A5039324676- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Immune cells in cancer
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Histone Deacetylase Inhibitors Research
- Receptor Mechanisms and Signaling
- Nanowire Synthesis and Applications
- Muscle metabolism and nutrition
- Publishing and Scholarly Communication
- Advancements in Semiconductor Devices and Circuit Design
- Adenosine and Purinergic Signaling
- Cancer Immunotherapy and Biomarkers
University of California, San Francisco
2022-2023
Gladstone Institutes
2023
University of California, Los Angeles
2019-2021
Targeting tumor-associated macrophages (TAMs) is a promising strategy to modify the immunosuppressive tumor microenvironment and improve cancer immunotherapy. Monoamine oxidase A (MAO-A) an enzyme best known for its function in brain; small molecule MAO inhibitors (MAOIs) are clinically used treating neurological disorders. Here we observe MAO-A induction mouse human TAMs. MAO-A-deficient mice exhibit decreased TAM functions corresponding with enhanced antitumor immunity. MAOI treatment...
Precise targeting of large transgenes to T cells using homology-directed repair has been transformative for adoptive cell therapies and biology. Delivery DNA templates via adeno-associated virus (AAV) greatly improved knockin efficiencies, but the tropism current AAV serotypes restricts their use human employed in immunodeficient mouse models. To enable targeted knockins murine cells, we evolved Ark313, a synthetic that exhibits high transduction efficiency cells. We performed genome-wide...
T cells demand massive energy to combat cancer; however, the metabolic regulators controlling antitumor cell immunity have just begun be unveiled. When studying nutrient usage of tumor-infiltrating immune in mice, we detected a sharp increase expression CrT (Slc6a8) gene, which encodes surface transporter uptake creatine into cell. Using knockout showed that deficiency severely impaired immunity. Supplementing WT mice significantly suppressed tumor growth multiple mouse models, and...
Chronic stimulation can cause T cell dysfunction and limit the efficacy of cellular immunotherapies. Improved methods are required to compare large numbers synthetic knockin (KI) sequences reprogram functions. Here, we developed modular pooled KI screening (ModPoKI), an adaptable platform for construction DNA libraries using barcoded multicistronic adaptors. We built two ModPoKI 100 transcription factors (TFs) 129 natural surface receptors (SRs). Over 30 screens across human TCR- CAR-T cells...
Monoamine oxidase A (MAO-A) restrains antitumor T cell immunity, suggesting potential use of MAO-A inhibitors in cancer immunotherapy.
SUMMARY Chronic stimulation can cause T cell dysfunction and limit efficacy of cellular immunotherapies. CRISPR screens have nominated gene targets for engineered cells, but improved methods are required to compare large numbers synthetic knockin sequences reprogram functions. Here, we developed Modular Pooled Knockin Screening (ModPoKI), an adaptable platform modular construction DNA libraries using barcoded multicistronic adaptors. We built two ModPoKI 100 transcription factors (TFs) 129...
3 Introduction 4 Problem Definition 5 The Broader Group Reflection on Vision Statement 6 Methodology 9 Findings 10 Central and Group’s Position What is Flour? 11 Types of Wheat Flour: 12 Organic Production Principles Benefits Conventional Flour Processing 13 15 Shelf Life Packaging Waste Disposal 16 Where Grains Go? Ecological Footprints 17 Health Nutrition 18 Recommendations 22 For UBC Food Services Future AGSC 450 Students 23 Conclusion 24 Appendix I 26 II 27 References 28 Consent Form 30
Abstract Targeting tumour-associated macrophages (TAMs) is a promising strategy to modify the immunosuppressive tumour microenvironment and improve cancer immunotherapy. Monoamine oxidase A (MAO-A) an enzyme best known for its function in brain; small molecule MAO inhibitors (MAOIs) are clinically used treating neurological disorders. Here we observed MAO-A induction mouse human TAMs. MAO-A-deficient mice exhibited decreased TAM functions corresponding with enhanced antitumour immunity. MAOI...