A5031605337- Estrogen and related hormone effects
- Cancer Cells and Metastasis
- Endometriosis Research and Treatment
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- Reproductive System and Pregnancy
- Uterine Myomas and Treatments
- Endometrial and Cervical Cancer Treatments
- Phagocytosis and Immune Regulation
- Immune cells in cancer
- Cell Adhesion Molecules Research
- Genomics and Chromatin Dynamics
- Kruppel-like factors research
- Cellular Mechanics and Interactions
- Cytokine Signaling Pathways and Interactions
- Cancer-related molecular mechanisms research
- Retinoids in leukemia and cellular processes
- HER2/EGFR in Cancer Research
- Cancer-related gene regulation
- Animal Genetics and Reproduction
- TGF-β signaling in diseases
- Ubiquitin and proteasome pathways
The University of Texas Health Science Center at San Antonio
2015-2024
The University of Texas Health Science Center at Houston
2008-2024
The University of Texas at San Antonio
2013-2022
Audie L. Murphy Memorial VA Hospital
2020
Mays Cancer Center at UT Health San Antonio
2019
Medical College of Wisconsin
2013
National Defense Medical Center
2013
The Ohio State University
2013
Tri-Service General Hospital
2013
Beijing Obstetrics and Gynecology Hospital
2007
Abstract Increasing evidence suggests the presence of minor cell subpopulations in prostate cancer that are androgen independent and poised for selection as dominant clones after deprivation therapy. In this study, we investigated phenomenon by stratifying based on transcriptome profiling 144 single LNCaP cells treated or untreated with cell-cycle synchronization. Model-based clustering 397 differentially expressed genes identified eight potential cells, revealing a previously unappreciable...
Cytometry by time-of-flight (CyTOF) simultaneously measures multiple cellular proteins at the single-cell level and is used to assess intertumor intratumor heterogeneity. This approach may be investigate variability of individual tumor responses treatments. Herein, we stratified lung subpopulations based on AXL signaling as a potential targeting strategy. Integrative transcriptome analyses were how TP-0903, an kinase inhibitor, influences redundant oncogenic pathways in metastatic cancer...
Despite recent advances in diagnosis and treatment of testicular cancer, its causes remain unknown. The most common conditions known to be associated with cancer are cryptorchidism, infertility, overexposure pesticides or radiation. Recent studies also indicate hormones may play a crucial role tumorigenesis. Our show that about half the male transgenic mice overexpressing aromatase testis were infertile and/or had larger than normal testicles. Gross pathology histological analysis showed...
Women with advanced stage ovarian cancer (OC) have a five-year survival rate of less than 25%. OC progression is associated accumulation epigenetic alterations and aberrant DNA methylation in gene promoters acts as an inactivating "hit" during initiation progression. Abnormal has been used to predict disease outcome therapy response. To globally examine OC, we next-generation sequencing technology, MethylCap-sequencing, screen 75 malignant 26 normal or benign tissues. Differential regions...
Abstract Overexpression of epithelial cell adhesion molecule (EpCAM) has been implicated in advanced endometrial cancer, but its roles this progression remain to be elucidated. In addition structural role modulating cell-surface adhesion, here we demonstrate that EpCAM is a regulatory which internalization into the nucleus turns on transcription program. Activation EGF/EGFR signal transduction triggered cleavage EpCAM, leading nuclear cytoplasmic domain EpICD. ChIP-seq analysis identified...
Aggressive tumors of epithelial origin shed cells that intravasate and become circulating tumor (CTC). The CTCs are able to survive the stresses encountered in bloodstream can then seed metastases. We demonstrated previously isolated from blood prostate cancer patients display specific nanomechanical phenotypes characteristic cell endurance invasiveness patient sensitivity androgen deprivation therapy. Here we report patient-isolated nanomechanically distinct randomly tumor, with high...
Epidemiologic studies have implicated estrogenic exposure as well human papilloma virus (HPV) infection in cervical carcinogenesis, and some suggested that estrogen HPV may play synergistic roles tumorigenesis. In this study, we report a novel finding approximately 35% of carcinomas tested (n = 19) express aromatase, the enzyme responsible for converting androgen to estrogen, rate-limiting final step biosynthesis. On other hand, no aromatase expression was detected precancerous 42) or normal...
Recent genome-wide analysis has shown that DNA methylation spans long stretches of chromosome regions consisting clusters contiguous CpG islands or gene families. Hypermethylation various been reported in many types cancer. In this study, we conducted methyl-binding domain capture (MBDCap) sequencing (MBD-seq) on a breast cancer cohort 77 patients and 10 normal controls, as well panel 38 cell lines.Bioinformatics determined seven with significant difference overall survival (OS) further...
Abstract Cervical cancer is the third most common gynecologic in United States. The presence and possible involvement of several cytokines have been studied cervical cancer; however, very little data, if any, are available on whether tumors responsive to stimulation by macrophage colony-stimulating factor-1 (CSF-1). Given c-fms its ligand CSF-1 cancers, such as that uterus ovaries, we examined expression tumor (n = 17) normal cervix 8) samples. data show significantly higher carcinomas...
Epigenetic regulation by promoter methylation plays a key role in tumorigenesis. Our goal was to investigate whether altered DNA signatures associated with oncogenic signaling delineate biomarkers predictive of endometrial cancer recurrence.Methyl-CpG-capture sequencing used for global screening aberrant our cohort, followed validation an independent The Cancer Genome Atlas (TCGA) cohort. Bioinformatics as well functional analyses vitro, using RNA interference (RNAi) knockdown, were...
The epigenome delineates lineage-specific transcriptional programs and restricts cell plasticity to prevent non-physiological fate transitions. Although diversification fosters tumor evolution therapy resistance, upstream mechanisms that regulate the stability of cancer remain elusive. Here we show 2-hydroxyglutarate (2HG) not only suppresses DNA repair but also mediates high-plasticity chromatin landscape. A combination single-cell epigenomics multi-omics approaches demonstrates 2HG...
To test directly the role of breast-tissue estrogen in initiation breast cancer, we have developed aromatase-transgenic mouse model and demonstrated for first time that increased mammary estrogens resulting from overexpression aromatase glands lead to induction various preneoplastic neoplastic changes are similar early cancer. Continued leads contributes a number epigenetic tissue such as alteration regulation genes involved apoptosis, activation cell cycle proliferation, growth factors. Our...
The aim of this study was to investigate whether activin A has an effect on the attachment and/or invasion endometrial cells in a modeled peritoneum vitro. Cultured stromal (ESCs) and epithelial (EECs) were treated with (6.25-50 ng/ml) (25 without inhibin or follistatin. Fluorescent labeled added confluent peritoneal mesothelial (PMCs) monolayer PMCs grown Matrigel assay. rate cell through assessed. expression adhesion proteins N- E-cadherin evaluated real-time RT-PCR. Activin promoted...
Transforming growth factor beta 1 (TGF-beta1) levels are increased in the peritoneal fluid of endometriosis patients, and endometrial cells express TGF-beta signaling components; however, little is known regarding role endometriosis. Our objective was to examine effects TGF-beta1 on (i) expression macrophage colony-stimulating receptor encoded by c-fms gene, (ii) transmesothelial invasiveness cells, (iii) cellular proliferation (iv) attachment mesothelial (PMCs). Effects mRNA were determined...
A number of recent studies have suggested that the colony-stimulating factor (CSF-1) and its receptor c-fms may be involved in development mammary glands during lactation breast cancer. To study role CSF-1 or initiation tumorigenesis, we generated two independent lines transgenic mice overexpress either under control mouse tumor virus promoter. Mammary virgin show increased ductal branching, hyperplasia, dysplasia, other preneoplastic changes, which are indicative cellular proliferation....
Abstract In situ estrogen synthesis is implicated in tumor cell proliferation through autocrine or paracrine mechanisms especially postmenopausal women. Several recent studies demonstrated activity of aromatase, an enzyme that plays a critical role breast tumors. Proline-, glutamic acid-, and leucine-rich protein-1 (PELP1/MNAR) receptor (ER) coregulator, its expression deregulated this study, we examined whether PELP1 promotes growth by promoting local using cancer cells (MCF7) stably...
In postmenopausal women, local estrogen produced by adipose stromal cells in the breast is believed to support receptor alpha (ERα) positive cancer cell survival and growth. This raises question of how ERα metastatic survive after they enter blood lymph circulation, where level very low women. this study, we show that aromatase expression increased when were cultured suspension. Furthermore, treatment with substrate, testosterone, inhibited suspension culture-induced apoptosis whereas an...
DNA hypermethylation of promoterCpG islands is associated with epigenetic silencing tumor suppressor genes in oral squamous cell carcinomas (OSCCs).We used a methyl-CpG-binding domain protein capture method coupled next-generation sequencing (MBDCap-seq) to survey global methylation patterns OSCCs and without nodal metastasis normal mucosa (total n = 58).Of 1462 differentially methylated CpG identified relative controls, MBDCap-seq profiling uncovered 359 loci linked lymph node...