological disorders, including psychiatric disorders like schizophrenia bipolar disorder. Despite it being well known mitochondria essential for synaptic transmission plasticity, the mechanisms regulating support normal synapse function incompletely...
ABSTRACT Protein expansion microscopy (proExM) is a powerful technique that crosslinks proteins to swellable hydrogel physically expand and optically clear biological samples. The resulting increased resolution (~70 nm) physical separation of labeled make it an attractive tool for studying the localization subcellular organelles in densely packed tissues, such as brain. However, digestion process greatly reduces fluorescence signals making necessary optimize ExM conditions per sample...
ABSTRACT CA2 is an understudied subregion of the hippocampus that critical for social memory. Previous studies identified multiple components mitochondrial calcium uniporter (MCU) complex as selectively enriched in CA2, however functional significance this enrichment remains unclear. The MCU regulates entry into mitochondria, which turn transport and localization to active synapses. We found strikingly distal apical dendrites, precisely where neurons receive entorhinal cortical input...