A5091879853- Enzyme Structure and Function
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Infrastructure Maintenance and Monitoring
- Geomagnetism and Paleomagnetism Studies
- Protein Kinase Regulation and GTPase Signaling
- Mass Spectrometry Techniques and Applications
- Geophysical and Geoelectrical Methods
- Ultrasonics and Acoustic Wave Propagation
- Magnetic and Electromagnetic Effects
- Radiopharmaceutical Chemistry and Applications
- HER2/EGFR in Cancer Research
- Structural Health Monitoring Techniques
University of California, Berkeley
2023-2024
University of Washington
2023-2024
Beijing University of Technology
2000-2005
Beijing Polytechnic
2000-2005
Abstract Protein–RNA and protein–DNA complexes play critical roles in biology. Despite considerable recent advances protein structure prediction, the prediction of structures protein–nucleic acid without homology to known is a largely unsolved problem. Here we extend RoseTTAFold machine learning protein-structure-prediction approach additionally predict nucleic complexes. We develop single trained network, RoseTTAFoldNA, that rapidly produces three-dimensional models with confidence...
Abstract In natural proteins, structured loops have central roles in molecular recognition, signal transduction and enzyme catalysis. However, because of the intrinsic flexibility irregularity loop regions, organizing multiple at protein functional sites has been very difficult to achieve by de novo design. Here we describe a solution this problem that designs tandem repeat proteins with (9–14 residues) buttressed extensive hydrogen bonding interactions. Experimental characterization shows...
A general approach to design proteins that bind tightly and specifically intrinsically disordered regions (IDRs) of flexible peptides would have wide application in biological research, therapeutics, diagnosis. However, the lack defined structures high variability sequence conformational preferences has complicated such efforts. We sought develop a method combining biophysical principles with deep learning readily generate binders for any sequence. Instead assuming fixed regular structure...
Abstract The proto-oncogene Ras which governs diverse intracellular pathways has four major isoforms (KRAS4A, KRAS4B, HRAS, and NRAS) with substantial sequence homology similar in vitro biochemistry. There is considerable interest investigating the roles of these independently as their association different cancers vary, but there are few isoform-specific binding reagents only significant differences disordered highly charged C-termini have been difficult to elicit antibodies against. To...
In natural proteins, structured loops play central roles in molecular recognition, signal transduction and enzyme catalysis. However, because of the intrinsic flexibility irregularity loop regions, organizing multiple at protein functional sites has been very difficult to achieve by
A new species of single-cell magnetotactic bacteria, the NMV-1 has been found by us. Each bacterium synthesizes itself a chain magnetic nanoparticles inside its body. When an external field is applied, long bacteria chains come into being in direction water. More than 30 µm live have observed. Length dependant: higher is, longer are. The orientation controllable and can be stably trapped. mechanism assembly also discussed. results show that, while strong enough interactions between each...